Effects of diabetes and vascular occlusion on adenosine-induced relaxant response of rat common carotid artery

Abstract

Adenosine produced equi-effective endothelium-independent relaxation of CA in all groups. Pharmacological potency of adenosine was reduced in diabetic animals solely, but even more in diabetic rats submitted to CA occlusion. The enhanced potassium transmembrane flow has certain protective role on adenosine-induced action in occluded CA from non-diabetic rats. Conversely, diabetes solely inhibited adenosine-evoked cascade connected to increased potassium conductance.

Clarification of serotonin‐induced effects in peripheral artery disease observed through the femoral artery response in models of diabetes and vascular occlusion: The role of calcium ions

“…Serotonin (0.001-10 μmol/L) produced comparable concentrationdependent contractions in the femoral arteries obtained from healthy animals ( Figure 2, Table 1) and sham-operated rats in both endothelium-preserved (E max =199.3±15.25; pEC 50 =7006±0.071; AUC=379.2±47.67) and denuded (E max =187.55±11.72; pEC 50 =7.03±0.04; AUC=382.1±41.79) blood vessels. Considering "The 3Rs" principle [10][11][12] and in order to avoid the further unnecessary use of experimental animals, the control group consisted of only healthy animals. 12 This was feasible since we did not notice any significant difference between the serotonin-induced contraction in healthy and sham-operated rats.…”

“…Considering "The 3Rs" principle [10][11][12] and in order to avoid the further unnecessary use of experimental animals, the control group consisted of only healthy animals. 12 This was feasible since we did not notice any significant difference between the serotonin-induced contraction in healthy and sham-operated rats.…”

“…Experimental animals were randomly divided into two main groups – the first without diabetes and the second with alloxan‐induced diabetes . These groups were further divided into two clusters: non‐operated animals and operated animals that were submitted to femoral artery occlusion.…”

“…Before beginning the surgical procedure, the animals were left to stabilize for a period of 90 minutes. After this period, the animals were opened, and the beginning of the left and right femoral arteries were occluded with a cord for a period of 45 . To test the success of the femoral artery occlusion, an additional observation of change in temperature and colour of rat paw was performed.…”

“…Experimental animals were randomly divided into two main groups -the first without diabetes and the second with alloxan-induced diabetes. 12 These groups were further divided into two clusters: nonoperated animals and operated animals that were submitted to femoral artery occlusion. Additionally, the shame-operated cluster was introduced into each group in order to determine the possible influence of the surgical procedure on the obtained results.…”