Effects of dietary zinc oxide and selenium nanoparticles on growth performance, immune responses and enzyme activity in rohu,Labeo rohita(Hamilton)

Abstract

Zinc is the second most abundant trace element in the animal body.It cannot be stored in the body (Zalewski et al., 2005) and can be consumed as regular dietary intake to meet the physiological need of the body. As a component of numerous enzymes and hormones, it is necessary for the proper physiological functioning (Prasad, 1991).Several important enzymatic activities like alcohol dehydrogenase, alkaline phosphatase (ALP), aldolase, lactate dehydrogenase (LDH), RNA and DNA polymerases, reverse transcriptase and superoxide dismutase (SOD) are dependent on this micronutrient. Zinc oxide nanoparticles have been stated to enhance growth performance, Abstract A 120-day feeding trial was conducted to assess the effects of dietary supplementation of zinc oxide (ZnO) and selenium (Se) nanoparticles (NPs) on growth, immunological and enzymatic profiles in rohu, Labeo rohita. Fishes were fed with basal diet incorporated with laboratory-synthesized nanoparticles, viz., zinc oxide nanoparticles-10 mg/kg and Se nanoparticles-0.3 mg/kg throughout the experimental period. At an interval of every 30 days, treated and control fishes were randomly collected and subjected to estimation of growth, non-specific immune parameters and serum enzyme assays. It was observed that there was significant (p < 0.05) increase in growth and non-specific immune parameters like respiratory burst, lysozyme and myeloperoxidase activities as compared to the control group (only basal diet). Serum enzymatic profiles such as lactate dehydrogenase and alkaline phosphatase activities down-regulated (p < 0.05) in treated group compared to control group, and superoxide dismutase and acetylcholine esterase activity up-regulated in treated group. The relative percentage survival (RPS) found to be significantly higher in treated groups (60.00 ± 8.82%) as compared to control group (45.00 ± 6.17%) while challenged with virulent bacterial strain Aeromonas hydrophila. These results indicated that dietary supplementation of zinc oxide (ZnO) and selenium (Se) nanoparticles stimulates immunity and enhances resistance to bacterial infection in L. rohita. K E Y W O R D SA. hydrophila, aquaculture, enzyme activities, growth performance, immune response, nanoparticles How to cite this article: Swain P, Das R, Das A, Padhi SK, Das KC, Mishra SS. Effects of dietary zinc oxide and selenium nanoparticles on growth performance, immune responses and enzyme activity in rohu, Labeo rohita (Hamilton). Aquacult Nutr. 2019;25:486-494. https://doi.

Study on the impacts of chemical and green synthesized (Leucas aspera and oxy-cyclodextrin complex) dietary zinc oxide nanoparticles in Nile tilapia (Oreochromis niloticus)

“…This suggests that the neutrophils were stimulated to release lysozyme in the presence of nanoparticles. Similarly, dietary zinc oxide and selenium nanoparticle feed were revealed to enhance the lysozyme activity in Labeo rohita (Swain et al 2018), β-1,3 glucan binding protein-based zinc oxide nanoparticle elevated lysozyme activity (Anjugam et al 2018) which are in line with the present investigation. Only a few studies have been reported so far that determined the lysozyme activity in fish after treating it with nanoparticles.…”

Evaluation of oxytetracycline hydrochloride (OTC) on non‐specific immunity, enzymatic profiles and tissue level retention in Labeo rohita (Hamilton, 1822) after administration through feed

“…Serum was separated from the blood (without EDTA) by centrifuging at 2000 g for 15 min at 4°C' (Swain et al, 2019).…”

Effect of Solid-state Fermented Aquafeed on Growth Performance, Digestive Enzymes and Innate Immunity of Rohu, Labeo rohita

“…The total fish biomass per pond was measured at the beginning of the feeding trial and subsequently at the end of the trial Blood collection was done at the end of the experimental trial by randomly selecting 10 fishes from each pond. Blood and serum samples of fish were used for laboratory analyses (Swain et al, 2018).…”

“…Bacterial agglutination test was carried out in "U" shaped microtitre plates as the method described by Swain et al (2018). A 25 μl of fish serum sample was two fold serially diluted and added in each well with an equal volume of normal saline solution (NSS).…”