Effects of diets with distinct protein-to-carbohydrate ratios on nutrient digestibility, growth performance, body composition and liver intermediary enzyme activities in gilthead sea bream (Sparus aurata, L.) fingerlings

Fernández, Felipe; Miquel, A.; Córdoba, Marlon; Varas, Manuel; Metón, Isidoro; Caseras, Anna; Baanante, Isabel V.

doi:10.1016/j.jembe.2006.10.057

Cited by 132 publications

(107 citation statements)

References 41 publications

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“…In Labeo rohita, the ratio between dietary non-gelatinized and gelatinized starch do not affect the glycolytic activity (Vikas et al, 2010). However, higher activities of glycolytic enzymes are observed in gilthead sea bream fed diets with gelatinized corn starch (Fernandez et al, 2007). Controversial results were posteriorly observed on the hepatic HK and PK activities (Enes et al, 2008.…”

Section: Discussionmentioning

confidence: 99%

Glycolysis and gluconeogenesis in the liver of catfish fed with different concentrations of proteins, lipids and carbohydrates

Melo

Lundstedt

Inoue

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Glycolysis and gluconeogenesis in the liver ABSTRACTThe activities of enzymes from a number of metabolic pathways have been used as a tool to evaluate the best use of nutrients on fish performance. In the present study the catfish Rhamdia quelen was fed with diets containing crude protein-lipid-carbohydrate (%) as follows: treatment (T) T1: 19-19-44; T2: 26-15-39; T3: 33-12-33; and T4: 40-10-24. The fish were held in tanks of re-circulated, filtered water with controlled temperature and aeration in 2000L experimental units. The feeding experiment lasted 30 days. The following enzymes of the carbohydrate metabolism were determined: Glucokinase (GK), Phosphofructokinase 1 (PFK-1), Pyruvate kinase (PK), Fructose-1,6-biphosphatase 1 (FBP-1). The activities of 6 phosphogluconate dehydrogenase (6PGDH) and glucose 6 phosphate dehydrogenase (G6PDH) were also assayed. The influence of nutrient levels on the enzyme activities is reported. The increase of dietary protein plus reduction of carbohydrates and lipids attenuates the glycolytic activity and induces hepatic gluconeogenesis as a strategy to provide metabolic energy from amino acids. The fish performance was affected by the concentrations of protein, lipid and carbohydrates in the diet. The greatest weight gain was obtained in fish fed diet T4 containing 40.14% of crude protein, 9.70% of lipids, and 24.37% of carbohydrate, respectively. Keywords: metabolism, enzymes, nutrition, adaptation : 19-19-44; T2: 26-15-39; T3: 33-12-33; e T4: 40-10-24 RESUMO As atividades de enzimas das vias metabólicas têm sido utilizadas como uma ferramenta para avaliar a melhor utilização dos nutrientes e o desempenho dos peixes. No presente estudo, o jundiá foi alimentado com rações contendo diferentes concentrações de proteína bruta, lipídeos e carboidratos (%), da seguinte forma: tratamento (T) T1

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Section: Discussionmentioning

confidence: 99%

Glycolysis and gluconeogenesis in the liver of catfish fed with different concentrations of proteins, lipids and carbohydrates

Melo

Lundstedt

Inoue

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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“…Therefore, the plasticity of this system determines how well a particular organism adapts to dietary CHO. For farmed carnivorous fish species such as the European seabass Dicentrarchus labrax L., there is current interest from both economic and ecological perspectives about their capacity to adapt from their natural high protein/low CHO diet to feedstock where a significant portion of protein is replaced by CHO (Fernández et al, 2007;Enes et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of food-deprivation and refeeding on the regulation and sources of blood glucose appearance in European seabass (Dicentrarchus labrax L.)

Viegas

Rito

González

et al. 2013

Comparative Biochemistry and Physiology Part A: Molecular &

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H 2 O Gluconeogenesis Glycogenolysis Glucose 6-phosphatase Glucokinase NMR Sources of blood glucose in European seabass (initial weight 218.0 ± 43.0 g; mean ± S.D., n = 18) were quantified by supplementing seawater with deuterated water (5%-2 H 2 O) for 72 h and analyzing blood glucose 2 H-enrichments by 2 H NMR. Three different nutritional states were studied: continuously fed, 21-day of fast and 21-day fast followed by 3 days of refeeding. Plasma glucose levels (mM) were 10.7 ± 6.3 (fed), 4.8 ± 1.2 (fasted), and 9.3 ± 1.4 (refed) (means ± S.D., n = 6), showing poor glycemic control. For all conditions, 2 H-enrichment of glucose position 5 was equivalent to that of position 2 indicating that blood glucose appearance from endogenous glucose 6-phosphate (G6P) was derived by gluconeogenesis. G6P-derived glucose accounted for 65 ± 7% and 44 ± 10% of blood glucose appearance in fed and refed fish, respectively, with the unlabeled fraction assumed to be derived from dietary carbohydrate (35 ± 7% and 56 ± 10%, respectively). For 21-day fasted fish, blood glucose appearance also had significant contributions from unlabeled glucose (52 ± 16%) despite the unavailability of dietary carbohydrates. To assess the role of hepatic enzymes in glycemic control, activity and mRNA levels of hepatic glucokinase (GK) and glucose 6-phosphatase (G6Pase) were assessed. Both G6Pase activity and expression declined with fasting indicating the absence of a classical counter-regulatory stimulation of hepatic glucose production in response to declining glucose levels. GK activities were basal during fed and fasted conditions, but were strongly stimulated by refeeding. Overall, hepatic G6Pase and GK showed limited capacity in regulating glucose levels between feeding and fasting states.

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“…Nesses estudos existem muitas variações em relação a esses nutrientes. Em trabalho realizado por Fernández et al (2007) Médias seguidas por letra distinta diferem entre si pelo teste Tukey a 5%. 1-PMI: Peso médio inicial; 2-PMF: Peso médio final; 3-Ganho de peso médio total; 4-GMD: Ganho médio diário; 5-CMRA: Consumo médio de ração aparente; 6-TCE: Taxa de crescimento específico 7-CAA: Conversão alimentar aparente; 8-GV: Gordura visceral; 9-RC: Rendimento de carcaça; 10-GL: Glicemia; 11-GH: Glicogênio hepático; 12-AAT: Aminoácidos totais livres.…”

Section: Resultsunclassified

“…1E). Ao testarem diferentes relações PB:CHO na dieta da dourada Sparus aurata, Fernández et al (2007) O rendimento da carcaça e gordura visceral nas relações 1,24; 0,84 e 0,51 não apresentaram diferença significativa (Tab. 2).…”

Section: Resultsunclassified

Relação proteína:carboidrato no desempenho e metabolismo de juvenis de pacamã (Lophiosilurus alexandri)

Figueiredo¹,

Souza

Bezerra

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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 RESUMOObjetivou-se neste estudo verificar o melhor nível de inclusão entre proteína e carboidrato no desempenho e algumas respostas metabólicas em juvenis de pacamã (Lophiosilurus alexandri). Testaramse quatro dietas com as relações proteína/carboidrato (PB:CHO) 1,24; 0,84; 0,51 e 0,33 durante 60 dias. Foram utilizados 240 juvenis de pacamã pesando inicialmente 1,90g, alimentados duas vezes ao dia (8% da biomassa). Avaliou-se o ganho de peso total, taxa de crescimento específico, consumo de ração aparente, conversão alimentar aparente, rendimento de carcaça e gordura visceral. Glicemia, aminoácidos totais livres no plasma e glicogênio hepático também foram avaliados. A relação PB:CHO influenciou (P<0,05) os parâmetros de desempenho e metabolismo, exceto o glicogênio hepático. Maiores concentrações de glicose e aminoácidos totais livres no plasma foram observadas nos peixes que se alimentaram com a dieta contendo a maior relação PB:CHO (1,24). Além disso, observaram-se melhores médias de ganho de peso, taxa de crescimento específico e conversão alimentar aparente. Maiores percentuais de gordura visceral e menor rendimento de carcaça foram observados nos animais submetidos à dieta composta pela menor relação PB:CHO (0,33). Pode-se concluir que a dieta com a relação PB:CHO de 1,24 promoveu melhor desempenho e respostas metabólicas em juvenis de pacamã.Palavras-chave: nutrição de peixes, respostas bioquímicas, bagre, pacamã ABSTRACT The aim of this study was to determine the best level of inclusion between protein and carbohydrate on performance and some metabolic responses in juvenile pacamã (Lophiosilurus alexandri

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Effects of diets with distinct protein-to-carbohydrate ratios on nutrient digestibility, growth performance, body composition and liver intermediary enzyme activities in gilthead sea bream (Sparus aurata, L.) fingerlings

Cited by 132 publications

References 41 publications

Glycolysis and gluconeogenesis in the liver of catfish fed with different concentrations of proteins, lipids and carbohydrates

Glycolysis and gluconeogenesis in the liver of catfish fed with different concentrations of proteins, lipids and carbohydrates

Effects of food-deprivation and refeeding on the regulation and sources of blood glucose appearance in European seabass (Dicentrarchus labrax L.)

Relação proteína:carboidrato no desempenho e metabolismo de juvenis de pacamã (Lophiosilurus alexandri)

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