Effects of denervation on the glycogen content and on the activities of enzymes of glucose and glycogen metabolism in rat diaphragm muscle

Turner, L. V.; Manchester, Keith

doi:10.1042/bj1280789

Cited by 37 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transient hypertrophy of the hemidiaphragm after denervation has been previously observed. 20 Amounts of phosphorylase were determined in extracts of the 3 muscles after quantitative immunoprecipitation (Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

Phosphorylase kinase isozymes and phosphorylase in denervated skeletal muscles

Lawrence

Smith

1990

Muscle and Nerve

View full text Add to dashboard Cite

The effects of motor denervation on levels of phosphorylase kinase isozymes and phosphorylase were investigated in rat epitrochlearis, hemidiaphragm, and soleus muscles. Amounts of the proteins were measured after quantitative immunoprecipitation and found to be decreased by as much as 70% 2 weeks after denervation. Unexpectedly, denervation had little, if any, effect on the relative proportions of the two phosphorylase kinase isozymes. Phosphorylase and phosphorylase kinase were decreased by essentially the same extent after denervation, and the effects of denervation were comparable in all three muscles. The decreases in these enzymes explain, at least in part, the marked alterations in glycogen metabolism that occur after motor denervation.

show abstract

Section: Resultsmentioning

confidence: 99%

Phosphorylase kinase isozymes and phosphorylase in denervated skeletal muscles

Lawrence

Smith

1990

Muscle and Nerve

View full text Add to dashboard Cite

show abstract

“…Denervation of skeletal muscle results in a marked decrease in phosphorylase levels (Turner & Manchester, 1972;Butler et al, 1985a). In denervated chick breast muscle the levels of several glycolytic enzymes, including phosphorylase, decreased by 50 % compared with the innervated control; this new steady state was established within 2 weeks of nerve section (Shackleford & Lebherz, 1981).…”

Section: Introductionmentioning

confidence: 99%

Effect of denervation on the expression of glycogen phosphorylase in mouse skeletal muscle

Leyland

Turner

Beynon

1990

Biochemical Journal

View full text Add to dashboard Cite

After sciatectomy of the left hind-limb of C57BL/J mice, a denervation-induced muscular atrophy ensued and was accompanied by a decrease in the specific activity of glycogen phosphorylase to approx. 25 % of control values. The cofactor of phosphorylase, pyridoxal 5'-phosphate, was used as a specific label in the determination of the degradation rate of the enzyme following nerve section. After a delay of 3-4 days, phosphorylase was degraded approx. twice as rapidly in the denervated gastrocnemius (0.20 day-') as in the control muscle (0.12 day-'). The effect of denervation on phosphorylase mRNA was measured by quantitative Northern-blot analysis using a rat skeletal-muscle phosphorylase cDNA probe. After an initial rapid decline, phosphorylase mRNA levels stabilized in denervated muscle at 50 % of the value measured in the contralateral control muscle.

show abstract

“…Glycogen is an important nutrient reserve in animal tissue and it is used as an immediate energy source when required by any animal. Glycogen is an essential component of the normal metabolism (Thunberg and Manchester 1972). Many authors have reported that glycogen content can be affected in the pesticide-exposed insect.…”

Section: Discussionmentioning

confidence: 99%

Cellular energy allocation in the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae), following sublethal exposure to diazinon, fenitrothion, and chlorpyrifos

Chitgar¹,

Hajizadeh²,

Ghadamyari³

et al. 2014

Journal of Plant Protection Research

View full text Add to dashboard Cite

Abstract:It is necessary to study the biochemical changes in insects exposed to toxicants if we want to predict the potential of various chemicals on the natural enemy. Physiological energy, as a biochemical biomarker, may be affected by many pesticides including organophosphate compounds. Therefore, in this study, the sublethal effects of diazinon, fenitrothion, and chlorpyrifos on the cellular energy allocation (CEA) of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae), a potential biological control agent, was studied on 5th-instar nymphs. Among the energy reserves of the A. spinidens nymphs, only total protein was significantly affected by pesticide treatments, and the highest value was observed in chlorpyrifos treatment. The energy available (E a ) and energy consumption (E c ) in A. spinidens were significantly affected by these pesticides. In exposed bugs, these parameters were affected by fenitrothion and chlorpyrifos more than diazinon. The activity of the electron transport system (ETS) in the Ec assay showed that A. spinidens exposed to chlorpyrifos had the highest rate of oxygen consumption. Although, there was no significant change in CEA, the insecticides caused a marked change in the physiological balance of A. spinidens. The results suggested that the adverse effect of these insecticides on A. spinidens should be considered in Integrated Pest Management (IPM) programs.

show abstract

Effects of denervation on the glycogen content and on the activities of enzymes of glucose and glycogen metabolism in rat diaphragm muscle

Cited by 37 publications

References 36 publications

Phosphorylase kinase isozymes and phosphorylase in denervated skeletal muscles

Phosphorylase kinase isozymes and phosphorylase in denervated skeletal muscles

Effect of denervation on the expression of glycogen phosphorylase in mouse skeletal muscle

Cellular energy allocation in the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae), following sublethal exposure to diazinon, fenitrothion, and chlorpyrifos

Contact Info

Product

Resources

About