Ultrasound‐targeted microbubble enhances migration and therapeutic efficacy of marrow mesenchymal stem cell on rat middle cerebral artery occlusion stroke model

Qian, Jian; Wang, Luna; Li, Qi Ming; Sha, Dujuan; Wang, Jun; Zhang, Jun; Xu, Peng; Fan, Guo-Chang

doi:10.1002/jcb.27600

Cited by 11 publications

(8 citation statements)

References 38 publications

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“…UTMd is combined with BMSc transplantation, there is a beneficial effect on the engraftment of cells (52,53). Ultrasound microbubbles can enhance the therapeutic effect of BMScs in diseases and promote BMSc homing (51,54,55). In the present study, the BUTMd + siRNA Nc group had an intermediate therapeutic effect.…”

Section: Discussionmentioning

confidence: 45%

Ultrasound‑targeted microbubble destruction‑mediated Galectin‑7‑siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats

et al. 2020

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Bone marrow mesenchymal stem cells (BMSCs) are accepted as a form of cellular therapy to improve cardiac function following acute myocardial infarction (AMI). The present study was performed to investigate the synergistic effect of ultrasound-targeted microbubble destruction (UTMD)-mediated Galectin-7-small interfering (si)RNA with the homing of BMSCs for AMI. The rat model of AMI was established, followed by identification of BMSCs. Rats with AMI received BMSC transplantation, BMSC transplantation + UTMD + siRNA negative control, or BMSC transplantation + UTMD + Galectin-7-siRNA. The cardiac function, hemodynamics indexes, degree of myocardial fiber injury and expression of apoptosis-related proteins in myocardial tissues of rats were detected. The homing of BMSCs was observed, and the indexes of myocardial microenvironment and the TGF-β/Smads pathway-related proteins in myocardial tissues were determined. AMI rats treated with UTMD-mediated Galectin-7-siRNA exhibited improved cardiac function and hemodynamics-related indices, decreased myocardial fiber injury and apoptotic cells, as well as enhanced homing ability of BMSCs, improved myocardial microenvironment, and suppressed TGF-β1/Smads pathway activation. In conclusion, the present study demonstrated that UTMD-mediated Galectin-7-siRNA treatment could enhance the homing ability of BMSCs, thus alleviating AMI in rats.

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Section: Discussionmentioning

confidence: 45%

Ultrasound‑targeted microbubble destruction‑mediated Galectin‑7‑siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats

et al. 2020

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“…BMSCs cells were made to express GFP protein which was expressed higher in the USSM group. This indicates that ultrasound-targeted microbubbles reduce cerebral edema and cerebral infarction volume, enhance the homing effect of vein transplanted BMSCs, improve the neurological function score, and thus enhance the effect of stem cell transplantation treating ischemic stroke …”

Section: Applications Of Ultrasound In Stem Cells Engineering and Reg...mentioning

confidence: 92%

“…This indicates that ultrasound-targeted microbubbles reduce cerebral edema and cerebral infarction volume, enhance the homing effect of vein transplanted BMSCs, improve the neurological function score, and thus enhance the effect of stem cell transplantation treating ischemic stroke. 134 An acousto-fluidic mediated approach was used to deliver plasmids in the human hematopoietic stem cells and progenitor cells. This method enabled pore formation and permeabilization of cell membranes under the effect of acoustic waves.…”

Section: Applications Of Ultrasound In Stem Cellsmentioning

confidence: 99%

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Bansal

Jha

Bhatia

et al. 2021

ACS Biomater. Sci. Eng.

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The role of ultrasound in medicine and biological sciences is expanding rapidly beyond its use in conventional diagnostic imaging. Numerous studies have reported the effects of ultrasound on cellular and tissue physiology. Advances in instrumentation and electronics have enabled successful in vivo applications of therapeutic ultrasound. Despite path breaking advances in understanding the biophysical and biological mechanisms at both microscopic and macroscopic scales, there remain substantial gaps. With the progression of research in this area, it is important to take stock of the current understanding of the field and to highlight important areas for future work. We present herein key developments in the biological applications of ultrasound especially in the context of nanoparticle delivery, drug delivery, and regenerative medicine. We conclude with a brief perspective on the current promise, limitations, and future directions for interfacing ultrasound technology with biological systems, which could provide guidance for future investigations in this interdisciplinary area.

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“…Ultrasound-targeted microbubble destruction can disrupt biological barriers and increase vascular permeability, including the blood-brain barrier (BBB) [ 45 ]; this technique may help more transplanted cells to migrate to the injured brain area. Qian et al found that ultrasound-targeted microbubbles enhanced the homing effect of BMSCs during the application of transplantation to treat ischemic stroke [ 46 ]. This improved level of homing led to the amelioration of cerebral edema, a reduction in infarct size, and an improved neurological function score in a rat model of MCAO.…”

Section: Physical Approaches To Improve the Efficiency Of Msc Transplantationmentioning

confidence: 99%

Strategies to Improve the Efficiency of Transplantation with Mesenchymal Stem Cells for the Treatment of Ischemic Stroke: A Review of Recent Progress

Wang

Tang

2021

Stem Cells International

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Cerebral ischemia is a common global disease that is characterized by a loss of neurological function and a poor prognosis in many patients. However, only a limited number of treatments are available for this condition at present. Given that the efficacies of these treatments tend to be poor, cerebral ischemia can create a significant burden on patients, families, and society. Mesenchymal stem cell (MSC) transplantation treatment has shown significant potential in animal models of ischemic stroke; however, the specific mechanisms underlying this effect have yet to be elucidated. Furthermore, clinical trials have yet to yield promising results. Consequently, there is an urgent need to identify new methods to improve the efficiency of MSC transplantation as an optimal treatment for ischemic stroke. In this review, we provide an overview of recent scientific reports concerning novel strategies that promote MSC transplantation as an effective therapeutic approach, including physical approaches, chemical agents, traditional Chinese medicines and extracts, and genetic modification. Our analyses showed that two key factors need to be considered if we are to improve the efficacy of MSC transplantation treatments: survival ability and homing ability. We also highlight the importance of other significant mechanisms, including the enhanced activation of MSCs to promote neurogenesis and angiogenesis, and the regulation of permeability in the blood-brain barrier. Further in-depth investigations of the specific mechanisms underlying MSC transplantation treatment will help us to identify effective methods that improve the efficiency of MSC transplantation for ischemic stroke. The development of safer and more effective methods will facilitate the application of MSC transplantation as a promising adjuvant therapy for the treatment of poststroke brain damage.

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Ultrasound‐targeted microbubble enhances migration and therapeutic efficacy of marrow mesenchymal stem cell on rat middle cerebral artery occlusion stroke model

Cited by 11 publications

References 38 publications

Ultrasound‑targeted microbubble destruction‑mediated Galectin‑7‑siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats

Ultrasound‑targeted microbubble destruction‑mediated Galectin‑7‑siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Strategies to Improve the Efficiency of Transplantation with Mesenchymal Stem Cells for the Treatment of Ischemic Stroke: A Review of Recent Progress

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