Effects of denervation and direct electrical stimulation upon the post-hatching differentiation of posterior latissimus dorsi muscle in chicken

Khaskiye, Ahmad; Renaud, D; Douarin, G.H. Le

doi:10.1016/0045-6039(86)90032-1

Cited by 7 publications

(6 citation statements)

References 33 publications

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“…These results roughly agree with the data obtained from cat soleus by HENRIKSSON et al (1982). Greater decrease in oxidative enzyme after denervation was also reported elsewhere (NEMETH, 1982;KHASKIYE et al, 1986).…”

Section: Discussionsupporting

confidence: 93%

Effects of denervation and deafferentation on mass and enzyme activity in rat skeletal muscles.

Ohira

1989

Jpn.J.Physiol

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Effects of denervation and deafferentation on mass and enzyme activity of soleus and plantaris muscles were studied when the synergistic gastrocnemius was either intact or tenotomized in adult male Wistar rats. Denervation of the sciatic nerve at L4 and L5 induced a severe atrophy (approximately 50%) in both soleus and plantaris within 2 weeks. Deafferentation at L4 and L5 also caused a decrease in muscle weight for soleus and plantaris (approximately 11 and 24%, respectively). No compensatory response to the tenotomy was induced either for muscle weight or enzyme activity in both denervated and deafferentated muscles. It is suggested that the efferent information(s) through the nerve fibers has an important effect of the regulation of both muscle mass and enzyme activity. Its effect on the activities was greater in mitochondrial than cytoplasmic enzymes. The data also suggest that the afferent information(s) plays a significant role for the regulation of muscle mass, including an induction of compensatory hypertrophy, but not for enzyme activity.

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Section: Discussionsupporting

confidence: 93%

Effects of denervation and deafferentation on mass and enzyme activity in rat skeletal muscles.

Ohira

1989

Jpn.J.Physiol

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“…For example, the ,o300 slow-staining fibers present in the 21-d fetal EDL are depleted by >60% as the animal matures (Table I). This result is consistent with findings in the mouse (48) and the chicken (22) and illustrates the plasticity of myosin expression as specialization advances in fast muscles. Thus, as the hind limb of the adolescent rat is drawn under the animal to assume an adult posture, expression of slow myosin appears to become less and less of a requirement for the tasks these fast muscles must perform, and our results suggest that slow, type I fibers in the adult EDL and AT may be viewed as relics of early development (see Figs.…”

Section: Discussionsupporting

confidence: 91%

Slow myosin in developing rat skeletal muscle.

Narusawa¹,

Fitzsimons²,

Izumo³

et al. 1987

The Journal of Cell Biology

245

135

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Abstract. Through S1 nuclease mapping using a specific cDNA probe, we demonstrate that the slow myosin heavy-chain (MHC) gene, characteristic of adult soleus, is expressed in bulk hind limb muscle obtained from the 18-d rat fetus. We support these results by use of a monoclonal antibody (mAb) which is highly specific to the adult slow MHC. Immunoblots of MHC peptide maps show the same peptides, uniquely recognized by this antibody in adult soleus, are also identified in 18-d fetal limb muscle. Thus synthesis of slow myosin is an early event in skeletal myogenesis and is expressed concurrently with embryonic myosin.By immunofluorescence we demonstrate that in the 16-d fetus all primary myotubes in future fast and future slow muscles homogeneously express slow as well as embryonic myosin. Fiber heterogeneity arises owing to a developmentally regulated inhibition of slow MHC accumulation as muscles are progressively assembled from successive orders of cells. Assembly involves addition of new, superficial areas of the anterior tibial muscle (AT) and extensor digitorum longus muscle (EDL) in which primary cells initially stain weakly or are unstained with the slow mAb. In the developing AT and EDL, expression of slow myosin is unstable and is progressively restricted as these muscles specialize more and more towards the fast phenotype. Slow fibers persisting in deep portions of the adult EDL and AT are interpreted as vestiges of the original muscle primordium.A comparable inhibition of slow MHC accumulation occurs in the developing soleus but involves secondary, not primary, cells. Our results show that the fate of secondary cells is flexible and is spatially determined. By RIA we show that the relative proportions of slow MHC are fivefold greater in the soleus than in the EDL or AT at birth. After neonatal denervation, concentrations of slow MHC in the soleus rapidly decline, and we hypothesize that, in this muscle, the nerve protects and amplifies initial programs of slow MHC synthesis. Conversely, the content of slow MHC rises in the neonatally denervated EDL. This suggests that as the nerve amplifies fast MHC accumulation in the developing EDL, accumulation of slow MHC is inhibited in an antithetic fashion.Studies with phenylthiouracil-induced hypothyroidism indicate that inhibition of slow MHC accumulation in the EDL and AT is not initially under thyroid regulation. At later stages, the development of thyroid function plays a role in inhibiting slow MHC accumulation in the differentiating EDL and AT. The effects of the nerve and of thyroid hormone on these developing fast muscles therefore appear synergistic. In the adult AT and EDL, hypothyroidism causes a significant rise in proportions of slow MHC, which selectively accumulates in type Ha and not IIb fibers. This pattern of accumulation is not a simple recapitulation of early programs of slow MHC expression.

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“…This hypotrophy of denervated-stimulated muscles may be related to the stimulation regime. For example, a 0.15-Hz rhythm of stimulation reduces denervation-induced hypotrophy in PLD muscle from 10-day-old chicken [18], and in rabbit EDL muscle, 1 -Hz stimulation retards denervation-induced atrophy; in contrast, tetanic stimulation (40 Hz) induces severe fibrosis and is harmful to denervated muscle [32].…”

Section: Discussionmentioning

confidence: 99%

“…At day 2 after hatching, unilateral (left) denervation of ALD and PLD muscles was carried out aseptically using chloroform anaesthesia. A 5to 8-mm segment was removed from the common nerve trunk supplying the latissimus dorsii muscles [18]. Chickens were allowed to survive until day 4, 10, 14, or 18 according to the experimental design ( Fig.…”

Section: Animalsmentioning

confidence: 99%

Posthatching changes in levels and molecular forms of acetylcholinesterase in slow and fast muscles of the chicken: Effects of denervation and direct electrical stimulation

Khaskiye¹,

Renaud²

1988

Differentiation

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Effects of denervation and direct electrical stimulation upon the post-hatching differentiation of posterior latissimus dorsi muscle in chicken

Cited by 7 publications

References 33 publications

Effects of denervation and deafferentation on mass and enzyme activity in rat skeletal muscles.

Effects of denervation and deafferentation on mass and enzyme activity in rat skeletal muscles.

Slow myosin in developing rat skeletal muscle.

Posthatching changes in levels and molecular forms of acetylcholinesterase in slow and fast muscles of the chicken: Effects of denervation and direct electrical stimulation

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