Effects of diagnostic ultrasound-targeted microbubble destruction on permeability of normal liver in rats

Yang, Dan; Tan, Kaibin; Gao, Yunhua; Liu, Hong; Yang, Wei

doi:10.1016/j.ultras.2012.09.002

Cited by 15 publications

(31 citation statements)

References 24 publications

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“…Our GE Vivid 7 Dimension machine was used with our replacement contrast agent (similar to Definity). Results were generally supportive of the study by Yang et al and also indicated hepatocyte injury by Evans blue staining after application of intermittent exposure protocols similar to our previous heart and kidney research.…”

supporting

confidence: 91%

“…Yang et al used a GE Vivid 7 Dimension machine with an S4 transducer placed on the abdomen. The second harmonic B‐mode was used with 1.5‐MHz transmit and 3.2‐MHz receive frequencies.…”

Section: Methodsmentioning

confidence: 99%

“…The mean diameter was slightly larger at 1.8 ± 0.11 μm diameter than actual Definity microbubbles at 1.6 ± 0.04 μm, but the concentration of microbubbles in our tests was not substantially different from that of Definity at 3.7 ± 0.3 10 9 mL –1 . The replacement contrast agent was prepared each day, diluted with sterile saline, and administered as a bolus dose of 500 μL/kg to reproduce the dosage used by Yang et al or as an infusion of 50 μL/kg/min for the 10‐minute scans. Bolus injection was used for contact exposures.…”

Section: Methodsmentioning

confidence: 99%

“…Yang et al used a Vivid 7 Dimension US machine (GE Vingmed Ultrasound, Horten, Norway) with an S4 transducer at 1.5 MHz to evaluate the safety of US‐targeted microbubble destruction (related to use of diagnostic US as a means for gene delivery to liver cells). The probe was handheld on the depilated abdomens of rats with a 3‐cm image depth.…”

mentioning

confidence: 99%

“…The purpose of this study was to evaluate CEDUS‐induced bioeffects in rat liver. The study by Yang et al was used as the initial model because it appears to be the only account of CEDUS‐induced liver injury. Our GE Vivid 7 Dimension machine was used with our replacement contrast agent (similar to Definity).…”

mentioning

confidence: 99%

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Hepatocyte Injury Induced by Contrast‐Enhanced Diagnostic Ultrasound

Miller

Fabiilli

et al. 2018

J of Ultrasound Medicine

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Objectives-Contrast-enhanced diagnostic ultrasound (US) has a potential to induce localized biological effects. The potential for contrast-enhanced diagnostic US bioeffects in liver were researched, with guidance from a report by Yang et al (Ultrasonics 2012; 52:1065-1071.Methods-Contact and standoff scanning was performed for 10 minutes with a diagnostic US phased array at 1.6 MHz during bolus injection or infusion of a contrast agent at a high dose. The impact of the imaging on rat liver was investigated by measuring enzyme release, microvascular leakage, and staining of injured hepatocytes.Results-The results showed liver enzyme release at 30 minutes, indicating liver injury, and elevated extraction of Evans blue dye, indicating microvascular leakage. In addition, Evans blue and trypan blue vital-staining methods revealed scattered stained cells within the US scan plane. For the Evans blue method, fluorescent cell counts in frozen sections were greatest for standoff exposure with contrast infusion. The count decreased strongly with depth for bolus injection, which was probably reflective of the high attenuation noted for this agent delivery method. Conclusions-The results qualitatively confirmed the report by Yang et al and additionally showed hepatocyte vital staining. Research is needed to determine the threshold for the effects and the contrast agent dose response.

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supporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Hepatocyte Injury Induced by Contrast‐Enhanced Diagnostic Ultrasound

Miller

Fabiilli

et al. 2018

J of Ultrasound Medicine

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Ultrasound‐targeted microbubble enhances migration and therapeutic efficacy of marrow mesenchymal stem cell on rat middle cerebral artery occlusion stroke model

Qian

Wang

et al. 2018

J of Cellular Biochemistry

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To investigate the role of ultrasound-targeted microbubbles in the homing effect of bone marrow-derived mesenchymal stem cells (BMSCs) and in the therapeutic efficacy of BMSCs on the ischemic stroke. A middle cerebral artery occlusion (MCAO) model was induced by plug wire preparation. Seventy-two hours after MCAO, the treatment of BMSCs with ultrasound-targeted microbubble was assessed via modified neurological severity score (mNSS), infarct volumes, and cerebral edema. In addition, immunofluorescence was performed to analyze the homing effect of BMSCs with ultrasound-targeted microbubble. We find that BMSCs with ultrasound-targeted microbubble (BMMSCs with ultrasound-targeted microbubble [USMM] group) could significantly ameliorate mNSS, infarct volumes, and cerebral J Cell Biochem. 2019;120:3315-3322.wileyonlinelibrary.com/journal/jcb

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