Effects of Dietary Selenium Supplementation on Intestinal Barrier and Immune Responses Associated with Its Modulation of Gut Microbiota

Zhai, Qixiao; Cen, Shi; P, Li; Tian, Fengwei; Zhang, Hao

doi:10.1021/acs.estlett.8b00563

Cited by 99 publications

(84 citation statements)

References 39 publications

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“…Total RNA was extracted with Trizol reagent and converted to cDNA using Taq-Man reverse transcription reagents (Applied Biosystems; Foster City, CA, United States). Real-time PCR parameters and primer sequences for the targeted mouse genes were as previously described (Zhai et al, 2018).…”

Section: The Evaluation Of Tj Protein Mrna Expression In Intestinal Tmentioning

confidence: 99%

Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice

Zhai

Feng

et al. 2020

Front. Microbiol.

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Oral exposure to the heavy metal lead (Pb) causes various dysfunctions in animals. However, the influence of gut bacteria on Pb absorption, bioaccumulation, and excretion is largely unknown. In this study, we use a mouse model to investigate the relationship between gut microbiota, Pb-intolerant intestinal microbes and Pb toxicity. First, mice were treated with a broad-spectrum antibiotic cocktail to deplete their gut microbiota, and were then acutely and orally exposed to Pb at 1304 mg/kg for 3 days. Compared to the control mice, antibiotic-treated mice had increased Pb concentrations in the blood and primary organs and decreased Pb fecal concentrations, suggesting that gut microbiota limited the Pb burden that developed from acute oral Pb exposure. Next, three Pb-intolerant gut microbes, Akkermansia muciniphila, Faecalibacterium prausnitzii, and Oscillibacter ruminantium, were orally administered to mice, and their effects against Pb toxicity were evaluated. F. prausnitzii treatment significantly promoted the fecal Pb excretion and reduced Pb concentrations in blood (from 152.70 ± 25.62 µg/dL to 92.20 ± 24.33 µg/dL) and primary tissues. Supplementation with O. ruminantium significantly decreased Pb concentrations in blood (from 152.70 ± 25.62 µg/dL to 104.60 ± 29.85 µg/dL) and kidney (from 7.30 ± 1.08 µg/g to 5.64 ± 0.79 µg/g). Treatment with F. prausnitzii and O. ruminantium also upregulated tight junction (TJ) protein expression and the production of short-chain fatty acids by colonic microbiota, and showed protective effects against liver and kidney toxicity. These results indicate the potential for reducing Pb toxicity by the modulation of gut microbiota.

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Section: The Evaluation Of Tj Protein Mrna Expression In Intestinal Tmentioning

confidence: 99%

Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice

Zhai

Feng

et al. 2020

Front. Microbiol.

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“…The ability of selenium to enhance immunity is not just determined by its direct action on the host, but also through its effects in the microbiome, that will increase or decrease the susceptibility to infections provoked by specific microorganisms (Zhai et al . 2018 ). A limited number of detailed studies have evaluated the effect of selenium supplementation on the gut microbiome of fish (Kousha, Yeganeh and Amirkolaie 2019 ; Victor et al .…”

Section: Introductionmentioning

confidence: 99%

“… 2015 ; Zhai et al . 2018 ). In summary, these studies point towards a positive impact of selenium supplementation on bacteria diversity (Victor et al .…”

Section: Introductionmentioning

confidence: 99%

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Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies

Pereira

Pinna

Biagi

et al. 2020

FEMS Microbiology Ecology

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Selenium is an essential trace element that can modulate the gut microbiome with an impact on host health. The present study aimed to evaluate the effects of organic (selenium-enriched yeast) vs inorganic (sodium selenite) selenium source on fecal end-fermentation products and gut microbiome of puppies from 20 to 52 weeks of age. Alpha and beta diversity of the gut bacterial community were affected by age but not by gender or selenium source. The relative abundance of taxa was differently affected by age, and the DNA concentration of all selected bacterial groups increased with age, although total volatile fatty acids (VFA), acetate, propionate, caproate, and lactate concentrations decreased. Organic selenium was associated with a higher concentration of total VFA, propionate, and butyrate, a higher number of DNA copies of Lactobacillus, and a trend to lower DNA copies of Escherichia coli. Effects on fecal microbiome during growth differed with selenium source. Females had higher fecal end-fermentation products related to protein degradation, whereas males had higher DNA concentration of Bifidobacterium. Organic selenium might be beneficial over inorganic for dog food supplementation due to the positive modulation of the gut microbiome observed in puppies.

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“…To date, no research has investigated these interactions in any insect species. Much less is known about the effects of selenium on gut microbial communities, but subtle alterations in these microbe populations have been revealed by previous studies performed under conditions of exposure to selenium ions in mice (45,46) and through our work with bumble bees (35). In light of the effects of toxicant exposure on the microbiome, research is now being conducted on the ability of this microbial community to protect its host against environmental exposures.…”

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confidence: 99%

Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

Rothman

Leger

Kirkwood

et al. 2019

Appl Environ Microbiol

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Honey bees are important insect pollinators used heavily in agriculture and can be found in diverse environments. Bees may encounter toxicants such as cadmium and selenate by foraging on plants growing in contaminated areas, which can result in negative health effects. Honey bees are known to have a simple and consistent microbiome that conveys many benefits to the host, and toxicant exposure may impact this symbiotic microbial community. We used 16S rRNA gene sequencing to assay the effects that sublethal cadmium and selenate treatments had over 7 days and found that both treatments significantly but subtly altered the composition of the bee microbiome. Next, we exposed bees to cadmium and selenate and then used untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics to show that chemical exposure changed the bees' metabolite profiles and that compounds which may be involved in detoxification, proteolysis, and lipolysis were more abundant in treatments. Finally, we exposed several strains of bee-associated bacteria in liquid culture and found that each strain removed cadmium from its medium but that only Lactobacillus Firm-5 microbes assimilated selenate, indicating the possibility that these microbes may reduce the metal and metalloid burden on their host. Overall, our report shows that metal and metalloid exposure can affect the honey bee microbiome and metabolome and that strains of bee-associated bacteria can bioaccumulate these toxicants. IMPORTANCE Bees are important insect pollinators that may encounter environmental pollution when foraging upon plants grown in contaminated areas. Despite the pervasiveness of pollution, little is known about the effects of these toxicants on honey bee metabolism and their symbiotic microbiomes. Here, we investigated the impact of selenate and cadmium exposure on the gut microbiome and metabolome of honey bees. We found that exposure to these chemicals subtly altered the overall composition of the bees' microbiome and metabolome and that exposure to toxicants may negatively impact both host and microbe. As the microbiome of animals can reduce mortality upon metal or metalloid challenge, we grew bee-associated bacteria in media spiked with selenate or cadmium. We show that some bacteria can remove these toxicants from their media in vitro and suggest that bacteria may reduce metal burden in their hosts.

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Effects of Dietary Selenium Supplementation on Intestinal Barrier and Immune Responses Associated with Its Modulation of Gut Microbiota

Cited by 99 publications

References 39 publications

Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice

Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice

Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies

Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

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