Cloning and characterization of microsomal triglyceride transfer protein gene and its potential connection with peroxisome proliferator-activated receptor (PPAR) in blunt snout bream ( Megalobrama amblycephala )

Li, Junyi; Zhang, Ding-dong; Jiang, Guang‐Zhen; Li, Xiangfei; Zhang, Chunnuan; Zhou, Man; Liu, Wenbin; Xu, Weina

doi:10.1016/j.cbpb.2015.07.004

Cited by 4 publications

(5 citation statements)

References 70 publications

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“…2 ). Similar structural domains have also been found in MTP of H. sapiens , as well as in other MTP orthologs such as D. rerio , D. melanogaster , C. elegans ( 40 ), and M. amblycephala ( 44 ). The RMSD was found to be 2.52, 3.07, 3.07, and 10.2 Å for H. sapiens , D. melanogaster , X. tropicalis , and C. briggsae , respectively.…”

Section: Discussionsupporting

confidence: 70%

Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

Khan

Dalvin

Nilsen

et al. 2017

Journal of Lipid Research

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The salmon louse, Lepeophtheirus salmonis, is an endemic ectoparasite on salmonid fish that is challenging for the salmon farming industry and wild fish. Salmon lice produce high numbers of offspring, necessitating sequestration of large amounts of lipids into growing oocytes as a major energy source for larvae, most probably mediated by lipoproteins. The microsomal triglyceride transfer protein (MTP) is essential for the assembly of lipoproteins. Salmon lice have three L. salmonis MTP (LsMTP) transcript variants encoding two different protein isoforms, which are predicted to contain three β-sheets (N, C, and A) and a central helical domain, similar to MTPs from other species. In adult females, the LsMTPs are differently transcribed in the sub-cuticular tissues, the intestine, the ovary, and in the mature eggs. RNA interference-mediated knockdown of LsMTP in mature females gave offspring with significantly fewer neutral lipids in their yolk and only 10–30% survival. The present study suggests the importance of LsMTP in reproduction and lipid metabolism in adult female L. salmonis, a possible metabolic bottleneck that could be exploited for the development of new anti-parasitic treatment methods.

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Section: Discussionsupporting

confidence: 70%

Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

Khan

Dalvin

Nilsen

et al. 2017

Journal of Lipid Research

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“…Besides, a recent study claimed that, as a kind of physiological response to the increase of fat absorption, the mRNA expression of PPARα in liver enhanced with the increase of dietary fat levels (Rinella et al, 2008). However, in the study of fingerling blunt snout bream Megalobrama amblycephala, the mRNA expression of PPARα in liver significantly decreased by feeding high-fat (fat level of 11%, soybean oil as the fat source) diet (Li et al, 2015). The reason might be that the fat intake was far more than the demand of this species.…”

Section: Discussionmentioning

confidence: 76%

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Yang¹,

Wang²,

Liu³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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The full-length cDNA of proliferator-activated receptor alpha (PPARα) was abtained from the liver of redlip mullet, Liza haematocheila. The PPARα cDNA (GenBank no: KJ848472) was 2409 bp including a 1437 bp open reading frame, which encoded 478 amino acids with four signature domains, i.e., the hypervariable region in N-terminus, DNA-binding domain (DBD), flexible hinge domain and ligand-binding domain (LBD). The mRNA expression level of PPARα was detected in all tissues tested. Highest expression occurred in liver, followed by brain, stomach, skin, spleen and visceral fat, but the expression was weak in heart and muscle. Then, a 60-day feeding trial was conducted to study, the effects of dietary lipid levels (2.0%, 4.8%, 7.5%, 9.8%, 12.0% and 14.6 %) on the mRNA expression of PPARα in mullet. PPARα mRNA expression in liver increased significantly (P<0.05) with the increasing dietary lipid levels. These results indicated that PPARα was tissue-differential expressed gene and played a pivotal role in regulating the lipid metabolism mainly in liver. Results of this study will benefit the further researches on the relationships between PPARα gene and fat metabolism of redlip mullet.

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“…The liver of tiger puffer also had a high mttp expression level, about 100 times higher than that in the eye and skin, which had the lowest mttp expression level among the examined tissues. In another finfish species, blunt snout bream (Megalobrama amblycephala), the liver and intestine also had the highest mttp expression level, but the liver had a much higher expression level compared to the intestine [41]. In zebrafish, the anterior intestine had the highest mttp gene expression, followed by the posterior intestine and liver, which were much higher compared to other tissues [25].…”

Section: Resultsmentioning

confidence: 99%

“…In channel catfish (Ictalurus punctatus), a high-lipid diet decreased the expression of mttp in the liver [34]. However, in contrast, in blunt snout bream, an elevation of dietary lipid content from 5% to 11% significantly upregulated the mttp mRNA abundance in the muscle, intestine, and liver [41]. A hepatic transcriptome assay on tiger puffer has shown that in tiger puffer, the lipid and cholesterol metabolism were not sensitive to the change of lipid level in the diet [54].…”

Section: Regulation By Dietary Cholesterol Levelsmentioning

confidence: 99%

Tissue Distribution and Nutritional Regulation of Four Cholesterol Transport-Related Genes in Tiger Puffer (Takifugu rubripes)

Xiong,

Song,

Zhang

et al. 2024

Aquaculture Research

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This study characterized four cholesterol transport-related genes, namely, lcat, acat1, acat2, and mttp, in juvenile tiger puffer in terms of tissue distribution (eye, heart, brain, skin, liver, spleen, muscle, and intestine) and nutritional regulation. Three feeding trials were conducted: (i) using diets with different cholesterol levels (0.11%, 0.65%, 1.10%, 2.32%, and 4.59% of dry matter); (ii) using diets with different lipid levels (8.05%, 12.02%, and 16.36% of dry matter); and (iii) a 1-month starvation experiment with different sampling times (Days 1, 4, 9, 16, and 31). The lcat, acat1, and acat2 were the most abundantly expressed in the liver, while mttp was the most abundantly expressed in the intestine. The lcat had a medium expression level in the muscle and skin but the lowest expression level in the spleen and intestine. The acat1 and acat2 had similar tissue distribution patterns, except that acat2 had a lower expression level in the heart but a higher level in the intestine than acat1. The mttp had medium expression levels in the liver and spleen but had the lowest level in the eye and skin. Different dietary cholesterol levels did not significantly affect the expression of these cholesterol transport genes in the liver and intestine, except that higher cholesterol levels (1.10%–4.59%) significantly downregulated the acat2 expression in the liver. Dietary lipid levels also had a mild influence on the hepatic expression of these genes, except that the diet with 12.02% lipid resulted in significantly higher acat1 expression than the one with 16.36% lipid. The starvation time significantly affected the hepatic expression of these genes. Long-term starvation (16 and 31 days) generally upregulated the hepatic gene expression of lcat, acat1, and mttp but generally downregulated that of acat2. This study provided preliminary knowledge about the tissue distribution and nutritional regulation of cholesterol transport-related genes in marine teleost.

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Cloning and characterization of microsomal triglyceride transfer protein gene and its potential connection with peroxisome proliferator-activated receptor (PPAR) in blunt snout bream ( Megalobrama amblycephala )

Cited by 4 publications

References 70 publications

Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

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Tissue Distribution and Nutritional Regulation of Four Cholesterol Transport-Related Genes in Tiger Puffer (Takifugu rubripes)

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