Posthatching changes in levels and molecular forms of acetylcholinesterase in slow and fast muscles of the chicken: Effects of denervation and direct electrical stimulation

Khaskiye, Ahmad; Renaud, D

doi:10.1111/j.1432-0436.1988.tb00077.x

Cited by 6 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4), suggests that DE-HSS 16s AChE conversion may require muscle membrane depolarization-induced Ca2 + influxes, but not the physical presence of (or putative trophic factors from) motor axons. This idea is supported by recent studies demonstrating that direct stimulation of curarized (Khaskiye et al, 1989) and denervated (Khaskiye, 1986;Khaskiye and Renaud, 1988) muscle reverses the effects of inactivity and denervation on 16s AChE accumulation. It is known that dysgenic muscle lacks voltage-sensitive Ca2+ channels (Tanabe et al, 1988;Rieger et al, 1987) probably as a result of a primary defect of the a, subunit of Ca2+ channels (Tanabe et al, 1988).…”

Section: Discussionmentioning

confidence: 54%

Developmental modulation of physicochemical variants of the tailed asymmetric (16S) acetylcholinesterase by neuromuscular activity and innervation in the mouse embryo

Houenou

Garcı́a

Verdière‐Sahuqué

et al. 1991

J of Neuroscience Research

View full text Add to dashboard Cite

We have studied the physicochemical properties of acetylcholinesterase (AChE) during embryonic development of normal and functionally impaired mouse skeletal muscle, focusing on the tailed asymmetric (16S) form of the enzyme. The muscle-specific 16S AChE exists in two different variants. One is associated with extracellular matrix and is high-salt soluble (HSS, also termed hydrophilic AChE), whereas the other form is anchored to cell membranes and is detergent extractable (DE, or hydrophobic AChE). Before innervation during normal embryonic development, both hydrophilic and hydrophobic 16S AChE exist in equal amounts. After muscle innervation, there was an increase (amounting three-fold on E18) in the levels of hydrophilic vs. hydrophobic 16S AChE. This alteration of the relative proportions of the two variants of 16S AChE did not occur in chronically inactive muscles either from the mouse mutant, muscular dysgenesis, or from tetrodotoxin-treated mouse embryos. Taken together with previous reports, the present results suggest that postsynaptic membrane depolarization-induced Ca2+ fluxes are important in modulating not only the synthesis of 16S AChE, but also the relative proportions of both physicochemical variants of this molecular form of AChE.

show abstract

Section: Discussionmentioning

confidence: 54%

Developmental modulation of physicochemical variants of the tailed asymmetric (16S) acetylcholinesterase by neuromuscular activity and innervation in the mouse embryo

Houenou

Garcı́a

Verdière‐Sahuqué

et al. 1991

J of Neuroscience Research

View full text Add to dashboard Cite

show abstract

“…Nevertheless, on maturation of chicken fast PLD muscle, the AChE A12 form remains predominant, whereas the BuChE AI2 form decreases rapidly Jedrzejczyk et al, 198 1). Moreover, in denervated-stimulated muscle, we have reported that AChE molecular forms are related to the rhythm of stimulation (Khaskiye and Renaud, 1988). The present work shows that this relationship cannot be extended to BuChE, because the regulation of its molecular forms appears to depend on the amount of stimuli.…”

Section: Jmentioning

confidence: 99%

“…In order to extend our previous results (Khaskiye and Renaud, 1988) and determine whether or not both AChE and BuChE are regulated by evoked muscle activity, we have analyzed the distribution of BuChE molecular forms in denervated PLD and ALD chicken muscles which were submitted to different patterns of electrical stimulation. The present results show that evoked muscle activity is strongly involved in the expression of BuChE asymmetric forms, mainly in fast PLD muscle.…”

mentioning

confidence: 97%

“…In addition, L0mo et al (1985) have reported that electrical stimulation of denervated rat soleus muscle with fast and slow activity patterns induces different expression of AChE molecular forms according to the frequency of evoked muscle activity. We have observed recently that, in denervated PLD muscle from newly hatched chickens, contractile activity elicited by direct electrical stimulation partly prevents the loss of synaptic AChE accumulation (Khaskiye, 1986) and of asymmetric forms and depresses the nse in AChE specific activity (Khaskiye and Renaud, 1988).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effects of Electrical Stimulation on Molecular Forms of Butyrylcholinesterase in Denervated Fast and Slow Latissimus Dorsi Muscles of Newly Hatched Chicken

Khaskiye¹,

Sine²,

Colas³

et al. 1990

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

The effects of denervation and direct electrical stimulation upon the activity and the molecular form distribution of butyrylcholinesterase (BuChE) were studied in fast-twitch posterior latissimus dorsi (PLD) and in slow-tonic anterior latissimus dorsi (ALD) muscles of newly hatched chicken. In PLD muscle, denervation performed at day 2 substantially reduced the rate of rapid decrease of BuChE specific activity which takes place during normal development, whereas in the case of ALD muscle little change was observed. Moreover, the asymmetric forms which were dramatically reduced in denervated PLD muscle were virtually absent in denervated ALD muscle at day 14. Denervated PLD and ALD muscles were stimulated from day 4 to day 14 of age. Two patterns of stimulation were applied, either 5-Hz frequency (slow rhythm) or 40-Hz frequency (fast rhythm). Both patterns of stimulation provided the same number of impulses per day (about 61,000). In PLD muscle, electrical stimulation almost totally prevented the postdenervation loss in asymmetric forms and led to a decrease in BuChE specific activity. In ALD muscle, electrical stimulation partially prevented the asymmetric form loss which occurs after denervation. This study emphasizes the role of evoked muscle activity in the regulation of BuChE asymmetric forms in the fast PLD muscle and the differential response of denervated slow and fast muscles to electrical stimulation.

show abstract

Glucocorticoid increases acetylcholinesterase and organization of the postsynaptic membrane in innervated cultured human muscle

Askanas¹,

McFerrin²,

Park-Matsumoto³

et al. 1992

Experimental Neurology

View full text Add to dashboard Cite

Posthatching changes in levels and molecular forms of acetylcholinesterase in slow and fast muscles of the chicken: Effects of denervation and direct electrical stimulation

Cited by 6 publications

References 38 publications

Developmental modulation of physicochemical variants of the tailed asymmetric (16S) acetylcholinesterase by neuromuscular activity and innervation in the mouse embryo

Developmental modulation of physicochemical variants of the tailed asymmetric (16S) acetylcholinesterase by neuromuscular activity and innervation in the mouse embryo

Effects of Electrical Stimulation on Molecular Forms of Butyrylcholinesterase in Denervated Fast and Slow Latissimus Dorsi Muscles of Newly Hatched Chicken

Glucocorticoid increases acetylcholinesterase and organization of the postsynaptic membrane in innervated cultured human muscle

Contact Info

Product

Resources

About