Effects of dietary copper, citric acid, and microbial phytase on digesta pH and ileal and carcass microbiota of broiler chickens fed a low available phosphorus diet

Aydın, Ali; Pekel, A. Y.; Issa, Ghassan; Demirel, Gülcan; Patterson, Paul H.

doi:10.3382/japr.2009-00123

Cited by 33 publications

(21 citation statements)

References 33 publications

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“…However, the proportion of Ins(1,2,3,4,6)P 5 in ΣInsP 5 was significantly lower, and concentrations of Ins(1,2,3,4)P 4 tended to be lower for the phytase treatments compared with the BD treatment in the duodenum/jejunum and lower ileum. Supplementation of phytase tends to reduce lactic acid bacterial count and significantly reduce E. coli count in the ileal digesta of broilers ( 63 ) , and, as mentioned earlier, both bacterial groups were suspected to be involved in phytate degradation. Aydin et al ( 63 ) speculated that the decrease is related to a possible reduction in the quantity of substrate available to the intestinal microbiota.…”

Section: Discussionmentioning

confidence: 83%

“…Supplementation of phytase tends to reduce lactic acid bacterial count and significantly reduce E. coli count in the ileal digesta of broilers ( 63 ) , and, as mentioned earlier, both bacterial groups were suspected to be involved in phytate degradation. Aydin et al ( 63 ) speculated that the decrease is related to a possible reduction in the quantity of substrate available to the intestinal microbiota. If there was a reduction of lactic acid bacteria (for example, Bifidobacteria ) this might explain the decrease in 5-phytase activity for all phytase-containing treatments whereas a reduction of E. coli bacteria might explain the decrease in 6-phytase activity for PhyA.…”

Section: Discussionmentioning

confidence: 83%

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Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers

et al. 2015

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The objective was to characterise degradation of myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate) (InsP6) and formation of inositol phosphate (InsP) isomers in different segments of the broiler digestive tract. Influence of an Aspergillus niger (PhyA) and two Escherichia coli-derived (PhyE1 and PhyE2) phytases was also investigated. A total of 600 16-d-old broilers were allocated to forty floor pens (ten pens per treatment). Low-P (5·2 g/kg DM) maize–soyabean meal-based diets were fed without (basal diet; BD) or with a phytase added. On day 25, digesta from different digestive tract segments were pooled per segment on a pen-basis, freeze-dried and analysed for P, InsP isomers and the marker TiO2. InsP6 degradation until the lower ileum (74 %) in BD-fed birds showed a high potential of broilers and their gut microbiota to hydrolyse InsP6 in low-P diets. Different InsP patterns in different gut segments suggested the involvement of phosphatases of different origin. Supplemented phytases increased InsP6 hydrolysis in the crop (P < 0·01) but not in the lower ileum. Measurements in the crop and proventriculus/gizzard confirmed published in vitro degradation pathways of 3- and 6-phytases for the first time. In the intestinal segments, specifically formed InsP4–5 isomers of supplemented phytases were still present, indicating further activity of these enzymes. Myo-inositol tetrakisphosphate (InsP4) accumulation differed between PhyE1 and PhyE2 compared with PhyA in the anterior segments of the gut (P < 0·01). Thus, the hydrolytic cleavage of the first phosphate group is not the only limiting step in phytate degradation in broilers.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 83%

Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers

et al. 2015

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“…was more frequent in the ileum and caecum of hens fed a diet enriched with F than of those fed a diet without F. This result was in contrast to that of Lu et al (2009) in which no enzyme treatment (F or xylanase) affected the lactobacillus or the anaerobic content in the ileal digesta of broilers. However, Aydin et al (2010) found that in the ileal digesta the viable counts of anaerobic bacteria, Escherichia coli, and coliform bacteria were significantly reduced among the broilers fed diets with F. The possible changes in the gastrointestinal tract caused by F may not only modify the pH of the ileum, but also may affect the digestibility of nutrients. Moreover, the addition of dietary F might improve performance, energy, and amino acid availability (Cowieson et al 2006).…”

Section: Discussionmentioning

confidence: 94%

Effects of a low-phosphorus diet and exogenous phytase on performance, egg quality, and bacterial colonisation and digestibility of minerals in the digestive tract of laying hens

Englmaierová¹,

Skřivan²,

Skřivanová³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:The objective of the experiment was to determine the effects of different levels of phytase (0, 150, 250, and 350 phytase units (FTU)/kg; F) produced by Aspergillus niger in the diets of hens fed decreased contents of non-phytate phosphorus (1.8 and 2.1 g/kg; NPP) on the hen performance, egg quality, digestibility of calcium and phosphorus in the ileum, and representation of bacterial species in the ileum and caecum. The diet with 2.1 g/kg of NPP and 350 FTU/kg significantly decreased egg weight (P < 0.001) and egg mass production (P < 0.001). However, this treatment had the highest values for shell percentage (P = 0.002), shell thickness (P = 0.006), and shell index (P = 0.003). The supplementation with F at 350 FTU/kg to the diet with 1.8 g/kg of NPP increased the shell quality to a level that was comparable with the eggs from the hens fed the diet with only 2.1 g/kg of NPP. With the addition of F (350 FTU/kg) to the mixed feed with 1.8 g/kg of NPP, the digestibility of calcium and phosphorus in the ileum increased by 6.1% and 7.4%, respectively, although the increases were not significant. Additionally, the frequency of Lactobacillus spp. was higher in the ileum and caecum of hens fed the diet enriched with F than in those fed a diet without F addition. The dietary manipulations with NPP and F improved some performance and shell quality characteristics, and the addition of 3-phytase at a level of 350 FTU per kg to the low-P diet increased the digestibility of minerals and changed the microflora of the digestive tract.

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“…In addition, supplementation with 750 FTU of F significantly reduced the log 10 counts for total anaerobic bacteria, Escherichia coli, and coliform bacteria in the ileal digesta of chicks (0.28, 0.22, and 0.59 log 10 , respectively). Phytase supplementation might effectively decrease ileal pathogenic bacterial populations and beneficially affect gut health (Aydin et al 2010). As evident from the aforementioned facts, the effect of F on intestinal microbial composition undoubtedly merits further investigation.…”

Section: Discussionmentioning

confidence: 99%

Limestone particle size and Aspergillus niger phytase in the diet of older hens

Englmaierová

Skřivan

Skřivanová

et al. 2017

Italian Journal of Animal Science

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The aim of this experiment was to evaluate the influence of limestone particle size and phytase (F) on performance, physical egg parameters, mineral digestibility and microbial colonisation in the digestive tract in older hens aged 60-71 weeks. One hundred and sixty Lohmann Brown hens were housed in enriched cages. The 2 Â 2 factorial experiment included two levels of 3-phytase NATUPHOSV R (0 and 350 FTU/kg), two sizes of limestone grain (coarse limestone (CL) and a mix of CL and fine limestone (FL) (1:1)) in mixed feed. All of the diets contained 4.4 g/kg of total phosphorus and 34 g/kg of calcium. Coarse limestone with F addition significantly increased hen-day egg production (p ¼ .018). The phytase supplement at 350 FTU/kg significantly increased shell quality characteristics. Haugh units (p ¼ .039) and albumen index (p ¼ .008) was higher when using the limestone mix, the same applies to yolk index (p ¼ .013). The phytase addition to the mixed feed non-significantly increased calcium and phosphorus digestibility in the ileum by 3.1 and 3.9%, respectively. Intestinal microbiota from each sample did not cluster according to the treatment; however, bacteria belonging to the genera Turicibacter and Lactobacillus occurred with higher frequency in the ileum of hens that were fed mixed feed enriched with phytase. In conclusion, limestone particle size (1 to 2 mm) with F addition at 350 FTU/kg in a wheat-maize-soybean meal-based diet increased egg production while maintaining egg content and eggshell quality in older hens. Ileal microbiota was influenced by F supplementation in the diet. ARTICLE HISTORY

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Effects of dietary copper, citric acid, and microbial phytase on digesta pH and ileal and carcass microbiota of broiler chickens fed a low available phosphorus diet

Cited by 33 publications

References 33 publications

Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers

Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers

Effects of a low-phosphorus diet and exogenous phytase on performance, egg quality, and bacterial colonisation and digestibility of minerals in the digestive tract of laying hens

Limestone particle size and Aspergillus niger phytase in the diet of older hens

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