Effects of cytochalasin E on<i>Paracoccidioides brasiliensis</i>

Mendes, Graziele; Baltazar, Ludmila Matos; Souza, Daniele G.; Sá, Nívea Pereira de; Rosa, Luiz Henrique; Rosa, Carlos A.; Souza-Fagundes, Elaine M.; Ramos, Jonas Pereira; Silva, Juliana Alves da; Cota, Betânia Barros; Johann, Susana

doi:10.1111/jam.14053

Cited by 3 publications

(3 citation statements)

References 41 publications

(42 reference statements)

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“…Nine compounds were present in the pure culture of C. coccodes growing on MEA plates. They were mainly unspecific components with the exception of bacterial monocerin and cytochalasin E, originally isolated from the Aspergillus species [ 65 ], as a probable effect of cross-contamination. Eleven metabolites were identified in potatoes inoculated with C. coccodes , mostly unrelated to fungal activity, except for several compounds originally isolated from the Fusarium species, such as beauvericin, bikaverin, and deoxygerfelin ( Table 5 ).…”

Section: Resultsmentioning

confidence: 99%

Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents

Steglińska

Sulyok

Janas

et al. 2023

IJERPH

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The potato is a crop of global importance for the food industry. This is why effective protection against pathogens is so important. Fungi as potato pathogens are responsible for plant diseases and a significant reduction in yields, as well as for the formation of mycotoxins. This study focuses on the effect of three natural biocides, yeast Metschnikowia pulcherrima, lactic acid bacteria Lactiplantibacillus plantarum, and aqueous garlic extract, on the improvement of the physiology of planted potato tubers and the reduction in mycotoxin formation. The secondary metabolites produced by the fungal pathogens of genera Fusarium, Alternaria, Colletotrichum, Rhizoctonia, and Phoma in the presence of these biocontrol agents were compared to profiles obtained from contaminated potatoes. Analysis of liquid chromatography coupled with tandem mass spectrometry data showed the presence of 68 secondary metabolites, including the mycotoxins: alternariol, alternariol methyl ether, altertoxin-I, aurofusarin, beauvericin, diacetoxyscirpenol, enniatin B, and sterigmatocystin. The studies showed that the applied biocontrol agents had a positive effect on the physiological parameters of potatoes (including root growth, stem growth, gas exchange, and chlorophyll content index) and on the reduction in the production of mycotoxins and other secondary metabolites by Fusarium, Alternaria, and Phoma.

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Section: Resultsmentioning

confidence: 99%

Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents

Steglińska

Sulyok

Janas

et al. 2023

IJERPH

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“…(Table S2). The extracts and cytochalasin E isolated from Aspergillus felis presented an MIC of 31.2 µg/mL (3.6 µM) [55]. The extract of the endophytic fungus Fusarium sp.…”

Section: New Anti-paracoccidioides Compounds: Do We Already Have Any Ideal Antifungals?mentioning

confidence: 99%

Overview of Antifungal Drugs against Paracoccidioidomycosis: How Do We Start, Where Are We, and Where Are We Going?

Silva

Oliveira

Souza

et al. 2020

JoF

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Paracoccidioidomycosis is a neglected disease that causes economic and social impacts, mainly affecting people of certain social segments, such as rural workers. The limitations of antifungals, such as toxicity, drug interactions, restricted routes of administration, and the reduced bioavailability in target tissues, have become evident in clinical settings. These factors, added to the fact that Paracoccidioidomycosis (PCM) therapy is a long process, lasting from months to years, emphasize the need for the research and development of new molecules. Researchers have concentrated efforts on the identification of new compounds using numerous tools and targeting important proteins from Paracoccidioides, with the emphasis on enzymatic pathways absent in humans. This review aims to discuss the aspects related to the identification of compounds, methodologies, and perspectives when proposing new antifungal agents against PCM.

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“…The choice of the drug to be administered depends on various circumstances such as clinical severity, previous history of therapeutic resistance, gastrointestinal drug absorption capacity, association with comorbidities, and treatment adherence. P. brasiliensis is sensitive to most antifungal drugs, including sulfonamides, azoles (ketoconazole, fluconazole, itraconazole, posaconazole, voriconazole), amphotericin B (AB), and terbinafine (Mahajan, 2014;Ambrósio et al, 2014;Orofino-Costa et al, 2017) Sulfamethoxazole-trimethoprim or itraconazole can be used in the mild or moderate localized forms (Borges et al, Research, Society and Development, v. 11, n. 12, e165111234460, 2022 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v11i12.34460 3 2014; Mendes et al, 2018) being amphotericin B reserved for the disseminated and severe forms, as in the central nervous system (CNS) (Hahn & Hamdan, 2000;Alonso et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Development of gelatin nanoparticles for the treatment of pathologies caused by Paraccocideoides brasiliensis

Silva

Gouvea²,

Ambrósio³

et al. 2022

RSD

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Introduction: Paraccocideoides brasiliensis (Pb) is a fungal pathology that causes serious lesions in the mouth in the form of ulcerations that destroy the gingiva, palate, reflecting on the immune system. For the treatment of Paracoccidioidomycosis (PMC) the choice of drug is based on the degree of the disease, with Amphotericin B (AB) being the most used due to its mechanism of action and interaction with the fungal membrane, causing its destruction. However, with all the history of AB toxicity, there is a need to reduce the serum levels of the drug in the body. The use of nanoparticles as controlled release systems (DDS) is an alternative for this purpose. The objective of this article was to develop gelatin nanoparticles for the treatment of pathologies caused by Pb. Method: In this work, gelatin nanoparticles were processed by the two-step desolvation method and loaded with AB. The nanoparticulate system was characterized by scanning electron microscopy (SEM) and dynamic light scattering (DLS). Finally, the effectiveness of DDS was evaluated against Pb yeasts by Disk Diffusion Test. Results: the synthesized particles presented suitable sizes and shapes for application in DDS with an average size of 169.4 and 172.0 nm, for the free and encapsulated system, respectively. Biological model tests of Pb showed positive results indicating that lower concentrations of AB can be efficiently administered if nanostructured DDS is used. Conclusion: This study is promising for future applications of AB-loaded gelatin nanoparticles for the treatment of pathologies caused by Paraccocideoides brasiliensis.

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Effects of cytochalasin E onParacoccidioides brasiliensis

Cited by 3 publications

References 41 publications

Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents

Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents

Overview of Antifungal Drugs against Paracoccidioidomycosis: How Do We Start, Where Are We, and Where Are We Going?

Development of gelatin nanoparticles for the treatment of pathologies caused by Paraccocideoides brasiliensis

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