Effects of diets rich in saturated fatty acids with or without added cholesterol on plasma lipids and lipoproteins

Abstract

1presented in part at the AOCS Annual Meeting, New Orleans, April 1970. dominantly of saturated medium chain length fatty acids, with or without added cholesterol were equally effective in the initiation of hyperlipemia. Data also suggest that added cholesterol is necessary for sustaining hyperlipemia.

Lipid metabolism of acetate‐1‐14C by leukocytes from dogs fed an arteriosclerosis‐inducing diet

“…Changes in the metabolism of acetate-l-14C by leukocytes isolated from dogs on atherogenic Diet I seemed to arise relatively rapidly and probably coincided with the rapidly changing plasma lipid pattern described elsewhere (5). Incubations carried out after animals had been on the diet 2-16 months gave similar results and did not appear to be related to length of time on either diet.…”

“…Adult male mongrel dogs weighing 12-20 kg were maintained on control ration (5) or a semisynthetic diet lacking essential fatty acids (Diet I), which has been shown to induce arteriosclerosis when fed for 1 year 05 longer (5)(6)(7). The composition and fatty acid distribution of Diet I, which contained 5% cholesterol and 16% hydrogenated coconut oil as the only source of lipid, was described previously (5). Blood leukocytes were isolated from dogs kept on these diets for 2-16 months.…”

“…Fatty acid patterns in isolated and washed leukocytes which were not mixed with plasma or isotope are given in Table V. In spite of the altered plasma fatty acid composition (5) and the lipid vacuolization observed in some WBC as described below, no differences were noted in the neutral lipid fatty acids. Only in phospholipids were there significant decreases in linoleic and arachidonic acids and an increase in the eicosatrienoic acids characteristic of essential fatty acid deficiency.…”