Effects of CYP1A2 Gene Polymorphisms on Antipyrine CYP1A2-Dependent Metabolism

Chernyak, Yu. I.; Ицкович, В. Б.; Kolesnikov, S. I.

doi:10.1007/s10517-011-1352-6

Cited by 3 publications

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“…AZF-локус -не единственная детерминанта спермато-генеза. Стерильность и блок сперматогенеза могут стать следствием изменений в гене CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) и привести к тяжелому наследственному заболеванию -муковисцидозу [19]. В результате мутаций в гене CFTR у мужчин диагностиру-ется обструктивная азооспермия, в 25% случаев являю-щаяся следствием одно-или двустороннего врожденного отсутствия семявыносящих протоков.…”

Section: генетические маркеры мужской инфертильностиunclassified

“…Под влиянием фрагментированных ДНК особенно стра-дают ранние этапы эмбрионального развития (форми-рование бластоцисты). Также от наличия поврежденных цепочек ДНК зависят частота наступления беременности и положительный репродуктивный исход [19,20].…”

Section: генетические маркеры мужской инфертильностиunclassified

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Causes and Factors of Male Infertility

et al. 2015

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В настоящее время проблемы здоровья населения стра-ны, рождаемости и перспективы демографии -ключевые не только в России, но и за рубежом. Неблагоприятные демографические показатели с устойчивым отрицательным коэффициентом естественного прироста населения в по-следние десятилетия заставляют специалистов различного профиля (генетиков, морфологов, иммунологов, эндокри-нологов, гинекологов, урологов) обратиться к анализу фак-торов, влияющих на рождаемость, среди которых важное место занимает бесплодие [1][2][3]. Согласно статистическим данным, частота бесплодных браков во многих странах мира колеблется от 8 до 29%. В Европе бесплодием страдает око-ло 10% супружеских пар, в США -15%, в Канаде -17%, доля бесплодных браков в России варьирует от 8,2 до 19,6%. Приведенные показатели превышают критический уровень (15%) и представляют государственную проблему, имея много составляющих (социально-демографическую, ме-дицинскую, экономическую и др.) [1][2][3]

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Section: генетические маркеры мужской инфертильностиunclassified

Causes and Factors of Male Infertility

et al. 2015

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“…Focusing on CYP1A2, a hepatic cytochrome enzyme, which is responsible for the metabolism of a number of substrates in addition to caffeine that are important in the human body. These substrates include procarcinogens, hormones, drugs, endogenous compounds, as well as enzyme activity in tobacco smokers (Chernyak et al 2011). Procarcinogens like Benzo[a]pyrene, which is a harmless chemical found in many foods like grilled meats and tobacco smoke (Zhou et al 2009).…”

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Analysis of the CYP1A2 caffeine metabolism gene in the student population at Lake Superior State University

Pittsley

Kolomyjec

2022

Preprint

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85% of Americans drink caffeinated beverages on a daily basis. Each individual responds differently to caffeine depending on age, gender, diet, and ethnicity. Caffeinated beverages cause insomnia in some people, but not in others due to differences in the rate of caffeine metabolism. This study examines the variation in the caffeine metabolism of Lake Superior State University (LSSU) students. My hypothesis was that LSSU student allele frequencies would match those of the general population: 47.5% fast, 41.0% medium, and 11.5% slow caffeine metabolism. 200 LSSU students were sampled via buccal swabs. DNA was successfully isolated from 164 samples. Participants filled out a demographic questionnaire entailing caffeine intake, ethnicity, and sex. The CYP1A2 gene was amplified via standard PCR prior to genotyping by restriction digest and gel electrophoresis. The APAI restriction enzyme was used to determine the genotype of the rs762551 single nucleotide polymorphism (SNP), while the SACI enzyme was used as a positive digestion control. Overall, results showed a total of 42.7% fast, 44.5% medium, and 12.8% slow metabolizers. Of special note is that 24 of the 164 students sampled were of Native American heritage, an important yet underrepresented group in human genomics. This study provides the first reported look at the CYP1A2 variation within this North American subpopulation with metabolism rates being 50% fast, 33.3% medium, and 16.7% slow. The results confirm my initial hypothesis that the variation of caffeine metabolism gene frequencies for the LSSU student population would be representative of published allele frequencies for the general population.

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Role of Cytochrome P450-Dependent Monooxygenases and Polymorphic Variants of GSTT1 and GSTM1 Genes in the Formation of Brain Lesions in Individuals Chronically Exposed to Mercury

et al. 2013

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The metabolic test with antipyrine was performed, the relationship between genotypes of GSTT1 and GSTM1 polymorphisms were studied, and cotinine level was measured in 116 men chronically exposed to mercury. The individuals were divided in 4 groups depending on the diagnosis of chronic mercury intoxication. The changes in the parameters of antipyrine test were studied in linked samples (N=62, 4 year interval); in patients with chronic mercury intoxication, the disease stage was taken into account. Inhibition of antipyrine metabolism, increased frequency of combination of GSTT1(0/0)/GSTM1(+) genotypes in patients with chronic mercury intoxication, and the specificity of cytochrome P450 inhibition with mercury suggest that disease progression is related to inhibition of cytochrome P450 isoforms in the brain that catalyze regulation of endogenous substrates.

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Effects of CYP1A2 Gene Polymorphisms on Antipyrine CYP1A2-Dependent Metabolism

Cited by 3 publications

References 12 publications

Causes and Factors of Male Infertility

Causes and Factors of Male Infertility

Analysis of the CYP1A2 caffeine metabolism gene in the student population at Lake Superior State University

Role of Cytochrome P450-Dependent Monooxygenases and Polymorphic Variants of GSTT1 and GSTM1 Genes in the Formation of Brain Lesions in Individuals Chronically Exposed to Mercury

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