Effects of dietary sweeteners supplementation on growth performance, serum biochemicals, and jejunal physiological functions of broiler chickens

Abstract

The objective of this study was to investigate the effects of dietary 3 kinds of sweeteners supplementation on growth performance, serum biochemicals, and jejunal physiological functions of broiler chickens for 21 D. A total of one hundred ninety-two 1-day-old male Ross 308 broiler chicks were randomly divided into 4 treatments with 6 replicates for each treatment. The treatments were basal diet ( CON ), a basal diet supplemented with 250 mg/kg stevioside ( STE ), a basal diet supplemented with 100 mg/kg sucralose ( SUC ), and a basal diet supplemented with 600 mg/kg saccharin sodium ( SAC ). All birds were housed in 3-level battery cages. The results showed that dietary STE supplementation increased ( P < 0.05) growth performance, serum total protein, serum albumin, and jejunal antioxidant capacity of broiler chickens. Both SUC and SAC supplementation decreased ( P < 0.05) serum total protein and albumin. Dietary SAC supplementation impaired the intestinal integrity, permeability, and mucus layer of the jejunum in broiler chickens. In addition, SAC supplementation elevated ( P < 0.05) the transcription expression level of jejunal bitter taste receptors and induced excessive jejunal apoptosis. Our data suggest that STE could be potentially applied as a growth-promoting and antioxidant feed additive in broiler chickens. Whereas, dietary supplementation with high level SAC has side-effects on the jejunal physiological functions of broiler chickens.

Impact of Stevioside Supplementation as Feed Additive in Finisher Broiler Diets on Growth Performance, Carcass Traits, Meat Quality, Selected Biochemical Parameters, and Caecum Microflora

“…The increase in total protein and globulin with STE addition may be due to increased protein synthesis. The obtained results were supported by Jiang et al (2020), who found that the addition of STE at 250 mg/kg broilers diet significantly increased total protein concentration. These findings, on the other hand, are in contrast to those of Wu et al (2019), who found that STE supplementation had no effect on total serum protein.…”

“…Results of GP coincided with the results reported by Atteh et al (2008), who concluded that STE addition enhanced the average BWG of broiler chicks. Also, Jiang et al (2020) mentioned that dietary addition with STE increased the GP of broiler chicks. Inversely, Wu et al (2019) found that STE addition to 3200 mg/ kg diet did not affect GP of broilers through a 42d study.…”

Dietary stevioside supplementation increases feed intake by altering the hypothalamic transcriptome profile and gut microbiota in broiler chickens

“…Nevertheless, owing to the lack of taste receptor family 1 member 2, chickens are insensitive to sweet substances 3 . Our previous study demonstrated that STE could not activate the intestinal taste receptors in broiler chickens 4 . However, our present data of increased cumulative feed intake in broiler chickens reveals that STE might regulate feeding behavior through other functional mechanisms than its high‐potency sweetness.…”

“…This makes the chicken an ideal animal model for the study of whether sweeteners could regulate the appetite through a sweetness‐independent mechanism. Our recent study has demonstrated that dietary STE supplementation could increase the feed intake of broiler chickens at an early age, whereas supplementation with other high‐potency sweetener (sucralose or saccharin sodium) has no such effects 4 . Investigation on its potential mechanisms could provide us with a new perspective in understanding the function of STE on the feeding behavior of animals.…”

L-Citrulline Influences the Body Temperature, Heat Shock Response and Nitric Oxide Regeneration of Broilers Under Thermoneutral and Heat Stress Condition

“…The temperature and relative humidity of the chambers were monitored continuously throughout the experiment. Blood was collected from the wing vein of individual birds into heparinized and non-heparinized tubes, and centrifuged at 3,000 × g for 10 min at 4°C to obtain plasma and serum samples respectively ( Jiang et al, 2020 ; Guo et al, 2021 ). Samples were stored at −20°C until analysis.…”