Effects of Dicer and Argonaute down-regulation on mRNA levels in human HEK293 cells

Schmitter, Daniela; Filkowski, Jody; Sewer, Alain; Pillai, Ramesh S.; Oakeley, Edward J.; Zavolan, Mihaela; Svoboda, Petr; Filipowicz, Witold

doi:10.1093/nar/gkl646

Cited by 184 publications

(186 citation statements)

References 51 publications

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“…However, an increasing body of evidence suggests that miRNA-mRNA interaction leads to a de-stabilization of mRNA leading to alteration in mRNA levels, albeit modest. Although enough evidence of alteration of transcript levels exists in microarray experiments, 18 the exact mechanism has only been partially elucidated in Drosophila melanogaster and zebrafish. 19,20 Experiments have shown that the P body protein GW 182 marks the mRNA for decay.…”

Section: Micrornas Regulate Gene Expressionmentioning

confidence: 99%

The role of microRNA in human leukemia: a review

Yendamuri

Calin

2009

Leukemia

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MicroRNAs (miRNAs or miRs) are 18-22-nucleotide non-coding RNAs that have emerged as a new paradigm of epigenetic regulation in both normal development and cellular function, and in the pathogenesis of human disease including cancer. This review summarizes the current literature of mechanism of gene regulation by miRNA and their role in hematopoiesis and leukemogenesis. An understanding of these processes suggests further avenues for research to understand gene regulation and miRNA-based therapeutic approaches.

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Section: Micrornas Regulate Gene Expressionmentioning

confidence: 99%

The role of microRNA in human leukemia: a review

Yendamuri

Calin

2009

Leukemia

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“…42 Reporters measuring activity of endogenous Let-7 and miR-30 showed that ability of miRNAs to repress translation is strongly reduced during oocyte growth and maturation while RNAi-like cleavage was less affected. Interestingly, the less abundant miR-30 apparently retained more silencing activity suggesting that additional mechanism(s) reduce Let-7 activity in the oocyte.…”

Section: Mammalian Mirna and Rnai Pathwaysmentioning

confidence: 99%

Why mouse oocytes and early embryos ignore miRNAs?

Svoboda¹

2010

RNA Biology

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Small RNA molecules regulating gene expression received a status of omnipresent master regulators of eukaryotic lives with almost supernatural powers. Mammals hold at least three mechanisms employing small RNA molecules for regulating gene expression. One of these mechanisms, the microRNA (miRNA) pathway, currently involves over a thousand of genome-encoded different miRNAs that are claimed to extend their control over more than a half of a genome. Here, I discuss how and why mouse oocytes and early embryos ignore the regulatory power of miRNAs, adding another surprising feature to the field of small RNAs.

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“…The miR-9500 is transcribed from an intergenic region and yields a 19 ntmature sequence. The gene encoding miR-9500 is located on chromosome 3, and the mature sequence is generated through dicer-dependent processing 31 ( Supplementary Figure 4). In addition, we found that miR-9500 was expressed in almost all cells (Supplementary Figure 5), and the mature sequence of miR-9500 is conserved in other mammals (Figure 1b).…”

Section: Discussionmentioning

confidence: 99%

The novel miR-9500 regulates the proliferation and migration of human lung cancer cells by targeting Akt1

Jung

Lee

et al. 2014

Cell Death Differ

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MicroRNAs have crucial roles in lung cancer cell development. They regulate cell growth, proliferation and migration by mediating the expression of tumor suppressor genes and oncogenes. We identified and characterized the novel miR-9500 in human lung cancer cells. The miR-9500 forms a stem-loop structure and is conserved in other mammals. The expression levels of miR-9500 were reduced in lung cancer cells and lung cancer tissues compared with normal tissues, as verified by TaqMan miRNA assays. It was confirmed that the putative target gene, Akt1, was directly suppressed by miR-9500, as demonstrated by a luciferase reporter assay. The miR-9500 significantly repressed the protein expression levels of Akt1, as demonstrated via western blot, but did not affect the corresponding mRNA levels. Akt1 has an important role in lung carcinogenesis, and depletion of Akt1 has been shown to have antiproliferative and anti-migratory effects in previous studies. In the current study, the overexpression of miR-9500 inhibited cell proliferation and the expression of cell cycle-related proteins. Likewise, the overexpression of miR-9500 impeded cell migration in human lung cancer cells. In an in vivo assay, miR-9500 significantly suppressed Fluc expression compared with NC and ASO-miR-9500, suggesting that cell proliferation was inhibited in nude mice. Likewise, miR-9500 repressed tumorigenesis and metastasis by targeting Akt1. These data indicate that miR-9500 might be applicable for lung cancer therapy.

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Effects of Dicer and Argonaute down-regulation on mRNA levels in human HEK293 cells

Cited by 184 publications

References 51 publications

The role of microRNA in human leukemia: a review

The role of microRNA in human leukemia: a review

Why mouse oocytes and early embryos ignore miRNAs?

The novel miR-9500 regulates the proliferation and migration of human lung cancer cells by targeting Akt1

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