Effects of dietary supplementation with vitamin E and selenium on rat hepatic stellate cell apoptosis

Shen, Xiaotian; Wu, Cheng; Li, Xuan Hai; Sun, Jing; Li, Feng; Ma, Lu; Xie, Liang

doi:10.3748/wjg.v11.i32.4957

Cited by 22 publications

(14 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In previous in vivo studies, dietary supplementation of Se in mice [13] or vitamin E in rats [14] decreased hepatic fibrosis caused by CCl 4 administration. In rats, administration of Se with vitamin E decreased fibrosis produced by chronic CCl 4 administration [15]. Vitamin E supplementation alone was shown to inhibit α 1 (I) collagen gene expression in rats, as well as the transactivation of the human α 1 (I) collagen in isolated stellate cells obtained from transgenic mice bearing this collagen gene [20].…”

Section: Discussionmentioning

confidence: 98%

“…In another study, administration of vitamin E decreased serum hyaluronic acid levels [10], which correlates with stages of hepatic fibrosis in liver disease [11,12]. Hepatic fibrosis after CCl 4 administration was decreased in mice by dietary supplementation of Se [13] or in rats by vitamin E [14] or the combination of vitamin E and Se [15]. A combination of decreased formation and increased degradation of collagen was considered as a cause of the lesser accumulation of collagen in the liver of the Se-supplemented mice [13].…”

mentioning

confidence: 98%

See 1 more Smart Citation

The Combination of Selenium and Vitamin E Inhibits Type I Collagen Formation in Cultured Hepatic Stellate Cells

Mezey

Liu

Potter

2010

Biol Trace Elem Res

View full text Add to dashboard Cite

This study investigated the effects of sodium selenite (Se) and of vitamin E (D-α-tochopherol) on the deposition of type I collagen by human LX-2 stellate cells. The cultured cells were treated with or without Se or vitamin E and with or without transforming growth factor β1 (TGFβ1). The combination of Se and vitamin E, but not either alone, protected against hepatic fibrosis by decreasing TGFβ1-mediated collagen secretion and accumulation by the stellate cells. This protective effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen. Effects of Se and vitamin E in decreasing α(1)(I) collagen mRNA and increasing apoptosis of stellate cells indicate decreased formation of collagen, while decreases in transglutaminase 2, which catalyze cross-linking of collagen, lead to decreased stability of the secreted collagen. Effects of Se and vitamin E on reducing tissue inhibitor metalloproteinase 1 (TIMP-1) are associated with increased degradation. The combination of Se and vitamin E decreased lipid peroxidation, while Se alone increased the activity of the antioxidant enzyme thioredoxin reductase. In conclusion, the combination of Se and vitamin E protected against TGFβ1-mediated hepatic fibrosis by decreasing TGFβ1-mediated type I collagen accumulation by stellate cells. This effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen.

show abstract

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 98%

The Combination of Selenium and Vitamin E Inhibits Type I Collagen Formation in Cultured Hepatic Stellate Cells

Mezey

Liu

Potter

2010

Biol Trace Elem Res

View full text Add to dashboard Cite

show abstract

“…The reduction in hepatic fibrosis without improvement in necroinflammation needs to be clarified. One possible explanation may be the effect of vitamin E on rat hepatic stellate cell in inducing apoptosis and thus reducing extracellular collagen accumulation [29] . Therefore, in our study, the decrease in hepatic fibrosis in rats taking vitamin E may result from reduced hepatic stellate cell numbers.…”

Section: Discussionmentioning

confidence: 99%

Antioxidants vitamin E and C attenuate hepatic fibrosis in biliary-obstructed rats

Soylu

Aydoğdu²,

Başaran³

et al. 2006

WJG

View full text Add to dashboard Cite

AIM:To investigate whether antioxidants vitamin E and C can retard development of hepatic fibrosis in the biliary-obstructed rats. METHODS:Fifty Wistar albino rats were randomly assigned to 5 groups (10 rats in each). Bile duct was ligated in 40 rats and they were treated as follows: group vitC, vitamin C 10 mg/kg sc daily; group vitE, vitamin E 15 mg/kg sc daily; group vitEC, both of the vitamins; bile duct-ligated (BDL, control) group, physiological saline sc. The fifth group was assigned to sham operation. At the end of fourth week, the rats were decapitated, and hepatic tissue biochemical collagen content and collagen surface area were measured. Hepatic tissue specimens were histopathologically evaluated according to Scheuer system. Serum hyaluronate levels were measured by ELISA method. RESULTS:Despite being higher than sham group, hepatic collagen level was significantly decreased in each of the vitC, vitE and vitEC groups (32.7 ± 1.2, 33.8 ± 2.9, 36.7 ± 0.5 μg collagen/mg protein, respectively) compared to BDL (48.3 ± 0.6 mg collagen/g protein) (P < 0.001 for each vitamin group). Each isolated vitamin C, isolated vitamin E and combined vitamin E/C supplementation prevented the increase in hepatic collagen surface density (7.0% ± 1.1%, 6.2% ± 1.7%, 12.3% ± 2.0%, respectively) compared to BDL (17.4% ± 5.6%) (P < 0.05 for each). The same beneficial effect of vitamin C, vitamin E and combined vitamin E/C treatment was also observed on the decrease of serum hyaluronate levels compared to BDL group (P < 0.001). The relative liver and spleen weights, serum transaminases, cholestatic enzymes, bilirubins and histopathological inflammation scores were not different between the antioxidant treatment groups and the control. However, fibrosis staging scores were obviously reduced only in the vitamin E/C combination group (vit EC: 2.4 ± 0.8 vs BDL: 3.1 ± 0.7; P < 0.05). CONCLUSION:Each antioxidant vitamin E, vitamin C and their combination retard hepatic fibrosis in biliary-obstructed rats. Oxidative stress may play a role in the pathogenesis of hepatic fibrosis in secondary biliary cirrhosis.

show abstract

“…In other studies, however, administration of Se together with vitamins A and E did not affect survival of patients with alcoholic hepatitis [11]. Selenium-enriched lactobacillus decreased liver injury and lipid peroxidation induced by CCl 4 administration in mice [12], while supplementation of the diet with vitamin E and Se decreased hepatic fibrosis produced in rats by acute and chronic CCl 4 administration [13]. …”

Section: Introductionmentioning

confidence: 99%

Selenium Supplementation Decreases Hepatic Fibrosis in Mice After Chronic Carbon Tetrachloride Administration

Ding

Potter

Liu

et al. 2009

Biol Trace Elem Res

View full text Add to dashboard Cite

Oxidative stress stimulates fibrogenesis, and selenium (Se) has antioxidant properties. This study determined whether Se supplementation affects CCl4-induced liver injury and fibrosis. Mice were administered CCl4 over 4 weeks, while controls received olive oil. Se was provided as sodium selenite in the drinking water. Se increased liver Se-dependent glutathione peroxidase activity and decreased liver malondialdehyde after CCl4. Se decreased liver inflammation but not necrosis caused by CCl4. Se increased hepatocyte apoptosis after CCl4 and the pro-apoptotic BAX and Bcl Xs/l proteins. Stellate cell apoptosis occurred only after CCl4 in Se-supplemented mice. Se decreased stellate cell number and fibrosis after CCl4. Liver matrix metalloproteinase-9 increased after CCl4 with Se supplementation. In conclusion, Se supplementation decreased hepatic fibrosis after CCl4 in the setting of decreased inflammation but increased apoptosis. The principal mechanisms for the decreased fibrosis are a lower number of collagen-producing stellate cells and increased collagen degradation.

show abstract

Effects of dietary supplementation with vitamin E and selenium on rat hepatic stellate cell apoptosis

Cited by 22 publications

References 12 publications

The Combination of Selenium and Vitamin E Inhibits Type I Collagen Formation in Cultured Hepatic Stellate Cells

The Combination of Selenium and Vitamin E Inhibits Type I Collagen Formation in Cultured Hepatic Stellate Cells

Antioxidants vitamin E and C attenuate hepatic fibrosis in biliary-obstructed rats

Selenium Supplementation Decreases Hepatic Fibrosis in Mice After Chronic Carbon Tetrachloride Administration

Contact Info

Product

Resources

About