Cellular energy allocation in the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae), following sublethal exposure to diazinon, fenitrothion, and chlorpyrifos

Abstract

Abstract:It is necessary to study the biochemical changes in insects exposed to toxicants if we want to predict the potential of various chemicals on the natural enemy. Physiological energy, as a biochemical biomarker, may be affected by many pesticides including organophosphate compounds. Therefore, in this study, the sublethal effects of diazinon, fenitrothion, and chlorpyrifos on the cellular energy allocation (CEA) of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae), a potential biological control agent, was studied on 5th-instar nymphs. Among the energy reserves of the A. spinidens nymphs, only total protein was significantly affected by pesticide treatments, and the highest value was observed in chlorpyrifos treatment. The energy available (E a ) and energy consumption (E c ) in A. spinidens were significantly affected by these pesticides. In exposed bugs, these parameters were affected by fenitrothion and chlorpyrifos more than diazinon. The activity of the electron transport system (ETS) in the Ec assay showed that A. spinidens exposed to chlorpyrifos had the highest rate of oxygen consumption. Although, there was no significant change in CEA, the insecticides caused a marked change in the physiological balance of A. spinidens. The results suggested that the adverse effect of these insecticides on A. spinidens should be considered in Integrated Pest Management (IPM) programs.

Purification and characterization of the cuticle-degrading proteases produced by an isolate of Beauveria bassiana using the cuticle of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae)

“…Understanding the potential adaptability of the two biocontrol agents is crucial to preventing conflicts in an agro-ecosystem. Therefore, we are interested in the virulence of two isolates of B. bassiana -BB2 and AM-118 -on A. spinidens (Safavi 2010;Chitgar et al 2014;Firouzbakht et al 2015). In this context, we have conducted the current study by extracting and purifying Pr1 and Pr2 proteases produced by isolate AM-118.…”

Journal of Plant Protection Research

“…Analysis of the age-specific survival rate and the age-specific number of progeny per day indicated a significantly lower survival rate in the treated individuals. The insecticides made changes in cellular energy allocation of A. spinidens (Gholamzadeh-Chitgar et al 2014). Although no changes were reported in the amounts of total carbohydrates and glycogen within the treated nymphs, the amount of total protein increased in the treated nymphs compared to the control mainly following treatment with chlorpyrifos.…”

“…Gholamzadeh-Chitgar and colleagues designed a series of studies to determine the compatibility of A. spinidens with conventional insecticides sprayed on rice fields and the entomopathogenic fungus Beauveria bassiana Vuillemin (Gholamzadeh-Chitgar et at. 2014.…”

Comparative phosphoproteomics analysis provides insights into the responses of Chilo suppressalis to sublethal chlorantraniliprole exposure

“…35,36 Several studies revealed that sublethal insecticide exposure changed the metabolite contents (such as glycogen, trehalose, glucose, lipids and proteins) and the activities of enzymes (such as ⊍-amylase, trehalase, trypsin, lipase and protease) involved in carbohydrate and lipid metabolisms, leading to the nutrient metabolism dysfunction in various insects. [37][38][39][40][41][42][43][44][45][46][47] Specifically, sublethal chlorantraniliprole exposure significantly reduced the activities of digestive enzymes and resulted in the decreased food utilization and nutrient contents in Agrotis ipsilon and Pleonomus canaliculatus. 48,49 Chlorantraniliprole treatment also significantly decreased the activities of ⊍-amylase and trypsin in the midgut of Bombyx mori.…”