Effects of dietary supplementation of Artemisia argyi aqueous extract on antioxidant indexes of small intestine in broilers

Abstract

The present study was conducted to investigate the effect of Artemisia argyi aqueous extract (AAE) on antioxidant indexes in the small intestine. A total of 192 Arbor Acre broiler chickens (one-day-old) were randomly divided into 4 treatments with 6 replicates of 8 chickens. These 4 diets were formulated by adding 0, 500, 1,000 and 2,000 mg/kg AAE to the basal diet. The results showed as follows: 1) compared with the control, the total antioxidant capacity (T-AOC) in ileum for the 2,000 mg/kg treatment group was significantly increased at 21 days of age (P < 0.05); the T-AOC levels in jejunum and ileum were significantly increased in broilers supplemented with 500 mg/kg AAE at 42 days of age (P < 0.05), and the T-AOC levels in jejunum and ileum were significantly improved in 1,000 mg/kg treatment group (P < 0.01). 2) At 21 days of age, supplementation of 500 mg/kg AAE significantly increased the catalase (CAT) activity of small intestine, and the glutathione peroxidase (GSH-Px) activity of jejunum was improved (P < 0.01), meanwhile, the GSH-Px activity of duodenum and the total superoxide dismutase (T-SOD) activity of duodenum and jejunum were significantly higher than those of the control group (P < 0.05); supplementation of 1,000 mg/kg AAE significantly increased the CAT activity of duodenum and ileum and the GSH-Px activity of duodenum and jejunum (P < 0.05), and the ileum GSH-Px activity was significantly increased (P < 0.01); supplementation of 2,000 mg/kg AAE significantly increased the CAT activity of duodenum and ileum (P < 0.05). At 42 days of age, supplementation of 500 mg/kg AAE significantly increased the GSH-Px activity of ileum and the T-SOD activity of duodenum (P < 0.05), meanwhile, the T-SOD activity of jejunum was significantly increased (P < 0.01); supplementation of 1,000 mg/kg AAE significantly increased the CAT activity of jejunum and the T-SOD activity of ileum (P < 0.01), and the GSH-Px activity of jejunum was significantly increased (P < 0.05); supplementation of 2,000 mg/kg AAE significantly increased the T-SOD activity of ileum (P < 0.05), but significantly decreased the CAT activity of ileum and the GSH-Px activity of jejunum (P < 0.05). 3) The malondialdehyde (MDA) levels of 3 AAE supplementation groups were significantly decreased at 21 and 42 days of age (P < 0.05). The results suggested that dietary supplementation with AAE could improve the antioxidative capacity of small intestine in broilers.

Dietary supplementation with Artemisia argyi extract on inflammatory mediators and antioxidant capacity in broilers challenged with lipopolysaccharide

“…However, AAE supplementation tended to increase the levels of serum GSH-Px (p¼ .09) and CAT (p¼ .06), and feeding AAE to the LPS-challenged groups increased the expression of jejunal CAT and GPx7, as well as the gene expression of ileac GPx7 (p < .05). In addition, AAE, as a main effect, reduced serum MDA content (p¼ .02), which may be the result of free-radical-scavenging capacity of AAE reported by Zhao et al (2016), or the improvement of serum CAT and GSH-Px content observed in this study. Therefore, combining the experiments in vivo and in vitro, it could reveal the remarkable suppressive effect of AAE on oxidative stress induced by LPS.…”

Chemical Composition and Antioxidant Activities of Polysaccharides from Yingshan Cloud Mist Tea

“…Total antioxidant capacity (T-AOC) was a main index to decide the total antioxidant level of the enzymatic and nonenzymatic system. T-AOC can eliminate free radicals and reactive oxygen species and further inhibit lipid peroxidation, reduce peroxidation materials, and remove metal ions from the catalytic reaction [39]. Malondialdehyde (MDA) is a main animal body product of lipid peroxidation.…”

Algae-Derived Polysaccharides Promote Growth Performance by Improving Antioxidant Capacity and Intestinal Barrier Function in Broiler Chickens

“…For instance, the inclusion of 30 or 350 mg/kg COS in broilers' diet was reported to improve the intestinal mucosa oxidative damage by enhancing SOD and GSH-Px enzyme activities and reducing the content of MDA (22,42). The Artemisia argyi aqueous extract that contains an abundant amount of natural polysaccharides, feeding to broilers at 500-2,000 mg/kg level, increased the total antioxidant capacity, SOD, and GSH-Px activities by decreasing the MDA levels in small intestinal tissues (43). Thus, the natural polysaccharides can act as a defensive mechanism for antioxidant status in broilers' intestinal mucosa and can prevent the uncontrolled formation of ROS and/or directly scavenging the free radicals.…”