Effects of dietary thiamin on the physiological status of the grouper Epinephelus coioides

Abstract

This study was conducted to evaluate the effects of dietary thiamin on the physiological status of the juvenile grouper, Epinephelus coioides. Graded levels of thiamin (0.08, 0.50, 2.12, 3.15, 4.63, 12.37 mg thiamin kg -1 diet) were fed to grouper juveniles (mean weight: 16.97 ± 0.14 g) for 10 weeks. Although fish fed the thiamin-deficient (TD) diet showed no obvious symptoms of thiamin deficiency or increased mortality, those fed the lowest doses of thiamin (0.08 and 0.50 mg thiamin kg -1 diet) had significantly decreased transketolase activity in the liver. In addition, the level of liver thiobarbituric acid reactive substances in fish fed the TD diet was 33-67% higher than that in fish with the thiamin-supplemented diet. There were no significant differences in superoxide dismutase activity between the different groups of fish.

Dietary thiamin could improve growth performance, feed utilization and non-specific immune response for juvenile Pacific white shrimp (Litopenaeus vannamei)

“…However, Huang et al . () found that there was no significant difference in SOD activity in the liver. Therefore, further research on the relationship between antioxidation and dietary thiamin levels needs to be carried out.…”

“…The coenzyme form of thiamin (the pyrophosphate form) acts as an important coenzyme for transketolase and several other enzymes involved in the conversion of carbohydrates and fat into energy, including pyruvate dehydrogenase and a‐ketoglutarate dehydrogenase, which are important in carbohydrate metabolism (Huang et al . ). In the present study, the whole‐body dry matter and protein contents of the shrimp were significantly influenced by dietary thiamin levels.…”

Evaluation the effect of thiamin deficiency on intestinal immunity of young grass carp (Ctenopharyngodon idella)

“…After a four-week adaption period as described by Lin et al [36], grass carp were fed with the thiamin-deficient diet for 2 weeks to reduce the body storage of thiamin according to Huang et al [37]. At the beginning of the experiment, a total of 540 young grass carp with an initial weight of 243.24 ± 1.27 g (average body length of 24.07 ± 1.86 cm) were randomly distributed into 18 experimental cages (1.4 Â 1.4 Â 1.4 m 3 ) and resulted in 30 fish in each cage.…”

Effects of dietary riboflavin levels on antioxidant defense of the juvenile grouper Epinephelus coioides

“…However, most studies on oxidative stress in fish have focused on natural and anthropogenic toxicological aspects, such as intrinsic factors in the fish itself, environmental factors and xenobiotics (Winston and Di Giulio 1991). Recent research has begun to pay attention to the roles of nutrient factors in the antioxidant system of fish, and a few nutrients have been preliminarily studied, including energy diets such as protein (Lopez-Bote et al 2001), lipids (Alvarez et al 1998), fatty acids (Stéphan et al 1995) and digestible carbohydrate (Rueda-Jassoa et al 2004); vitamins such as vitamin E (George et al 2000), vitamin C (Dabrowski et al 2004), vitamin A (Fontagné et al 2006), pyridoxine (Huang et al 2005) and thiamin (Huang et al 2007); minerals such as selenium (Lin and Shiau 2005), iron (Andersen et al 1998) and zinc (Kucukbay et al 2006). However, little research (but see Woodward 1983) concerns the role of riboflavin in antioxidant defense systems in fish.…”

“…Recently in our laboratory, some of the B-family vitamins, such as pyridoxine (Huang et al 2005) and thiamin (Huang et al 2007), have been studied concerning their physiological function as well as antioxidant role in this fish species. Therefore, as one of the studies among a series of research focusing on the physiological and biochemical function of the B-family vitamins in E. coioides, the aim of the present study was to provide more precise information concerning the effects of dietary riboflavin on antioxidant defense.…”