Effects of dietary saturated and polyunsaturated fat on lipoprotein lipase and hepatic triglyceride lipase activity

Coiffier, Eric; Paris, R; Lecerf, Jean

doi:10.1016/0305-0491(87)90099-x

Cited by 14 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results showing an increase in hepatic lipase mRNA on supplementation of the diet with saturated but not polyunsaturated fat (Fig. 4) are consistent with those of Coiffier et al [21]; we also recorded an increase with monounsaturated fat. These changes, however, did not correlate with the changes observed in the removal of chylomicron cholesterol from the blood in the rats fed low and high fat ( Fig.…”

Section: Discussionsupporting

confidence: 93%

“…Hepatic lipase is believed to have a role in the uptake of chylomicron remnants by the liver both by modification of the particles through its enzyme activity and by acting as a ligand for binding to the surface of the hepatocytes, while LCAT is a key enzyme in reverse cholesterol transport 1421. Coiffier et al [21) have shown that the activity of hepatic lipase is increased in rats fed high-fat diets, with saturated fat having a greater effect than polyunsaturated fat, but the influence of dietary fats on the hepatic expression of mRNA for these two important enzymes of lipoprotein metabolism has not been reported previously. Our results showing an increase in hepatic lipase mRNA on supplementation of the diet with saturated but not polyunsaturated fat (Fig.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of the Uptake and Processing of Cholesterol from Chylomicrons of Different Fatty Acid Composition in Rats Fed High‐Fat and Low‐Fat Diets

Bravo

Flora

Cantàfora

et al. 1997

European Journal of Biochemistry

View full text Add to dashboard Cite

The fate of [3H]cholesterol carried in chylomicrons prepared from rats given a meal of palm oil (rich in long-chain saturated fatty acids), olive oil (rich in monounsaturated fatty acids) or corn oil (rich in n-6 polyunsaturated fatty acids) was investigated in vivo in rats fed a low-fat diet or a diet supplemented with the corresponding oil (to provide 40% of the calories) for 21 days. In the low-fat-fed groups, radioactivity was removed from the blood and secreted into bile over 180 min more rapidly when the chylomicrons were derived from corn oil as compared to palm or olive oil. After feeding the corresponding high-fat diets, however, both parameters were decreased in rats fed palm and corn oil, but not olive oil. As a result of these changes, the rates of removal of radioactivity from the blood and secretion into bile were similar in animals given the olive oil and corn oil diets, and higher than those in rats fed the palm oil diet. All the high-fat diets tended to increase the proportion of the radioactivity in the plasma found in the 1.006-1.050-g/ml fraction (low-density lipoprotein) and decrease that in the 1.050-1.25-g/ml (high-density lipoprotein) fraction in comparison to the respective low-fat diet groups, but the transfer of radioactivity to the plasma high-density lipoprotein fraction was particularly slow in palm-oilfed rats. These findings indicate that diets high in saturated or n-6 polyunsaturated fat retard the metabolism of chylomicron cholesterol in comparison to diets low in fat, while those high in monounsaturated fat do not have this effect. As a consequence of this, the rate of removal of cholesterol of dietary origin from the body is slower in animals fed saturated as compared to monounsaturated or n-6 polyunsaturated fat. Thus, differential metabolism of chylomicron cholesterol clearly plays an important role in the hyperand hypo-cholesterolaemic effects of these dietary fats.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Comparison of the Uptake and Processing of Cholesterol from Chylomicrons of Different Fatty Acid Composition in Rats Fed High‐Fat and Low‐Fat Diets

Bravo

Flora

Cantàfora

et al. 1997

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…Polyunsaturated fatty acids have higher affinity than more saturated fatty acids for fatty acid binding protein (FABP; 46), and therefore are transported more rapidly within the mucosal cell. The larger chylomicrons produced after ingestion of polyunsaturated fatty acids are more rapidly cleared (47-49) because of different susceptibility to lipolytic enzymes (48,50). We observed greater chylomicronemia after ingestion of sunflower oil, which is not consistent with accelerated catabolism of polyunsaturated fatty acid-rich chylomicrons but is consistent with poor digestibility of beef tallow (14).…”

Section: Discussionmentioning

confidence: 63%

Intestinal absorption of β-carotene ingested with a meal rich in sunflower oil or beef tallow: postprandial appearance in triacylglycerol-rich lipoproteins in women

Jandacek

White

2000

The American Journal of Clinical Nutrition

View full text Add to dashboard Cite

Background: Evidence indicates that different types of fat have different effects on the postprandial plasma triacylglycerol response. Therefore, the type of fat may influence the appearance of ␤-carotene in postprandial triacylglycerol-rich lipoproteins, which is used as an indicator of intestinal ␤-carotene absorption. Objective: We compared in female subjects the appearance of ␤-carotene in plasma triacylglycerol-rich lipoproteins after ␤-carotene was ingested with a meal containing sunflower oil or beef tallow. Design: Women (n = 11) each ingested 2 different vitamin A-free, fat-rich meals that were supplemented with ␤-carotene (47 mol) and contained equivalent amounts (60 g) of sunflower oil or beef tallow. Blood samples were collected hourly from 0 to 10 h; additional samples were collected at selected intervals until 528 h. In a subgroup of the women (n = 7), plasma chylomicrons and 3 subfractions of VLDLs were separated by cumulative rate ultracentrifugation. Results: The appearance of ␤-carotene in chylomicrons and in each VLDL subfraction was lower after ingestion with the meal containing sunflower oil than after ingestion with the meal containing beef tallow (P < 0.03). In chylomicrons, the area under the concentration-versus-time curve (AUC) for ␤-carotene was 38.1 ± 13.6% lower (P < 0.03); in contrast, the AUC for triacylglycerol was higher (P < 0.05) after the sunflower-oil-rich meal than after the beef-tallow-rich meal. Conclusions: Ingestion of ␤-carotene with a meal rich in sunflower oil as compared with a meal rich in beef tallow results in lower appearance of ␤-carotene and greater appearance of triacylglycerol in triacylglycerol-rich lipoproteins.

show abstract

“…Hepatic lipase has been shown to promote the uptake of chylomicron remnants by livers from human subjects (Brekenridge et al 1982) and rats (Daggy & Bensadoun, 1986). However, as both the activity (Coiffer et al 1987) and the hepatic expression of mRNA (Bravo et al 1997) for this enzyme have been found to be increased to a greater extent in rats fed on saturated as compared with n-6 polyunsaturated fats, it seems unlikely to be involved in the changes in remnant uptake observed in the present work.…”

Section: Discussionmentioning

confidence: 99%

“…In the longer term the type of dietary fat consumed is likely to influence the fatty acid composition of the liver membranes (Kritchevsky et al 1988;Hostmark et al 1989) and the activity of hepatic lipase (EC 3.1.1.3) (Coiffer et al 1987;Bravo et al 1997). This may have additional effects on the rates of removal and metabolism of chylomicron remnants by the liver.…”

mentioning

confidence: 99%

Comparison of short- and long-term effects of different dietary fats on the hepatic uptake and metabolism of chylomicron remnants in rats

et al. 1998

View full text Add to dashboard Cite

The uptake and metabolism of [ ''C]oleate-labelled chylomicron remnants derived from olive oil, maize oil, palm oil, fish oil or butter fat was investigated using perfused livers from rats fed on the corresponding fat-supplemented diet (providing 40 % of the dietary energy) or a low-fat diet for 21 d. The percentage of added ['4C]oleate-labelled remnant removed from the perfusate was similar for livers from rats fed on the fat-supplemented diets irrespective of the type of fat fed, whereas livers from rats fed on the low-fat diet removed more labelled fish oil and butter fat remnants than olive, maize or palm oil remnants. Following hepatic uptake in the fatsupplemented groups, the oxidation of ['4C]oleate-labelled remnant lipid from maize oil, fish oil, and butter fat remnants was greater than that of the lipids from olive and palm oil remnants, although only the oxidation of lipids from maize and palm oil remnants was increased by prior fat-supplementation of the diet. In addition, the livers from rats fed on the fish-oil-supplemented diet incorporated more ['4C]oleate-labelled remnant lipid into phospholipid compared with the livers from rats fed on the other fat-supplemented diets or the low-fat diets. These investigations show that both prior fat feeding and the composition of the fat fed, as well as the fatty acid composition of the chylomicron remnant particles themselves, influence the uptake and metabolism of chylomicron remnants by the liver. Perfused rat liver: Dietary fat: ChylomicronsChylomicron remnants are formed by the action of lipoprotein lipase (EC 3.1.1.34) on triacylglycerol-rich chylomicrons, and are responsible for the delivery of dietary cholesterol and some triacylglycerol to the liver (Redgrave, 1970). Several studies have shown that the type of dietary fat from which chylomicrons and chylomicron remnants are derived influences their removal by the liver. Livers from dogs and rats are reported to remove [3H]-or [ 14C]fatty acid derived from labelled chylomicrons more rapidly when they are derived from cream as compared with maize oil (Nestel & Scow, 1964;Floren & Nilsson, 1977). Also, in human subjects, retinyl palmitate-labelled chylomicrons and chylomicron remnants derived from soyabean oil or cream are removed more rapidly from the circulation than those derived from olive oil (De Bruin et al. 1993). In this laboratory we have shown that ['4C]oleate-labelled fish oil and butter fat chylomicron remnants (labelled predominantly in triacylglycerol) are taken up by the perfused rat liver more rapidly than those derived from olive, maize or palm oils (Lambert et al. 1995). Taken together these studies show that chylomicron remnants derived from milk fat (cream or butter fat), soyabean oil or fish oil tend to be taken up more rapidly by the liver than those derived from olive, maize or palm oils. As fat-supplemented diets were not used in these studies, the differential uptake of the remnants can be attributed to the fatty acid composition of the particles themselves, which is largely dete...

show abstract

Effects of dietary saturated and polyunsaturated fat on lipoprotein lipase and hepatic triglyceride lipase activity

Cited by 14 publications

References 29 publications

Comparison of the Uptake and Processing of Cholesterol from Chylomicrons of Different Fatty Acid Composition in Rats Fed High‐Fat and Low‐Fat Diets

Comparison of the Uptake and Processing of Cholesterol from Chylomicrons of Different Fatty Acid Composition in Rats Fed High‐Fat and Low‐Fat Diets

Intestinal absorption of β-carotene ingested with a meal rich in sunflower oil or beef tallow: postprandial appearance in triacylglycerol-rich lipoproteins in women

Comparison of short- and long-term effects of different dietary fats on the hepatic uptake and metabolism of chylomicron remnants in rats

Contact Info

Product

Resources

About