Effects of dietary 20-hydroxyecdysone supplementation on whole-body protein turnover in growing pigs

Abstract

One of the approaches to creating biologically active additives for use in pig breeding can be the use of 20-hydroxyecdysone regulating protein metabolism in piglets. The purpose of the work is to assess the effect of 20-hydroxyecdysone on turnover of protein in piglets. The experiment was carried out on barrows (♂ Danish Yorkshire × ♀ Danish landrace) to achieve a live weight of 53-62 kg. At the age of 60 days, 2 groups of piglets were formed: control and experimental. Piglets of the experimental group were injected with 20-hydroxyecdysone at a dose of 1.6 mg / kg body weight. In piglets of the experimental group, in comparison with the control, a decrease in the excretion of nitrogen in the urine was noted (by 26.8%, P <0.05). Nitrogen deposition was higher in piglets of the experimental group by 19.0% (P <0.001) compared with the control. 20-hydroxyecdysone contributed to increased protein deposition in the body of piglets due to protein synthesizing activity. Thus, the use of 20-hydroxyecdysone in pigs increases the efficiency of using amino acids for the synthesis and deposition of proteins in the body.

Effect of amino acid level and ratio on whole-body protein turnover in piglets

“…Most studies use the nitrogen metabolism model to measure protein turnover in the whole body [17], which is based on measuring the kinetics of metabolism of the introduced marker. To measure the rate of protein synthesis using this model, the total precursor flux, the rate of formation of the final products of nitrogen metabolism, and the rate of precursor secretion from the total fund into the gastrointestinal tract must be known.…”

“…To measure the rate of protein synthesis using this model, the total precursor flux, the rate of formation of the final products of nitrogen metabolism, and the rate of precursor secretion from the total fund into the gastrointestinal tract must be known. Methodological aspects of measuring the rates of protein synthesis and decomposition in the whole body using 15 N amino acids are considered in detail in [17]. The results obtained by the researchers indicate that the use of different amino acids gives comparable data, despite the fact that the specificity of these amino acids as precursors for protein synthesis is not the same.…”

“…During the balance experiment, the rate of synthesis, decomposition, and deposition of total body proteins was determined in piglets at the end of the growing period according to the method [17] using labeled amino acid for nitrogen 15 N-glycine. 15 N-glycine with an enrichment of 98 % atomic excess was administered per os in the amount of 3 mg 15 N per 1 kg of animal body weight for 7 days.…”