Effects of Diesel on Survival, Growth, and Gene Expression in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Fry

Mos, Lizzy; Cooper, Glenn A.; Serben, Kerrie Christine; Cameron, Marc; Koop, Ben F.

doi:10.1021/es702215c

Cited by 24 publications

(20 citation statements)

References 29 publications

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“…A reduction in oxygen transport driven by lowered hemoglobin expression was used to explain the behavior of oil-exposed O . mykiss “gulping” for air at the surface when oxygen levels were high [66]. We observed similar instances of oil-exposed flounder swimming to the surface in well-oxygenated tanks.…”

Section: Resultssupporting

confidence: 68%

“…European flounder ( Pleuronectes flesus ) exposed to the water-soluble fraction of crude oil exhibited decreased levels of hematocrit and hemoglobin [63]. Down-regulation of hemoglobin genes (including β-hemoglobin) has been demonstrated for Paralichthys olivaceous [36,64] and Oncorhyncus mykiss [65,66] in response to exposure to various oil types (heavy, diesel, kerosene, etc.) and individual PAHs.…”

Section: Resultsmentioning

confidence: 99%

“…and individual PAHs. β-hemoglobin down-regulation resulted in lowered erythrocyte levels, leading to a reduction in oxygen transport [36,64,66], which was observed when the cladoceran Daphnia magna was exposed to naphthalene [67]. A reduction in oxygen transport driven by lowered hemoglobin expression was used to explain the behavior of oil-exposed O .…”

Section: Resultsmentioning

confidence: 99%

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Crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder

et al. 2017

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Exposure to crude oil or its individual constituents can have detrimental impacts on fish species, including impairment of the immune response. Increased observations of skin lesions in northern Gulf of Mexico fish during the 2010 Deepwater Horizon oil spill indicated the possibility of oil-induced immunocompromisation resulting in bacterial or viral infection. This study used a full factorial design of oil exposure and bacterial challenge to examine how oil exposure impairs southern flounder (Paralichthys lethostigma) immune function and increases susceptibility to the bacteria Vibrio anguillarum, a causative agent of vibriosis. Fish exposed to oil prior to bacterial challenge exhibited 94.4% mortality within 48 hours of bacterial exposure. Flounder challenged with V. anguillarum without prior oil exposure had <10% mortality. Exposure resulted in taxonomically distinct gill and intestine bacterial communities. Mortality strongly correlated with V. anguillarum levels, where it comprised a significantly higher percentage of the microbiome in Oil/Pathogen challenged fish and was nearly non-existent in the No Oil/Pathogen challenged fish bacterial community. Elevated V. anguillarum levels were a direct result of oil exposure-induced immunosuppression. Oil-exposure reduced expression of immunoglobulin M, the major systemic fish antibody, and resulted in an overall downregulation in transcriptome response, particularly in genes related to immune function, response to stimulus and hemostasis. Ultimately, sediment-borne oil exposure impairs immune function, leading to increased incidences of bacterial infections. This type of sediment-borne exposure may result in long-term marine ecosystem effects, as oil-bound sediment in the northern Gulf of Mexico will likely remain a contamination source for years to come.

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Section: Resultssupporting

confidence: 68%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder

et al. 2017

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“…Twenty-five genes, including CYP1A, GST 3, hemoglobin beta chain, and glyceraldehyde-3-phosphate dehydrogenase, were in the gene expression profile following benzo[a]pyrene exposure [17] and following exposure to at least one oil in the present study (Supplemental Data, Table S11). Fish exposed to diesel fuel had changes in the transcript abundance of CYP1A, sulfotransferase 6B1, thioredoxin, GST, complement component C7, HSP 90, DNA-directed RNA polymerases I, II, and III, hemoglobin alpha and beta chains, glyceraldehyde-3-phosphate dehydrogenase, and hemopexin precursor [35]; these genes were altered in our present study as well. Finally, the kidneys of Japanese flounder exposed to heavy oil had changes in the transcript levels of hemoglobin beta chain, beta-2-microglobulin, and Gene expression in trout exposed to different hydrocarbons Environ.…”

Section: Discussionsupporting

confidence: 70%

Hepatic gene expression in rainbow trout (Oncorhynchus mykiss) exposed to different hydrocarbon mixtures

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Lampi

Febbo

et al. 2010

Enviro Toxic and Chemistry

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Traditional biomarkers for hydrocarbon exposure are not induced by all petroleum substances. The objective of this study was to determine if exposure to a crude oil and different refined oils would generate a common hydrocarbon-specific response in gene expression profiles that could be used as generic biomarkers of hydrocarbon exposure. Juvenile rainbow trout (Oncorhynchus mykiss) were exposed to the water accommodated fraction (WAF) of either kerosene, gas oil, heavy fuel oil, or crude oil for 96 h. Tissue was collected for RNA extraction and microarray analysis. Exposure to each WAF resulted in a different list of differentially regulated genes, with few genes in common across treatments. Exposure to crude oil WAF changed the expression of genes including cytochrome P4501A (CYP1A) and glutathione-S-transferase (GST) with known roles in detoxification pathways. These gene expression profiles were compared to others from previous experiments that used a diverse suite of toxicants. Clustering algorithms successfully identified gene expression profiles resulting from hydrocarbon exposure. These preliminary analyses highlight the difficulties of using single genes as diagnostic of petroleum hydrocarbon exposures. Further work is needed to determine if multivariate transcriptomic-based biomarkers may be a more effective tool than single gene studies for exposure monitoring of different oils.

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“…Cell-mediated immunity seems to be a sensitive endpoint in oiled birds, whereas antibody production appears less sensitive to oil exposure. Recent studies in both fish and mammals exposed to heavy petroleum show modulation of expression of immune system-related genes (Bowen et al 2007;Mos et al 2008;Nakayama et al 2008). Although petroleum and the PAH component of oils are known to affect the immune systems of aquatic organisms and wildlife, immunotoxicity is not typically assessed during oil spills.…”

Section: Implications For Immunotoxicitymentioning

confidence: 99%

Ecological Impacts of the Deepwater Horizon Oil Spill: Implications for Immunotoxicity

2011

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The Deepwater Horizon (DWH) oil spill was the largest environmental disaster and response effort in U.S. history, with nearly 800 million liters of crude oil spilled. Vast areas of the Gulf of Mexico were contaminated with oil, including deep-ocean communities and over 1,600 kilometers of shoreline. Multiple species of pelagic, tidal, and estuarine organisms; sea turtles; marine mammals; and birds were affected, and over 20 million hectares of the Gulf of Mexico were closed to fishing. Several large-scale field efforts were performed, including assessments of shoreline and wildlife oiling and of coastal waters and sediments. The assessment of injuries, damages, and restoration options for the DWH spill is ongoing. Although petroleum and the polycyclic aromatic hydrocarbon component of oils are known to affect the immune systems of aquatic organisms and wildlife, immunotoxicity is not typically assessed during oil spills and has not been a focus of the DHW assessment. The effects of oil spill contaminants on immune responses are variable and often exposure dependent, but immunotoxic effects seem likely from the DHW spill based on the reported effects of a variety of oils on both aquatic and wildlife species.

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Effects of Diesel on Survival, Growth, and Gene Expression in Rainbow Trout (Oncorhynchus mykiss) Fry

Cited by 24 publications

References 29 publications

Crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder

Crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder

Hepatic gene expression in rainbow trout (Oncorhynchus mykiss) exposed to different hydrocarbon mixtures

Ecological Impacts of the Deepwater Horizon Oil Spill: Implications for Immunotoxicity

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