Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers

Zeller, Ellen; Schollenberger, Margit; Kühn, Imke; Rodehutscord, M.

doi:10.1017/jns.2014.62

Cited by 114 publications

(125 citation statements)

References 57 publications

(70 reference statements)

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“…Concentrations of Ti were determined by a modification of the sulphuric acid and nitric acid wet digestion method (Boguhn et al 2009) as described by Zeller et al (2015b). This was done on a pen-bypen basis to ensure the same time schedule for all pens.…”

Section: Sampling and Analytical Methodsmentioning

confidence: 99%

Dietary effects on inositol phosphate breakdown in the crop of broilers

Zeller

Schollenberger

Kühn

et al. 2015

Archives of Animal Nutrition

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The effect of diets differing in enzyme supplements, mineral phosphorus (P) and microwave wheat treatment on phytate hydrolysis and lower inositol phosphate isomers (InsPs) appearance in broiler crops was studied. The broilers (16- and 15-day-old) were assigned to 48 pens of 15 or 20 birds each (n = 8 pens per treatment) in Experiments 1 and 2, respectively. In Experiment 1, birds received a low-P wheat-soybean meal diet where the wheat was either microwave treated or not. These diets were offered without further supplementation or with added phytase (500 FTU/kg diet), alone or in combination with a xylanase (16,000 BXU/kg diet). In Experiment 2, two maize-soybean meal-based diets were fed, without or with monocalcium phosphate supplementation. Furthermore, these diets were offered without further supplementation or with phytase at 500 or 12,500 FTU/kg diet. On day 23 or 24 (Experiments 1 and 2, respectively), crop digesta were pooled per pen, freeze-dried and analysed for InsPs and the marker TiO2. Microwaving reduced the intrinsic phytase activity and InsP6 hydrolysis, but increased the concentration of Ins(1,2,3,4,5)P5 and Ins(1,2,4,5,6)P5 in the digesta of crop (Experiment 1). Microwave treatment significantly interacted with enzyme supplementation for Ins(1,2,5,6)P4 concentration, indicating a synergistic effect of intrinsic and supplied phytase in the crop. Xylanase tended to support phytase hydrolysis in diets with microwave-treated wheat. Phytase addition increased InsP6 hydrolysis up to 79% (Experiment 2). Thus, wheat phytase activity can cause high InsP6 hydrolysis in the crop. Treatment differences in lower InsPs indicated that hydrolysis of the first InsP6 phosphate group is not the only step in the degradation cascade in the crop of broilers that is influenced by dietary factors.

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Section: Sampling and Analytical Methodsmentioning

confidence: 99%

Dietary effects on inositol phosphate breakdown in the crop of broilers

Zeller

Schollenberger

Kühn

et al. 2015

Archives of Animal Nutrition

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“…The acids and ethanol are central metabolic intermediates and excellent substrates for the host and, provided that they are readily taken up from the digestive tract, cellular dehydrogenases convert them into intermediates that enter the citric acid cycle and subsequent ATP-producing electron In a crop with lower pH as a result of fermentation, conditions are optimised for some enzymes, both endogenous and exogenous (Zeller et al, 2016). In particular, it is well established that phytate hydrolysis in the crop can be extensive (Lan et al, 2010;Onyango et al, 2005;Svihus et al, 2010;Zeller et al, 2015;. Increased crop hydrolysis does not necessarily lead to differences in terminal ileum phytate disappearance (Zeller et al, 2015).…”

Section: Potential Effects On Bird Performancementioning

confidence: 99%

“…In particular, it is well established that phytate hydrolysis in the crop can be extensive (Lan et al, 2010;Onyango et al, 2005;Svihus et al, 2010;Zeller et al, 2015;. Increased crop hydrolysis does not necessarily lead to differences in terminal ileum phytate disappearance (Zeller et al, 2015). However, reducing phytate's ability to bind minerals and protein at this early stage of the digestive process should reduce its negative effects on nutrient digestibility (Yu et al, 2012).…”

Section: Potential Effects On Bird Performancementioning

confidence: 99%

The role of the crop in poultry production

Classen

Apajalahti

Svihus

et al. 2016

World's Poultry Science Journal

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The importance of the crop is often underestimated in poultry production. In addition to storing ingested feed, it also can impact nutrient digestion by digesta softening and the initial activity of feed (endogenous and exogenous) and microbial enzymes. The crop represents the first major defence against poultry pathogens and zoonotic organisms with well established adaptive and innate immune function, and a lactobacilli dominated microbiota capable of reducing the passage of these organisms further along the digestive tract. However, the potential to improve bird productivity and health, as well as affect meat and egg safety, are influenced by the nature of the diet, and in particular feed entry and extended presence in the crop. This is required to promote lactobacilli fermentation, the production of lactic acid and other volatile fatty acids, and the lowering of crop pH. Management practices such as meal feeding and the use of lighting programs with extended dark periods encourage crop utilisation. Further, the use of feed additives such as prebiotics and probiotics may enhance crop function, which in turn contributes to well-being of the entire digestive tract. A healthy and functional crop, along with other segments of digestive tract, has increased importance in an era of reduced antibiotic use in poultry feeds.

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“…Al reducir los niveles de fósforo inorgánicos en la dieta disminuye el porcentaje de cenizas en tibias, esto debido a que el 80 % del fósforo total del organismo es depositado en tejido óseo (22) . Los resultados mostraron que la adición de las fitasas microbianas procedentes de Citrobacter braakii o Escherichia coli mejoraron de manera similar la biodisponibilidad del fósforo proveniente del fósforo fítico de la pasta de soya y el sorgo; resultados similares reportan que el empleo de 500 FTU/kg fue eficaz en mejorar el crecimiento, cenizas en tibias y retención de fósforo en pollos de engorda, con la liberación de 0.08 % de fósforo disponible (23) .…”

Section: Discussionunclassified

Eficacia de dos fitasas bacterianas en la liberación de fósforo en dietas para pollos de engorda en crecimiento

Espinoza

Cuevas

González

2017

Rev. Mex. Cienc. Pecu.

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RESUMENLa eficacia de dos fitasas (Citrobacter brakii y E. Coli) utilizadas en diferentes dosis (500 y 1,000 FTU) se evaluaron en una dieta deficiente en fósforo (0.15% P disponible) formulada a base de sorgo-soya. Para la estimación de la eficacia en la liberación de P de las fitasas evaluadas se utilizó como referencia fósforo inorgánico suplementado a través de fosfato monodicálcico (FMD), para alcanzar una concentración final de P disponible de 0.23, 0.31 y 0.39 %. Para lo anterior la dieta basal deficiente en P se suplementó como sigue: 1) sin fitasas, sin FMD; 2) 0.08% FMD; 3) 0.16% FMD; 4) 0.24% PMD; 5) citrobacter-500 FTU; 6) coli-500 FTU; 7) citrobacter-1,000 FTU y 8) coli-1,000 FTU. Los tratamientos se aplicaron a 216 pollos de la estirpe Ross (27 pollos/tratamiento) de los 7 a 21 días de edad. La ganancia de peso, eficiencia alimenticia y concentración de ceniza y P en tibias fue mayor en los pollos que recibieron 0.39% PMD y los 1,000 FTU de ambas fitasas, no existiendo diferencias en estos niveles entre los tratamientos FMD y fitasas. La cantidad de fósforo liberado no se afectó (P >0.05) por el tipo de fitasa (promedio 0.142 %), pero sí por el nivel de fitasa utilizado (0.102 vs 0.182 % para 500 y 1,000 FTU respectivamente). Los resultados indican que la suplementación con 1,000 FTU/kg de ambas fitasas en dietas deficientes en P resulta en ganancias y eficiencias alimenticias similares cuando las dietas se suplementan hasta 0.24 % de FMD. Ambas fitasas mejoraron en 78.4 % el aprovechamiento del fósforo fítico en dietas sorgo-soya para pollos en crecimiento. PALABRAS CLAVE: Fitasas, Citrobacter braakii, Escherichia coli, Fósforo en tibias, Cenizas en tibias, Pollos de engorda. ABSTRACTThe efficacy of two phytases (Citrobacter brakii and Escherichia coli) used in different doses (500 and 1,000 FTU) were evaluated in a diet deficient in phosphorus (0.15% available P) formulated with sorghum-soybean meal. To estimate the efficacy of the release of P evaluated phytases, inorganic phosphorus supplemented by mono-phosphate (MDP) to reach a final concentration of available P of 0.23, 0.31 and 0.39 % was used as reference. The basal diet deficient in P was supplemented as follows: 1) Without phytases, without MDP; 2) 0.08% MDP; 3) 0.16% MDP; 4) 0.24% MDP; 5) Citrobacter-500 FTU; 6) coli FTU-500; 7) citrobacter-1,000 FTU and 8) FTU coli-1,000. The treatments were applied to Ross 216 chickens (27 birds/treatment) 7 to 21 d old. Weight gain, feed efficiency and concentration of tibia ash and P was better in chickens receiving 0.39% PMD and 1,000 FTU of both phytase, with no differences in these levels between MDP and phytase treatments. The amount of phosphorus released was not affected (P >0.05) by type of phytase (average 0.142 %), with effect to level of phytase used (0.102 vs 0.182 % for 500 and 1,000 FTU respectively). The results indicate that supplementation 1,000 FTU / kg in both phytase in diets deficient in P results in weight gain and feed efficiency similar when the diets are supplemented with 0.24...

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Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers

Cited by 114 publications

References 57 publications

Dietary effects on inositol phosphate breakdown in the crop of broilers

Dietary effects on inositol phosphate breakdown in the crop of broilers

The role of the crop in poultry production

Eficacia de dos fitasas bacterianas en la liberación de fósforo en dietas para pollos de engorda en crecimiento

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