Utilization of Live‐Food Enrichment with Polyunsaturated Fatty Acids (PUFA) for the Intensive Culture of Yellow Perch Larvae

Abstract

Challenges associated with first feeding have impeded the intensive culture of larval Yellow Perch Perca flavescens. Live-food enrichment with polyunsaturated fatty acids (PUFAs) can increase the growth and survival of larval fish, but this method has not been tested with Yellow Perch. This study includes two experiments that were meant to evaluate (1) the relative proportion of docosahexaenoic acid (DHA; 22:6[n-3]) and arachidonic acid (ARA; 20:4[n-6]) in enrichment emulsions and (2) the provision of PUFA emulsions in ethyl ester (EE) or triacylglycerol (TG) form on Yellow Perch growth and survival. Fish were provided with live rotifers Brachionus plicatilis and brine shrimp Artemia nauplii for the first 10 d of exogenous feeding within a specialized recirculating system (phase I). Fish were then transferred to flow-through tanks and were fed Artemia nauplii for 3 d before gradually transitioning to a formulated starter diet (5-7 d; phase II). Fish size, growth, survival, swim bladder inflation rates, and lipid/fatty acid concentrations were evaluated after each phase. Overall, rotifers assimilated ARA in higher concentrations (23.3% of total fatty acids) than Artemia (0.6-0.7% of total fatty acids). Rotifers also tended to assimilate PUFAs better in EE form than in TG form (71.3% versus 66.5% Σn-3 + n-6 fatty acids). In the first experiment, fish from the ARA-and DHAenriched diet groups had greater swim bladder inflation rates (phase I) and growth rates (phase II) than the unenriched control group. In phase I of the second experiment, the EE enrichment group had a significantly faster growth rate than the TG enrichment group (specific growth rate: mean AE SD = 40.5 AE 0.9% and 36.6 AE 1.8%, respectively). Fatty acid composition of zooplankton was heavily influenced by enrichments, and fatty acid composition of larvae/ juveniles reflected that of their live prey. The results of this study suggest that PUFA enrichment of live feeds can accelerate Yellow Perch growth and reduce the time spent in the critical period of early development.