Effects of cytochalasin H on chick embryo explants cultured in vitro

Actin bundles on the right side in the caudal part of the heart tube play a role in dextro-looping in the embryonic chick heart

“…They bind to the barbed end of actin filaments (F-actin) and inhibit association and dissociation of subunits at that end (Cooper 1987), and subsequently cause disorganization of the actin bundles. When embryos were cultured with cytochalasin dissolved in the medium, the heart remained a symmetrical structure, though it continued to increase in size (Manasek 1976, Ghaskadbi andMulherkar 1984). This result indicates that actin bundles are involved in asymmetrical loop formation.…”

Actin bundles on the right side in the caudal part of the heart tube play a role in dextro-looping in the embryonic chick heart

“…They bind to the barbed end of actin filaments (F-actin) and inhibit association and dissociation of subunits at that end (Cooper 1987), and subsequently cause disorganization of the actin bundles. When embryos were cultured with cytochalasin dissolved in the medium, the heart remained a symmetrical structure, though it continued to increase in size (Manasek 1976, Ghaskadbi andMulherkar 1984). This result indicates that actin bundles are involved in asymmetrical loop formation.…”

β‐Microseminoprotein‐related molecules may participate in formation of the mesoderm in the chick embryo

“…Gastrulating chick embryos of Hamburger–Hamilton (HH) stages 3, 4, 4+ and 5 were used in the present study. Embryos were treated as described previously (Ghaskadbi & Mulherkar 1984; Patwardhan et al . 1996).…”

Quantitative Analysis of 3D Tissue Deformation Reveals Key Cellular Mechanism Associated with Initial Heart Looping

“…Rearrangement that Causes Longitudinal Deformation of Right Tissue Cell rearrangement is widely observed during organ/animal developmental processes, and acto-myosin activity is often involved (Guillot and Lecuit, 2013). During early heart development, inhibition of actin polymerization abolishes looping (Manasek et al, 1972a;Ghaskadbi and Mulherkar, 1984;Itasaki et al, 1991;Latacha et al, 2005). However, the role of myosin function in looping remains controversial because several studies reported a role for myosin II phosphorylation in C-looping (Lu et al, 2008;Noë l et al, 2013;Ocañ a et al, 2017), whereas others saw no effect (Latacha et al, 2005;Nerurkar et al, 2006;Shi et al, 2014b).…”

Actin bundles on the right side in the caudal part of the heart tube play a role in dextro-looping in the embryonic chick heart

“…They bind to the barbed end of actin filaments (F-actin) and inhibit association and dissociation of subunits at that end (Cooper 1987), and subsequently cause disorganization of the actin bundles. When embryos were cultured with cytochalasin dissolved in the medium, the heart remained a symmetrical structure, though it continued to increase in size (Manasek 1976, Ghaskadbi andMulherkar 1984). This result indicates that actin bundles are involved in asymmetrical loop formation.…”

β‐Microseminoprotein‐related molecules may participate in formation of the mesoderm in the chick embryo

“…Gastrulating chick embryos of Hamburger–Hamilton (HH) stages 3, 4, 4+ and 5 were used in the present study. Embryos were treated as described previously (Ghaskadbi & Mulherkar 1984; Patwardhan et al . 1996).…”

Quantitative Analysis of 3D Tissue Deformation Reveals Key Cellular Mechanism Associated with Initial Heart Looping

“…Rearrangement that Causes Longitudinal Deformation of Right Tissue Cell rearrangement is widely observed during organ/animal developmental processes, and acto-myosin activity is often involved (Guillot and Lecuit, 2013). During early heart development, inhibition of actin polymerization abolishes looping (Manasek et al, 1972a;Ghaskadbi and Mulherkar, 1984;Itasaki et al, 1991;Latacha et al, 2005). However, the role of myosin function in looping remains controversial because several studies reported a role for myosin II phosphorylation in C-looping (Lu et al, 2008;Noë l et al, 2013;Ocañ a et al, 2017), whereas others saw no effect (Latacha et al, 2005;Nerurkar et al, 2006;Shi et al, 2014b).…”

Actin bundles on the right side in the caudal part of the heart tube play a role in dextro-looping in the embryonic chick heart

“…They bind to the barbed end of actin filaments (F-actin) and inhibit association and dissociation of subunits at that end (Cooper 1987), and subsequently cause disorganization of the actin bundles. When embryos were cultured with cytochalasin dissolved in the medium, the heart remained a symmetrical structure, though it continued to increase in size (Manasek 1976, Ghaskadbi andMulherkar 1984). This result indicates that actin bundles are involved in asymmetrical loop formation.…”

β‐Microseminoprotein‐related molecules may participate in formation of the mesoderm in the chick embryo

“…Gastrulating chick embryos of Hamburger–Hamilton (HH) stages 3, 4, 4+ and 5 were used in the present study. Embryos were treated as described previously (Ghaskadbi & Mulherkar 1984; Patwardhan et al . 1996).…”

Quantitative Analysis of 3D Tissue Deformation Reveals Key Cellular Mechanism Associated with Initial Heart Looping

“…Rearrangement that Causes Longitudinal Deformation of Right Tissue Cell rearrangement is widely observed during organ/animal developmental processes, and acto-myosin activity is often involved (Guillot and Lecuit, 2013). During early heart development, inhibition of actin polymerization abolishes looping (Manasek et al, 1972a;Ghaskadbi and Mulherkar, 1984;Itasaki et al, 1991;Latacha et al, 2005). However, the role of myosin function in looping remains controversial because several studies reported a role for myosin II phosphorylation in C-looping (Lu et al, 2008;Noë l et al, 2013;Ocañ a et al, 2017), whereas others saw no effect (Latacha et al, 2005;Nerurkar et al, 2006;Shi et al, 2014b).…”