Effects of dietary choline supplementation on growth performance and hepatic lipid transport in blunt snout bream (Megalobrama amblycephala) fed high-fat diets

Cloning and characterization of microsomal triglyceride transfer protein gene and its potential connection with peroxisome proliferator-activated receptor (PPAR) in blunt snout bream ( Megalobrama amblycephala )

“…Altogether, this data may suggest that adequate dietary choline could promote lipid transport and oxidation, enhancing the immune capacity and improving organ function of blunt snout bream. The change of liver PPARα mRNA expression coincides with that of the MTTP that was determined in our previous study (Li et al, 2014). These findings provide support to sustain the idea that MTTP is a downstream target gene of PPARα (Ame'en et al, 2005;Stefano et al, 2007).…”

“…Feeding trial and sample collection have been detailed in our previous study (Li et al, 2014). Briefly, six treatment groups of blunt snout bream (average weight: 9.80 ± 0.35 g) were fed with six isonitrogenous (32% crude protein) semi-purified diets containing three lipid levels (5%, 8%, and 11%) and two choline supplementation levels (1200 and 1800 mg kg −1 ).…”

“…The extent of fat accumulation is determined by the balance between fatty acid uptake, oxidation, and synthesis, as well as triglyceride secretion through lipoproteins; thus, the variability of these pathways has a major impact on fat accumulation (Sander Kersten, 2002). Furthermore, our previous studies indicate that dietary choline is required for optimum growth and for the prevention of fat deposition (Jiang et al, 2013;Li et al, 2014Li et al, , 2015. In addition, we observe that extra choline supplements enhance VLDL synthesis and assembly through the up-regulating expressions of apolipoprotein B-100 and MTTP which subsequently promote hepatic lipid transport, and may partly account for the effects of choline on mitigating highfat induced abnormal physiological and metabolic activities in blunt snout bream (Li et al, 2014).…”

“…Furthermore, our previous studies indicate that dietary choline is required for optimum growth and for the prevention of fat deposition (Jiang et al, 2013;Li et al, 2014Li et al, , 2015. In addition, we observe that extra choline supplements enhance VLDL synthesis and assembly through the up-regulating expressions of apolipoprotein B-100 and MTTP which subsequently promote hepatic lipid transport, and may partly account for the effects of choline on mitigating highfat induced abnormal physiological and metabolic activities in blunt snout bream (Li et al, 2014). Regarding the crucial role of MTTP toward the lipid metabolism of fish species, the present study was conducted in order to clone the full-length cDNA of MTTP genes from the livers of juvenile blunt snout bream, and to analyze its distribution within several tissues.…”

Effects of dietary lipid levels on growth, fatty acid composition, antioxidant status and lipid metabolism in juvenileOnychostoma macrolepis

“…In the recent years, there has been a tendency of utilizing lipid‐rich diets in intensive aquaculture, because higher growth rates can be achieved by increasing dietary lipids above their minimum levels, probably as a result of protein sparing and improved feeding efficiency (Boujard et al, ; Lu et al, ). However, there will also be an upper limit that, if exceeded, will result in unwanted deposition of lipids in the tissues due to an imbalance of nutrient and energy supplies, and may negatively affect survival, growth, disease resistance, stress response and lipid metabolism (Chen et al, ; Company, Calduch‐Giner, Kaushik, & Perez‐Sanchez, ; Gaylord & Gatlin, ; Li et al, ; Lu et al, ; Regost et al, ; Wang et al, ).…”