Effects of cytochalasin H on chick embryo explants cultured in vitro

Ghaskadbi, Surendra; Mulherkar, Leela

doi:10.1016/0300-483x(84)90048-9

Cited by 7 publications

(4 citation statements)

References 19 publications

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“…They bind to the barbed end of actin filaments (F-actin) and inhibit association and dissociation of subunits at that end (Cooper 1987), and subsequently cause disorganization of the actin bundles. When embryos were cultured with cytochalasin dissolved in the medium, the heart remained a symmetrical structure, though it continued to increase in size (Manasek 1976, Ghaskadbi andMulherkar 1984). This result indicates that actin bundles are involved in asymmetrical loop formation.…”

Section: Introductionmentioning

confidence: 80%

Actin bundles on the right side in the caudal part of the heart tube play a role in dextro-looping in the embryonic chick heart

et al. 1991

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The role of actin bundles on the heart looping of chick embryos was examined by using cytochalasin B, which binds to the barbed end of actin filaments and inhibits association of the subunits. It was applied to embryos cultured according to New's method. Looping did not occur when cytochalasin B was applied diffusely in the medium. Further, we disorganized actin bundles in a limited part of the heart tube to examine the role of actin bundles in each part in asymmetry formation. A small crystal of cytochalasin B was applied to the caudal part of the heart tube on either the left or right side. The disorganization of actin bundles on the left side resulted in the right-bending of the heart, an initial sign of dextro-looping (normal pattern), and right side disorganization resulted in left-bending. We suggest that actin bundles on the right side of the caudal part of a heart tube generate tension and cause dextro-looping. Embryos whose hearts bent to the right rotated their heads to the right, and embryos with left-bent-hearts rotated their heads to the left. The rotation of the heart tube may therefore decide in which direction the body axis rotates.

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Section: Introductionmentioning

confidence: 80%

Actin bundles on the right side in the caudal part of the heart tube play a role in dextro-looping in the embryonic chick heart

et al. 1991

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“…Rearrangement that Causes Longitudinal Deformation of Right Tissue Cell rearrangement is widely observed during organ/animal developmental processes, and acto-myosin activity is often involved (Guillot and Lecuit, 2013). During early heart development, inhibition of actin polymerization abolishes looping (Manasek et al, 1972a;Ghaskadbi and Mulherkar, 1984;Itasaki et al, 1991;Latacha et al, 2005). However, the role of myosin function in looping remains controversial because several studies reported a role for myosin II phosphorylation in C-looping (Lu et al, 2008;Noë l et al, 2013;Ocañ a et al, 2017), whereas others saw no effect (Latacha et al, 2005;Nerurkar et al, 2006;Shi et al, 2014b).…”

Section: Actin Polymerization Is Required For Directional Cellmentioning

confidence: 99%

Quantitative Analysis of 3D Tissue Deformation Reveals Key Cellular Mechanism Associated with Initial Heart Looping

et al. 2020

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“…Gastrulating chick embryos of Hamburger–Hamilton (HH) stages 3, 4, 4+ and 5 were used in the present study. Embryos were treated as described previously (Ghaskadbi & Mulherkar 1984; Patwardhan et al . 1996).…”

Section: Methodsmentioning

confidence: 99%

β‐Microseminoprotein‐related molecules may participate in formation of the mesoderm in the chick embryo

Karandikar

Ghaskadbi

2003

Dev Growth Differ

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It has previously been shown that human ␤ -microseminoprotein enhances development of mesodermal structures in the chick embryo. The present study was carried out to elucidate the mechanism of action of human ␤ -microseminoprotein in the chick embryo. ␤ -Microseminoprotein brought about significant modulation of expression of Brachyury in gastrulating embryos. In approximately 50% of the treated embryos, Brachyury expression was enhanced around the Hensen's node. These cells not only expressed higher levels of Brachyury , but also appeared to switch off Brachyury expression prematurely, postinvagination. The spatial modulation of Brachyury is not clearly reflected in the northern blots, indicating that ␤ -microseminoprotein treatment results in redistribution of available transcripts or that the upregulation is compensated for by early switching off of Brachyury postinvagination. Because higher levels of Brachyury during gastrulation are believed to result in early exit of cells from the primitive streak, ␤ -microseminoprotein treatment appeared to have stimulated morphogenetic movements by upregulating Brachyury around the Hensen's node. This deduction was confirmed by scanning electron microscopic analysis that showed that altered morphogenetic movements accompany modulation of Brachyury . The specific responses elicited by ␤ -microseminoprotein indicate presence of a structurally related molecule in the chick. By western blotting, similar molecules were indeed detected in the chicken seminal plasma and in chick embryos. These data strongly suggest that ␤ -microseminoprotein-related molecule(s) participates in mesoderm formation in the chick embryo.

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Effects of cytochalasin H on chick embryo explants cultured in vitro

Cited by 7 publications

References 19 publications

Actin bundles on the right side in the caudal part of the heart tube play a role in dextro-looping in the embryonic chick heart

Actin bundles on the right side in the caudal part of the heart tube play a role in dextro-looping in the embryonic chick heart

Quantitative Analysis of 3D Tissue Deformation Reveals Key Cellular Mechanism Associated with Initial Heart Looping

β‐Microseminoprotein‐related molecules may participate in formation of the mesoderm in the chick embryo

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