Effects of dietary arachidonic acid on cortisol production and gene expression in stress response in Senegalese sole (Solea senegalensis) post-larvae

Martins, Dulce Alves; Rocha, Filipa; Castanheira, Filipa; Candeias‐Mendes, Ana; Pousão-Ferreira, Pedro; Bandarra, Narcisa M.; Coutinho, Joana; Morais, Sofía; Yúfera, Manuel; Conceição, Luı́s E.C.; Martı́nez-Rodrı́guez, Gonzalo

doi:10.1007/s10695-013-9778-6

Cited by 45 publications

(46 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, there were no significant differences in the ACTH-induced expression of the StAR gene immediately after the ACTH pulse, even when an increase in cortisol was detected in all experimental groups after stimulation with the trophic hormone. An increase in whole-body cortisol after stress without an increase in StAR gene expression has also been described in Senegalese sole post-larvae fed different ARA levels in the diet ( 20 ) . Those authors hypothesised that higher StAR transcription may not be necessary for cortisol production.…”

Section: Discussionmentioning

confidence: 80%

“…Moreover, the effect obtained by EPA incubation was faster in time (significantly different at 20 min after ACTH stimulation) than in the case of ARA, which showed a significant difference from the controls at 40 min after ACTH stimulation. Dietary ARA has been shown to affect whole-body cortisol and stress response in different marine fish larvae ( 17 , 18 , 20 , 52 , 53 ) including European sea bass ( 54 ) . However, since these studies were conducted in whole larvae, and ARA was supplied through either artemia or microdiets, the assignment of an ARA direct effect on whole-body cortisol of larvae is too premature ( 20 ) .…”

Section: Discussionmentioning

confidence: 99%

“…In particular, ALA has been shown to increase the in vitro release of cortisol from gilthead sea bream inter-renal cells, whereas LA produced the same effect but delayed in time ( 9 ) . As for the different essential fatty acids, the deficiency of n -3 highly unsaturated fatty acids has been shown to alter the post-stress plasma cortisol levels in gilthead sea bream ( 16 ) and ARA has been reported to affect whole-body cortisol levels in larval stages of this species ( 17 – 20 ) .…”

mentioning

confidence: 99%

“…In more recent studies, it has been shown that dietary fatty acids are able to modulate the expression of stress response-related genes in different marine fish species ( 20 , 22 ) , as it occurs in mammals ( 23 – 25 ) . The long-chain PUFA have been shown to down-regulate the expression of genes involved in the release of cortisol, such as StAR.…”

mentioning

confidence: 99%

“…In fish, the role of dietary lipids on StAR expression remains unclear. Only some effects of ARA in Senegalese sole whole post-larval StAR gene expression have been described ( 20 ) , without finding a clear correlation between dietary levels of ARA and the expression of this steroidogenic gene.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Modulation of adrenocorticotrophin hormone (ACTH)-induced expression of stress-related genes by PUFA in inter-renal cells from European sea bass (Dicentrarchus labrax)

et al. 2015

View full text Add to dashboard Cite

Dietary fatty acids have been shown to exert a clear effect on the stress response, modulating the release of cortisol. The role of fatty acids on the expression of steroidogenic genes has been described in mammals, but little is known in fish. The effect of different fatty acids on the release of cortisol and expression of stress-related genes of European sea bass (Dicentrarchus labrax) head kidney, induced by a pulse of adenocorticotrophin hormone (ACTH), was studied. Tissue was maintained in superfusion with 60 min of incubation with EPA, DHA, arachidonic acid (ARA), linoleic acid or α-linolenic acid (ALA) during 490 min. Cortisol was measured by RIA. The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real-time RT-PCR. There was an effect of the type of fatty acid on the ACTH-induced release of cortisol, values from ALA treatment being elevated within all of the experimental period. The expression of some steroidogenic genes, such as the steroidogenic acute regulatory protein (StAR) and c-fos, were affected by fatty acids, ALA increasing the expression of StAR after 1 h of ACTH stimulation whereas DHA, ARA and ALA increased the expression of c-fos after 20 min. ARA increased expression of the 11β-hydroxylase gene. Expression of heat shock protein 70 (HSP70) was increased in all the experimental treatments except for ARA. Results corroborate previous studies of the effect of different fatty acids on the release of cortisol in marine fish and demonstrate that those effects are mediated by alteration of the expression of steroidogenic genes.

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Modulation of adrenocorticotrophin hormone (ACTH)-induced expression of stress-related genes by PUFA in inter-renal cells from European sea bass (Dicentrarchus labrax)

et al. 2015

View full text Add to dashboard Cite

show abstract

Utilization of Live‐Food Enrichment with Polyunsaturated Fatty Acids (PUFA) for the Intensive Culture of Yellow Perch Larvae

Grayson

Dąbrowski

2022

N American J Aquac

View full text Add to dashboard Cite

Challenges associated with first feeding have impeded the intensive culture of larval Yellow Perch Perca flavescens. Live-food enrichment with polyunsaturated fatty acids (PUFAs) can increase the growth and survival of larval fish, but this method has not been tested with Yellow Perch. This study includes two experiments that were meant to evaluate (1) the relative proportion of docosahexaenoic acid (DHA; 22:6[n-3]) and arachidonic acid (ARA; 20:4[n-6]) in enrichment emulsions and (2) the provision of PUFA emulsions in ethyl ester (EE) or triacylglycerol (TG) form on Yellow Perch growth and survival. Fish were provided with live rotifers Brachionus plicatilis and brine shrimp Artemia nauplii for the first 10 d of exogenous feeding within a specialized recirculating system (phase I). Fish were then transferred to flow-through tanks and were fed Artemia nauplii for 3 d before gradually transitioning to a formulated starter diet (5-7 d; phase II). Fish size, growth, survival, swim bladder inflation rates, and lipid/fatty acid concentrations were evaluated after each phase. Overall, rotifers assimilated ARA in higher concentrations (23.3% of total fatty acids) than Artemia (0.6-0.7% of total fatty acids). Rotifers also tended to assimilate PUFAs better in EE form than in TG form (71.3% versus 66.5% Σn-3 + n-6 fatty acids). In the first experiment, fish from the ARA-and DHAenriched diet groups had greater swim bladder inflation rates (phase I) and growth rates (phase II) than the unenriched control group. In phase I of the second experiment, the EE enrichment group had a significantly faster growth rate than the TG enrichment group (specific growth rate: mean AE SD = 40.5 AE 0.9% and 36.6 AE 1.8%, respectively). Fatty acid composition of zooplankton was heavily influenced by enrichments, and fatty acid composition of larvae/ juveniles reflected that of their live prey. The results of this study suggest that PUFA enrichment of live feeds can accelerate Yellow Perch growth and reduce the time spent in the critical period of early development.

show abstract

Effects of Dietary Levels of PUFA Fed to Adult Yellow Perch on the Fatty Acid Composition of Eggs and Larvae Characteristics: New Research Directions

Dąbrowski

Rinchard

Czesny

et al. 2015

Biology and Culture of Percid Fishes

View full text Add to dashboard Cite

Effects of dietary arachidonic acid on cortisol production and gene expression in stress response in Senegalese sole (Solea senegalensis) post-larvae

Cited by 45 publications

References 74 publications

Modulation of adrenocorticotrophin hormone (ACTH)-induced expression of stress-related genes by PUFA in inter-renal cells from European sea bass (Dicentrarchus labrax)

Modulation of adrenocorticotrophin hormone (ACTH)-induced expression of stress-related genes by PUFA in inter-renal cells from European sea bass (Dicentrarchus labrax)

Utilization of Live‐Food Enrichment with Polyunsaturated Fatty Acids (PUFA) for the Intensive Culture of Yellow Perch Larvae

Effects of Dietary Levels of PUFA Fed to Adult Yellow Perch on the Fatty Acid Composition of Eggs and Larvae Characteristics: New Research Directions

Contact Info

Product

Resources

About