Effects of Danhong Injection on platelet aggregation in hyperlipidemia rats

Fan, Haojun; Li, Min; Yu, Li; Jin, Weifeng; Yang, Jiehong; Zhang, Yuyan; Wan, Haitong

doi:10.1016/j.jep.2017.10.017

Cited by 37 publications

(27 citation statements)

References 40 publications

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“…Several reports demonstrated that the levels of plasma TC, TG, HDL-C, and LDL-C, which have significance from normal rats, can suggest the establishment of hyperlipidemia [21,[27][28][29]. The experimental results indicated that the plasma CL increased after the combined medication of PAL and HSYA in the hyperlipidemia model and the difference was significant (p < 0.01), which suggests that the 2 drugs influence each other greatly in plasma CL.…”

Section: Discussionmentioning

confidence: 86%

“…It was found that there was some mutual influence of combined medication in vivo pharmacokinetic. However, the vivo efficacy of compound drugs cannot be identified and described only in terms of pharmacokinetics [27]. And further study will be carried out to explore the difference on pharmacodynamics of single and combined medication of PAL and HSYA and establish a variety of pharmacokinetic-pharmacodynamic analysis methods to guide the clinical application of drugs better and rationally in this field.…”

Section: Discussionmentioning

confidence: 99%

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Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Zhou¹,

Li²,

Jin³

et al. 2018

Pharmacology

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Background: Protocatechuic aldehyde (PAL) and hydroxysafflor yellow A (HSYA) are 2 effective ingredients of Danhong Injection, which is extensively used for the clinical treatment of cardio-cerebrovascular diseases. This study aims to investigate the pharmacokinetic differences between single and combined medication of PAL and HSYA and analyze the interaction of the above effective components in hyperlipidemia rats. Methods: Thirty male SD rats were randomly divided into the control group (n = 6) and the model group (n = 24). The hyperlipidemia model was established by feeding with superfatted forage. The successful model rats were then randomly divided into the PAL group (16 mg/kg), the HSYA group (10 mg/kg), and the combination group (16 mg/kg + 10 mg/kg). Administration through tail-vein, and orbital blood was sampled at different time points. The mass concentration of PAL and HSYA was determined by high performance liquid chromatography (HPLC-DAD). Analysis of pharmacokinetic parameters was conducted by using DAS 3.2.6 software and SPSS 19.0 statistical analysis software. Results: According to the parameters of statistical moment of non-compartmental model, there was a significant difference in plasma clearance (CL) between the PAL group and the drug combination group (p < 0.01), as well as in the area under the first moment of the plasma concentration-time curve and the elimination half-life (t_1/2) between the HSYA group and the drug combination group (p < 0.01) but no obvious differences about the blood concentration time curve area, the average dwell time (MRT), and the peak concentration (C_max; p > 0.05). Conclusion: The combined medication of PAL and HSYA could increase the plasma CL significantly and have a great influence on the absorption of HSYA in rats with hyperlipidemia.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Zhou¹,

Li²,

Jin³

et al. 2018

Pharmacology

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“…TXA 2 induces vasoconstriction and platelet aggregation and promotes AS formation [35], whereas PGI 2 has the function of relaxing blood vessels and inhibiting platelet aggregation [36]. e balance between TXA 2 and PGI 2 maintains the basic function of blood vessels and platelets, ensuring the stable blood circulation, which plays a vital role in the prevention and treatment of AS [37]. Since TXA 2 and PGI 2 are both unstable in vivo, it is difficult to measure them directly.…”

Section: Discussionmentioning

confidence: 99%

Antiatherosclerosis Properties of Total Saponins of Garlic in Rats

Miao

Wang

Bai

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Garlic has been proven effective in the prevention and treatment of atherosclerosis (AS), which is widely used as a food and medicine by people in daily life. Garlic saponins are the main active nonsulfur compounds of garlic, which have a variety of pharmacological activities against cardiovascular diseases. In this study, the antiatherosclerosis properties and mechanism of total saponins of garlic (TSG) in rats were explored. The AS animal model was established by a combination of high-fat feeding, intraperitoneal injection of vitamin D3, and ovalbumin-induced inflammation in SD rats. Then, the atherosclerotic rats were gavaged daily by TSG for 4 weeks. Administration of TSG markedly decreased atherosclerotic lesions in the aorta of atherosclerotic rats. TSG restored the serum lipid profile by significantly decreasing the lipid levels and had effective antioxidation by inhibiting the content of malondialdehyde (MDA) and restoring the reduced activity of superoxide dismutase (SOD). Additionally, the ratio of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) could be maintained in a relatively stable dynamic balance after administration of TSG to maintain the vascular homeostasis. In summary, TSG had therapeutic effects on AS, which are promising as functional foods or nutraceuticals for the prevention and treatment of AS.

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“…Existing studies have shown that DSS, SAA, SAB, and HSYA have the ability to scavenge free oxygen radicals, inhibit apoptosis, and prevent inflammation [5][6][7][8][9][10][11][12][13][14][15][16][17][18][25][26][27][28], thereby protecting against cerebral ischemic injury. Over the past few years, the Dan-Hong injection and its ingredients have been widely studied in clinical and basic research [30,[46][47][48]. However, there are few studies evaluating the pharmacokinetic parameters of the active ingredients in the Dan-Hong injection.…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetics of Active Ingredients of Salvia miltiorrhiza and Carthamus tinctorius in Compatibility in Normal and Cerebral Ischemia Rats: A Comparative Study

Ying

et al. 2019

Eur J Drug Metab Pharmacokinet

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Background and Objective Dan-Hong injection, which comprises extracts of Salvia miltiorrhiza and Carthamus tinctorius, promotes blood circulation and reduces blood stasis. Combination of S. miltiorrhiza and C. tinctorius is more effective in treating cerebral ischemia than S. miltiorrhiza alone. This study aimed to examine the pharmacokinetic characteristics of four active ingredients of S. miltiorrhiza and C. tinctorius, namely danshensu (DSS), hydroxysafflor yellow A (HSYA), and salvianolic acid A (SAA) and B (SAB) in normal and cerebral ischemia rats. Methods Normal and cerebral ischemia rats were injected via the tail vein with each active ingredient, and blood was collected through the jaw vein at different time points. The plasma concentration of the compatibility group was analyzed by high-performance liquid chromatography, and pharmacokinetic parameters were determined using Pharmacokinetic Kinetica 4.4 software. Results The pharmacokinetics of the four active ingredients in the normal and cerebral ischemia rats were consistent with a two-compartment model. The area under the concentration-time curve was higher in normal rats than in cerebral ischemia rats, with a highly significant difference for SAA (P < 0.01). Clearance rates were lower in normal rats than in cerebral ischemia rats, with DSS showing the most significant difference (P < 0.01). Furthermore, there were significant differences between normal and cerebral ischemia rats in the distribution phase-elimination half life for DSS, SAA, and HSYA, as well as in the apparent volume of distribution for the central compartment for DSS and HSYA (P < 0.01). The plasma concentrations of the four active ingredients were higher in normal rats than in cerebral ischemia rats. Conclusion Cerebral ischemia rats showed higher drug clearance rates and longer retention times than normal rats, which may be due to destruction of the blood-brain barrier during cerebral ischemia-reperfusion. The four active ingredients likely integrated and interacted with each other to affect target sites in the brain to protect against cerebral ischemic injury.

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Effects of Danhong Injection on platelet aggregation in hyperlipidemia rats

Cited by 37 publications

References 40 publications

Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Antiatherosclerosis Properties of Total Saponins of Garlic in Rats

Pharmacokinetics of Active Ingredients of Salvia miltiorrhiza and Carthamus tinctorius in Compatibility in Normal and Cerebral Ischemia Rats: A Comparative Study

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