Dietary supplementation with Artemisia argyi extract on inflammatory mediators and antioxidant capacity in broilers challenged with lipopolysaccharide

Abstract

This study investigated the effects of Artemisia argyi extract (AAE) on broilers challenged with lipopolysaccharide (LPS). A total of 96 Arbour Acres broilers (1-d-old) were assigned to a 2 Â 2 factorial arrangement with two dietary treatments (AAE at 0 or 1000 mg/kg) and two immunological challenge treatments (saline or LPS). On d 14, 16, 18 and 20, the broilers were injected intra-abdominally with LPS solution at 500 lg/kg of body weight or an equivalent amount of sterile saline. Blood, liver, spleen and small intestine were collected on day 21. The increased relative weights of the thymus and spleen induced by LPS were significantly decreased by AAE supplementation. The level of serum nitric oxide (NO) and the activity of inducible NO synthase (iNOS) were significantly compromised by AAE inclusion. Dietary AAE significantly inhibited the mRNA expression of toll-like receptor 4 (TLR4), myeloid differentiation factor 88, nuclear factorkappa B (NF-jB) and iNOS in the different tissues of LPS-challenged broilers. The AAE supplementation tended to increase the levels of serum GSH-Px and CAT, but significantly reduced the level of serum malondialdehyde (MDA). Collectively, feeding AAE to LPS-challenged broilers could decrease serum NO level, maintain the relative weight of internal organs, enhance the antioxidant capacity, and inhibit TLR4/NF-jB signalling pathway at the transcriptional level. HIGHLIGHTS AAE inclusion significantly inhibited the serum NO level, and the activity of iNOS induced by LPS. AAE supplementation tended to increase the levels of serum GSH-Px and CAT, but significantly reduced the level of serum MDA. Feeding AAE to the LPS-challenged broilers reduced the expression of TLR4, MyD88 and NF-jB in the liver, spleen and small intestine.

The influence of immune stress induced by Escherichia coli lipopolysaccharide on the pharmacokinetics of danofloxacin in broilers

“…These increases might be because that LPS, as a nonspecific immunogen, could induce immune stress in animals and damage immune organs. The current results in the spleen indices were consistent with the previous ones ( Li et al, 2015 ; Liu et al, 2015 ; Zhang et al, 2020 ). As an essential lymph organ, the spleen can activate lymphocytes after LPS injections, further increasing the number of lymph nodes in the white pulp, the inflammatory cytokines, and the activity of the organ.…”

Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

“…According to the method reported by De Oliveira and Lara (31), the chicken houses were illuminated by LED lights that provide 30-40 lux of light intensity. The lighting scheme included 23 h lighting (L):1 h darkness (D) (23L:1D, days 0-3), 10 L:14 D (days 4-21), 14 L:10 D (days 22-28), 18 L:6 D (days [29][30][31][32][33][34][35], and 23 L:1 D (days 36-42). The temperature of the experimental room was set at 32-34 • C for the first 3 days and then gradually reduced by 3 • C every week, and a final temperature of 21 • C was reached.…”

“…Furthermore, aqueous and alcoholic extracts of A. annua improved insulin resistance via decreasing TNFalpha and IL-6 in high-fat diet/streptozotocin-induced diabetic mice (27). Our previous study found that Artemisia argyi and Artemisia ordosica aqueous extract could improve the immune and antioxidant status of LPS-induced broilers (28,29). Given these features, the present study used water as a solvent to extract the bioactive components of A. annua, and aimed to investigate the effects of A. annua aqueous extract on intestinal immune function, and antioxidant capability in broilers.…”

Dietary chitooligosaccharide supplementation alleviates intestinal barrier damage, and oxidative and immunological stress in lipopolysaccharide-challenged laying hens

“…According to the substantial papers, LPS challenge can induce immunological and oxidative stress, and severely impair the intestinal morphology and permeability, and it would interfere with metabolic process and organ functions, contributing to the inferior productive performance of laying hens ( Jing et al, 2014 ; Geng et al, 2018 ; Liu et al, 2020 ). In commercial practice, dietary intervention can be a promising and potential method to resist against the stress responses induced by LPS or other stimuli for improving the performance of poultry and other livestock ( Pi et al, 2014 ; Zhang et al, 2020 ).…”