Effects of date seeds administration on anxiety and depressive symptoms in streptozotocin-induced diabetic rats: biochemical and behavioral evidences

Abstract

Objectives Several epidemiological data indicate that chronic hyperglycemia is associated with behavioral changes such as anxiety and depressive symptoms. Date seeds, one of the most potent products with potential antioxidant activities and possess many benefits against hyperglycemia and its complication. The aim of the current study was to explore the potential effect of date seeds extract on biochemical and behavioral changes (anxiety and depression) in streptozotocin (STZ)-diabetic rats. Methods Rats were divided into four groups as follows: normal control, diabetic control, diabetic treated with the lyophilized aqueous extract of the date seed (2,000 mg/kg) (LAE-DS) and diabetics treated with insulin (4 UI/day). Experimental diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg). After 24 days treatment period, anxiety and depressive behaviors were evaluated using four behavioral tests. After sacrifice, blood samples were collected to evaluate lipid parameters. In addition, rat organs (kidney, liver and brain) were dissected out in order to estimate lipid peroxidation levels as oxidative stress marker. Results Oral administration of the lyophilized aqueous extract of date seeds and insulin injection for 30 days significantly decreased blood glucose levels in STZ-diabetic rats and protected them against undesirable changes in lipid parameters, including cholesterol, triglycerides, LDL cholesterol, VLDL cholesterol and atherosclerosis index. Compared to untreated diabetic rat, a significant decrease in lipid peroxidation levels in kidney, liver and brain (Hippocampus and prefrontal cortex) were observed after treatment with insulin or LAE-DS in diabetic rats. Furthermore, insulin and LAE-DS administration prevented anxiety-related behaviors in STZ-diabetic rats. Conclusions Therefore, it would be possible to combine this extract with insulin and use it as an antioxidant supplement for type 1 diabetic patients.

LC/ESI/TOF-MS Characterization, Anxiolytic and Antidepressant-like Effects of Mitragyna speciosa Korth Extract in Diabetic Rats

“…The results from this study revealed that DM led to characteristic anxiety and depressive-like behaviors, as indicated by the increase in immobility duration (FST), decrease in entries and time spent in the open arms (EPMT) as well as reduction in the number of crossing and rearing (OFT), which were in corroboration with previous studies on DM-induced anxiety and depression [ 8 , 26 , 31 , 32 ]. Metabolic disorders, including diabetes and obesity, have been prominently associated a number of psychological problems particularly major depressive disorders and anxiety, and hyperglycemia is a prevalent factor that can modulate the onset, progression and deterioration of depression and anxiety [ 33 ].…”

Insulin supplemented with phenolic fraction concentrates displays anxiolytic and antidepressant-like properties with reductions of oxidative brain damage in chronically stressed diabetic rats

“…Insulin supplemented with PFC had more significant effects on anxiety than only insulin treatment in STZ-diabetic rats exposed to CMS, as revealed by the decreased symptoms assessed using the OFT and EPM tests. This anxiolytic-like effect of insulin has previously been reported in STZ-diabetic rats (53). The current study is the first report that shows this treatment combination has these effects on STZ rat model of diabetes under stress conditions.…”

“…One reason for these findings could be explained by the experimental procedures and the total duration of stress exposition. In this line, antidepressant-like effects of insulin were previously reported in STZ-diabetics rats (21,53,64). Contrariwise, our results revealed that stressed diabetic rats treated with insulin were less likely to decrease the immobility time in FST, indicating the absence of any significant antidepressant effect of insulin in this model of rats.…”

Innate immune stimulation by monophosphoryl lipid A prevents chronic social defeat stress-induced anxiety-like behaviors in mice

“…In daily life, the anxiety-associated behaviors can be induced by many factors, such as repeated social defeat stress [ 6 , 30 ], high-fat intake [ 31 ], and metabolic disturbance [ 32 ]. Rocha-Gomes et al reported that a low dose of LPS injection during mouse gestation can induce a preventive effect on anxiety-like behaviors and neuroinflammatory responses in the amygdala in high-fat-fed dam’s adolescent offspring [ 33 ].…”