Effects of dietary fats on the fluidity of human high-density lipoprotein: Influence of the overall composition and phospholipid fatty acids

Solà, Rosa; Baudet, M.F.; Motta, Claude; Maillé, M.; Boisnier, C; Jacotot, B.

doi:10.1016/0005-2760(90)90108-a

Cited by 67 publications

(53 citation statements)

References 33 publications

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“…However, the most abundant membrane lipid, PC, was unchanged, indicating that LDL would have an altered membrane composition. Because CER, SM and FC have all been shown to positively affect a closer lateral packing in LDL [20,21], the altered lipid composition of LDL isolated from dyslipidaemic type 2 diabetic individuals could be associated with higher membrane fluidity and higher freedom in lateral moving of lipoprotein-associated proteins. Although the metabolic consequences of the reduced lipoprotein SM levels remain to be elucidated, it has been proposed that a decreased SM:PC ratio could render Fig.…”

Section: Discussionmentioning

confidence: 99%

Clinical dyslipidaemia is associated with changes in the lipid composition and inflammatory properties of apolipoprotein-B-containing lipoproteins from women with type 2 diabetes

et al. 2012

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Aims/hypothesis The aim of this study was to use lipidomics to determine if the lipid composition of apolipoprotein-Bcontaining lipoproteins is modified by dyslipidaemia in type 2 diabetes and if any of the identified changes potentially have biological relevance in the pathophysiology of type 2 diabetes. Methods VLDL and LDL from normolipidaemic and dyslipidaemic type 2 diabetic women and controls were isolated and quantified with HPLC and mass spectrometry. A detailed molecular characterisation of VLDL triacylglycerols (TAG) was also performed using the novel ozone-induced dissociation method, which allowed us to distinguish vaccenic acid (C18:1 n-7) from oleic acid (C18:1 n-9) in specific TAG species. Results Lipid class composition was very similar in VLDL and LDL from normolipidaemic type 2 diabetic and control participants. By contrast, dyslipidaemia was associated with significant changes in both lipid classes (e.g. increased diacylglycerols) and lipid species (e.g. increased C16:1 and C20:3 in phosphatidylcholine and cholesteryl ester and increased C16:0 [palmitic acid] and vaccenic acid in TAG). Levels of palmitic acid in VLDL and LDL TAG correlated with insulin resistance, and VLDL TAG enriched in palmitic acid promoted increased secretion of proinflammatory mediators from human smooth muscle cells. Conclusions We showed that dyslipidaemia is associated with major changes in both lipid class and lipid species composition in VLDL and LDL from women with type 2 diabetes. In addition, we identified specific molecular lipid species that both correlate with clinical variables and are proinflammatory. Our study thus shows the potential of advanced lipidomic methods to further understand the pathophysiology of type 2 diabetes.

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Section: Discussionmentioning

confidence: 99%

Clinical dyslipidaemia is associated with changes in the lipid composition and inflammatory properties of apolipoprotein-B-containing lipoproteins from women with type 2 diabetes

et al. 2012

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“…It was also demonstrated that alteration of the diet fatty acid composition (particularly phospholipid fatty acids) of normolipidemic subjects modified the metabolic activity of lipoproteins HDL and LDL [7,8]. The effect of fatty acid composition on lipoprotein fluidity could explain these changes in metabolic activities [9]. Few studies have been published on the fatty acid composition of lipoproteins in hyperlipidemic, particularly hypertriglyceridemic patients [10].…”

Section: Discussionmentioning

confidence: 99%

“…They were also increased slightly in the LDL. It has been suggested that polyunsaturated fatty acids may influence the structure of LDL [19] and HDL [9] by changing the fluidity of their constituent lipids. Thus, the increased proportion of polyunsaturated fatty acids due to linoleic acid in the HDL of HTG2 may increase the fluidity of this fraction in this group of patients.…”

Section: Discussionmentioning

confidence: 99%

Compositions of Serum Lipids, Lipoproteins, and Lipoprotein Fatty Acids in Type IV Primary Hypertriglyceridemia.

Agheli

Jacotot

Ayrault-Jarrier

et al. 1992

J. Clin. Biochem. Nutr.

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SummaryThe overall composition of serum lipoproteins and the fatty acid composition of cholesterol esters and phospholipids in the VLDL, IDL, LDL, and HDL were studied in eleven patients with familial type IV primary hypertriglyceridemia. The patients were divided into two groups: HTG 1 (serum triglycerides below 350 mg/dl: 200-294, n = 5) and HTG2 (serum triglycerides above 350 mg/dl: 368-820, n = 6). The data were compared with those from 9 normolipidemic subjects.The proportion of the constituents of VLDL was normal in the HTG 1 group, but cholesterol esters were increased and triglycerides decreased in this fraction in the HTG2 one. The increase in triglycerides and decrease in cholesterol esters in LDL and HDL modified the CE/TG and TG/total lipoprotein ratios in both groups of patients. The core lipid/surface ratio was increased in HDL in both groups, but not in the other three fractions.The saturated fatty acids of cholesterol esters were low (p<0.01) in the VLDL of the HTG2 group. The w6 fatty acids of cholesterol esters and phospholipids were slightly increased (p<0.05) in the VLDL in the same group. However, there was a decrease in the c6 fatty acids in cholesterol esters (p<0.05) and in the phospholipids (p<0.01) in the IDL of the HTG 1 group. There was no significant difference in the LDL fatty acid composition between the hypertriglyceridemic patients and the controls. The phospholipids of the HDL in the HTG2 group contained less (p.(0.05) saturated fatty acids and more (p<0.05) polyunsaturated fatty acids than did those in the HTG 1 group.These observations suggest that the fatty acid composition of lipoproteins in hypertriglyceridemic subjects is not markedly altered and is

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“…Moreover, several lipids that are common constituents of HDL are known to significantly affect the fluidity of lipid surfaces (e.g., PC and SPM) (15). Indeed, the phospholipid fatty acyl composition of lipoproteins is known to have subtle but measurable effects on the fluidity of the lipoprotein phospholipidic layer (15,16). These changes may affect the ability of HDL particles to accommodate FC molecules that have desorbed from peripheral cells.…”

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confidence: 99%

Age-related impairment of HDL-mediated cholesterol efflux

Berrougui

Isabelle

Cloutier

et al. 2007

Journal of Lipid Research

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Our aim in this study was to investigate the effect of aging on the capacity of HDLs to promote reverse cholesterol transport. HDLs were isolated from plasma of young (Y-HDL) and elderly (E-HDL) subjects. HDL-mediated cholesterol efflux was studied using THP-1 and J774 macrophages. Our results show that E-HDLs present a lower capacity to promote cholesterol efflux than Y-HDLs (41.7 6 1.4% vs. 49.0 6 2.2%, respectively; P 5 0.013). Reduction in the HDL-mediated cholesterol efflux capacity with aging was more significant with HDL 3 than HDL 2 (Y-HDL 3 , 57.3 6 1% vs. E-HDL 3 , 50.9 6 2%; P 5 0.012). Moreover, our results show that ABCA1-mediated cholesterol efflux is the more affected pathway in terms of cholesterol-removing capacity. Interestingly, the composition and structure of HDL revealed a reduction in the phosphatidylcholine-sphingomyelin ratio (E-HDL, 32.7 6 2.7 vs. Y-HDL, 40.0 6 1.9; P 5 0.029) and in the phospholipidic layer membrane fluidity in E-HDL compared with Y-HDL as well as an alteration in the apolipoprotein A-I structure and charge. In conclusion, our results shown that E-HDLs present a reduced capacity to promote cholesterol efflux, principally through the ABCA1 pathway, and this may explain the increase of the incidence of cardiovascular diseases observed during aging.-Berrougui, H., M. Isabelle, M. Cloutier, G. Grenier, and A. Khalil. Age-related impairment of HDL-mediated cholesterol efflux. J. Lipid Res. 2007. 48: 328-336.

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Effects of dietary fats on the fluidity of human high-density lipoprotein: Influence of the overall composition and phospholipid fatty acids

Cited by 67 publications

References 33 publications

Clinical dyslipidaemia is associated with changes in the lipid composition and inflammatory properties of apolipoprotein-B-containing lipoproteins from women with type 2 diabetes

Clinical dyslipidaemia is associated with changes in the lipid composition and inflammatory properties of apolipoprotein-B-containing lipoproteins from women with type 2 diabetes

Compositions of Serum Lipids, Lipoproteins, and Lipoprotein Fatty Acids in Type IV Primary Hypertriglyceridemia.

Age-related impairment of HDL-mediated cholesterol efflux

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