Effects of dietary high fat on prostate intraepithelial neoplasia in TRAMP mice

Park, Sunghoon; Chang, Seo Il; Baek, Min Won; Kim, Dong-Jae; Na, Yirang; Seok, Seung Hyeok; Lee, Byoung-Hee; Kim, Kyung-Sul; Park, Jae‐Hak

doi:10.5625/lar.2013.29.1.39

Cited by 11 publications

(7 citation statements)

References 38 publications

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“…We concluded that HFD could promote TRAMP mice PCa development and progression. Similar findings were reported by Llaverias et al [ 12 ], Bonorden et al [ 13 ], Park et al [ 14 ], and Chang et al [ 15 ]. Although emerging evidence revealed that HFD was closely related with PCa development and progression, no clear mechanism was uncovered yet.…”

Section: Introductionsupporting

confidence: 91%

Proinflammatory Cytokines in Prostate Cancer Development and Progression Promoted by High-Fat Diet

Bai

et al. 2015

BioMed Research International

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Background. We aimed to examine whether proinflammatory cytokines participated in prostate cancer (PCa) development and progression promoted by high-fat diet (HFD). Methods. TRAMP (transgenic adenocarcinoma mouse prostate) mice were randomly divided into two groups: normal diet group and HFD group. Mortality rate and tumor formation rate were examined. TRAMP mice were sacrificed and sampled on the 20th, 24th, and 28th week, respectively. Levels of proinflammatory cytokines, including IL-1α, IL-1β, IL-6, and TNF-α, were tested by FlowCytomix. Prostate tissue of TRAMP mice was used for histology study. Results. A total of 13 deaths of TRAMP mice were observed, among which 3 (8.33%) were from the normal diet group and 10 (27.78%) from the HFD group. The mortality rate of TRAMP mice from HFD group was significantly higher than that of normal diet group (P = 0.032). Tumor formation rate at 20th week of age of HFD group was significantly higher than that of normal diet group (P = 0.045). Proinflammatory cytokines levels, including IL-1α, IL-1β, IL-6, and TNF-α, were significantly higher in HFD TRAMP mice. Conclusions. HFD could promote TRAMP mouse PCa development and progression with elevated proinflammatory cytokines levels. Proinflammatory cytokines could contribute to PCa development and progression promoted by HFD.

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Section: Introductionsupporting

confidence: 91%

Proinflammatory Cytokines in Prostate Cancer Development and Progression Promoted by High-Fat Diet

Bai

et al. 2015

BioMed Research International

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“…TRAMP mice fed a Western diet (containing 21.2% fat and 0.2% cholesterol) for 20 weeks also show increased histopathological scores compared to those eating a chow diet (containing 4.5% fat and 0.002% cholesterol) . We previously reported that TRAMP mice fed a high‐fat (45% Kcal fat) diet produce consistent results compared to TRAMP mice fed a control (16% Kcal fat) diet when mice were fed each diet for 10 weeks . In the present study, the histopathological score increased with age and high‐fat diet consumption.…”

Section: Discussionsupporting

confidence: 74%

High animal fat intake enhances prostate cancer progression and reduces glutathione peroxidase 3 expression in early stages of TRAMP mice

et al. 2014

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“…for example, the study by Wang et al showed that a low-fat diet decreased the tumor growth of xenografted lncaP cells (23). another study demonstrated that a high-fat diet induced a high level of serum leptin and high argyrophilic nucleolar organizer region (agnor) counts in a transgenic adenocarcinoma of mouse prostate (traMP) model (24). these studies suggested that chronic hyperleptinemia might be related to Pca progression.…”

Section: Discussionmentioning

confidence: 94%

Long-term exposure to leptin enhances the growth of prostate cancer cells

Noda

Kikugawa

Tanji

et al. 2015

International Journal of Oncology

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Obesity correlates with an increased risk of developing prostate cancer (PCa) and leptin plays an important role in PCa progression. Since leptin is produced by adipocytes, the serum leptin level is higher in obese than in non-obese individuals. However, the effects of leptin remain controversial and unclear. The aim of the present study was to investigate the effect of leptin on PCa cell aggressiveness. Three human PCa cell lines (LNCaP, DU145 and PC-3) were treated with recombinant leptin for 28 days. Cell proliferation, migration, and invasion were estimated using the WST assay, a wound-healing assay, and a BD Matrigel invasion assay, respectively. The mechanism underlying the proliferative effect of leptin was investigated by cell transfections with small interfering RNA (siRNA) against the leptin receptor (ObR) or forkhead box O1 (FOXO1), and by immunocytochemistry. Long-term exposure of PCa cells to leptin enhanced their proliferation, migration and invasion. Leptin increased ObR expression and enhanced Akt phosphorylation constitutively. Leptin also increased the phosphorylation of FOXO1 via PI3K signaling and FOXO1 gene silencing enhanced PCa cell proliferation. Leptin induced the translocation of FOXO1 from the nucleus to the cytoplasm. Furthermore, the PI3K inhibitor, LY294002 suppressed this translocation. These results suggested that leptin regulated the subcellular localization of FOXO1 and induced Akt phosphorylation. Additionally, we revealed that leptin increased the expression of cyclin D1 and decreased the expression of p21 protein. In conclusion, long-term exposure to leptin increased the cell proliferation, migration, and invasion of PCa cells through inactivation of FOXO1. This inactivation resulted from exclusion of FOXO1 from the nucleus and its restriction to the cytoplasm through PI3K/Akt signaling. Our findings contribute to an understanding of the association between obesity and PCa aggressiveness.

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Effects of dietary high fat on prostate intraepithelial neoplasia in TRAMP mice

Cited by 11 publications

References 38 publications

Proinflammatory Cytokines in Prostate Cancer Development and Progression Promoted by High-Fat Diet

Proinflammatory Cytokines in Prostate Cancer Development and Progression Promoted by High-Fat Diet

High animal fat intake enhances prostate cancer progression and reduces glutathione peroxidase 3 expression in early stages of TRAMP mice

Long-term exposure to leptin enhances the growth of prostate cancer cells

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