Effects of dichlorobenzene on acetylcholine receptors in human neuroblastoma SH-SY5Y cells

Yan, Ren-Ming; Chiung, Yin-Mei; Pan, Chien‐Yuan; Liu, Jenn-Hwa; Liu, Pei-Shan

doi:10.1016/j.tox.2008.07.066

Cited by 10 publications

(7 citation statements)

References 37 publications

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“…Human neuroblastoma SH-SY5Y cells, obtained from ATCC (CRL-2266), were cultured as described previously [26], as were human osteosarcoma (HOS) cells, obtained from the Bioresource Collection and Research Center (Hsinchu, Taiwan) [27].…”

Section: Cell Culturementioning

confidence: 99%

Effects of Hericium erinaceus Mycelium Extracts on the Functional Activity of Purinoceptors and Neuropathic Pain in Mice with L5 Spinal Nerve Ligation

Yang

Chueh

Shie

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Neuropathic pain is a serious clinical problem that is difficult to treat. Purinoceptors (P2Rs) transduce pain perception from the peripheral to the central nervous system and play an important role in the transmission of neuropathic pain signals. We previously found that the crude extracts of Hericium erinaceus mycelium (HE-CE) inhibited P2R-mediated signaling in cells and reduced heat-induced pain in mice. The present study explored the effects of HE-CE on neuropathic pain. We used adenosine triphosphate (ATP) as a P2R agonist to generate Ca2+ signaling and neuronal damage in a cell line. We also established a neuropathic mouse model of L5 spinal nerve ligation (L5-SNL) to examine neuropathic pain and neuroinflammation. Neuropathic pain was recorded using the von Frey test. Neuroinflammation was evaluated based on immunohistofluorescence observation of glial fibrillary acidic protein (GFAP) levels in astrocytes, ionized calcium-binding adaptor molecule1 (iba1) levels in microglia, and IL-6 levels in plasma. The results show that HE-CE and erinacine-S, but not erinacine-A, totally counteracted Ca2+ signaling and cytotoxic effects upon P2R stimulation by ATP in human osteosarcoma HOS cells and human neuroblastoma SH-SY5Y cells, respectively. SNL induced a decrease in the withdrawal pressure of the ipsilateral hind paw, indicating neuropathic pain. It also raised the GFAP level in astrocytes, the iba1 level in microglia, and the IL-6 level in plasma, indicating neuroinflammation. HE-CE significantly counteracted the SNL-induced decrease in withdrawal pressure, illustrating that it could relieve neuropathic pain. It also reduced SNL-induced increases in astrocyte GFAP levels, microglial iba1 levels, and plasma IL-6 levels, suggesting that HE-CE reduces neuroinflammation. Erinacine-S relieved neuropathic pain better than HE-CE. The present study demonstrated that HE inhibits P2R and, thus, that it can relieve neuropathic pain and neuroinflammation.

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Section: Cell Culturementioning

confidence: 99%

Effects of Hericium erinaceus Mycelium Extracts on the Functional Activity of Purinoceptors and Neuropathic Pain in Mice with L5 Spinal Nerve Ligation

Yang

Chueh

Shie

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Yan and colleagues found that PDCB affects calcium homeostasis in neural tissue by inhibition of nicotinic acetylcholine receptor ion channels [Yan et al 2008]. Subsequently PDCB mediated alteration in intracellular and extracellular calcium ion concentration in vitro might obstruct neuronal signaling leading to neuronal toxicity.…”

Section: Mode Of Pdcb Toxicitymentioning

confidence: 99%

“…Yan and colleagues found that PDCB affects calcium homeostasis in neural tissue by inhibition of nicotinic acetylcholine receptor ion channels [Yan et al . 2008].…”

Section: Mode Of Pdcb Toxicitymentioning

confidence: 99%

Para-dichlorobenzene toxicity – a review of potential neurotoxic manifestations

Dubey

Sharma

Pass

et al. 2014

Ther Adv Neurol Disord

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“…Oral administration of a radioactively-labeled 1,2-DCB to rats revealed that it can be distributed among all tissues, including skeletal muscle. In addition, it has been shown that DCB molecules can induce a rise in intracellular Ca 2+ in human neuronal SH-SY5Y cells [ 7 , 8 ]. Given this evidence, we hypothesized that 1,2-DCB can affect the functionality of skeletal muscle proteins, particularly the Ca 2+ -ATPase from sarcoplasmic reticulum (SERCA), a predominant protein of skeletal muscle, a hypothesis that has not previously been explored.…”

Section: Introductionmentioning

confidence: 99%

1,2-Dichlorobenzene affects the formation of the phosphoenzyme stage during the catalytic cycle of the Ca2+-ATPase from sarcoplasmic reticulum

2016

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Background1,2-Dichlorobenzene (1,2-DCB) is a benzene-derived molecule with two Cl atoms that is commonly utilized in the synthesis of pesticides. 1,2-DCB can be absorbed by living creatures and its effects on naturally-occurring enzymatic systems, including the effects on Ca2+-ATPases, have been poorly studied. Therefore, we aimed to study the effect of 1,2-DCB on the Ca2+-ATPase from sarcoplasmic reticulum (SERCA), a critical regulator of intracellular Ca2+ concentration.ResultsConcentrations of 0.05–0.2 mM of 1,2-DCB were able to stimulate the hydrolytic activity of SERCA in a medium-containing Ca2+-ionophore. At higher concentrations (0.25–0.75 mM), 1,2-DCB inhibited the ATP hydrolysis to ~80 %. Moreover, ATP hydrolysis and Ca2+ uptake in a medium supported by K-oxalate showed that starting at 0.05 mM,1,2-DCB was able to uncouple the ratio of hydrolysis/Ca2+ transported. The effect of this compound on the integrity of the SR membrane loaded with Ca2+ remained unaffected. Finally, the analysis of phosphorylation of SERCA by [γ-32P]ATP, starting under different conditions at 0° or 25 °C showed a reduction in the phosphoenzyme levels by 1,2-DCB, mostly at 0 °C.ConclusionsThe temperature-dependent decreased levels of phosphoenzyme by 1,2-DCB could be due to the acceleration of the dephosphorylation mechanism – E2P · Ca2 state to E2 and Pi, which explains the uncoupling of the ATP hydrolysis from the Ca2+ transport.

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Effects of dichlorobenzene on acetylcholine receptors in human neuroblastoma SH-SY5Y cells

Cited by 10 publications

References 37 publications

Effects of Hericium erinaceus Mycelium Extracts on the Functional Activity of Purinoceptors and Neuropathic Pain in Mice with L5 Spinal Nerve Ligation

Effects of Hericium erinaceus Mycelium Extracts on the Functional Activity of Purinoceptors and Neuropathic Pain in Mice with L5 Spinal Nerve Ligation

Para-dichlorobenzene toxicity – a review of potential neurotoxic manifestations

1,2-Dichlorobenzene affects the formation of the phosphoenzyme stage during the catalytic cycle of the Ca2+-ATPase from sarcoplasmic reticulum

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