Effects of Cyclophosphamide on Open, Granulating Skin Wounds in Rats

Wie, Henrik; Bruaset, I.; Eckersberg, Thomas

doi:10.1111/j.1699-0463.1979.tb00041.x

Cited by 5 publications

(6 citation statements)

References 11 publications

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“…The present investigation reveals marked differences between the effects of low doses of cyclophosphamide, giving about 40% reduction of the WBC count and high doses giving more than 70% reduction of the WBC count (Wie et al 1979b).…”

Section: Discussionmentioning

confidence: 48%

“…At high dose levels of cyclophosphamide the effects on collagen synthesis seem to be the main cause of the reduced mechanical properties which were reported in a previous paper (Wie et al 1979b). At low dose levels, however, the effects on collagen stability may dominate.…”

Section: Discussionmentioning

confidence: 55%

“…Corresponding cyclophosphamide regimens have been shown to produce dose dependent increased fragility of growing bones and wounded skin in young rats (Wie et a/. 1979b).…”

Section: Discussionmentioning

confidence: 99%

“…After intramuscular anaesthesia (HypnormB, Mekos) the backs of the animals were shaved and cleansed with 0.5% chlorhexidine. Incisional skin wounds were inflicted under aseptic conditions using the procedure described by Wie et at. (1979b), and porous ceramic implants were inserted subcutaneously on the left side of the animals (Wie & Beck 1979).…”

Section: Methodsmentioning

confidence: 99%

“…1979a) Observations on the effects of this agent o n incisional surgical wounds are inconsistent, probably due t o the wide range of dose levels and dose schedules which have been used (Cohen et al 1975). Intact a n d wounded skin and femoral bones of growing rats and mice have been found to be affected by the drug in doses comparable t o therapeutic doses in man (Freireich et al 1966;Cohen et al 1975;Wie et al 1979b).…”

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confidence: 99%

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Effects of Cyclophosphamide on the Formation and Solubility of Collagen

Wie

Beck

1981

Acta Pharmacologica et Toxicologica

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The effects of low (5 mg/kg × 7) and high (20 or 30 mg/kg × 7) doses of cyclophosphamide on the formation an solubility of collagen in subcutaneous, porous implants, bones and incisional skin wounds were studied in young, male rats. At the 5 mg/kg schedule, effects from the drug were only detected as an increased solubility of collagen in implant connective tissue. At the 20 mg/kg schedule, there was a significant reduction of the synthesis and solubility of collagen in bones and in skin wounds. The 30 mg/kg schedule significantly depressed all the parameters studied except the specific activity of hydroxyproline in implants. Collagen stability seems to be impaired at low dose levels, whereas one of the main effects of high doses appears to be inhibition of collagen synthesis.

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 55%

“…Corresponding cyclophosphamide regimens have been shown to produce dose dependent increased fragility of growing bones and wounded skin in young rats (Wie et a/. 1979b).…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of Cyclophosphamide on the Formation and Solubility of Collagen

Wie

Beck

1981

Acta Pharmacologica et Toxicologica

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Mechanical properties of bone and skin during recovery after cyclophosphamide administration

Wie

Beck

Engesæter

et al. 1983

Arch. Orth. Traum. Surg.

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The effects of cyclophosphamide were studied 2, 5 and 9 days after terminating a two-week every-second-day intraperitoneal medication in young, male rats. The cytostatic effect was assessed by counting white blood cells (WBC) in arterial blood. WBC counts were reduced by 72% of control values at 2 days and regained normal values at 9 days after an overshooting leucocytosis at 5 days. Compared to control animals, the treated rats had significantly reduced body weights, metaphyseal bending strength and longitudinal growth of the femur at all time intervals observed. The torsional strength of the femur diaphysis and the strength of wounded skin were not affected at 2 days, but significantly reduced at 5 and 9 days. The tensile strength of intact skin was not found to be affected by the drug. From the 5th to the 9th day after ending medication, the curves for control and treated animals were assuming parallel slopes regarding metaphyseal bone strength, longitudinal bone growth and tensile strength of wounds. This may indicate reversion of the drug effects approximately one week after terminating medication.

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Contamination of wounds with fecal bacteria in immuno-suppressed mice

Karner

Drechsler

Metzger

et al. 2020

Sci Rep

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immunocompromised patients are predisposed to chronically infected wounds. especially ulcers in the dorsal region often experience secondary polymicrobial infections. However, current wound infection models mostly use single-strain bacteria. to mimic clinically occurring infections caused by fecal contamination in immunocompromised/immobile patients, which differ significantly from single-strain infections, the present study aimed at the establishment of a new mouse model using infection by fecal bacteria. Dorsal circular excision wounds in immunosuppressed mice were infected with fecal slurry solution in several dilutions up to 1:8,000. Impact of immunosuppressor, bacterial load and timing on development of wound infections was investigated. Wounds were analyzed by scoring, 3D imaging and swab analyses. Autofluorescence imaging was not successful. Dose-finding of cyclophosphamide-induced immunosuppression was necessary for establishment of bacterial wound infections. Infection with fecal slurry diluted 1:166 to 1:400 induced significantly delayed wound healing (p < 0.05) without systemic reactions. Swab analyses post-infection matched the initial polymicrobial suspension. The customized wound score confirmed significant differences between the groups (p < 0.05). Here we report the establishment of a simple, new mouse model for clinically occurring wound infections by fecal bacteria and the evaluation of appropriate wound analysis methods. in the future, this model will provide a suitable tool for the investigation of complex microbiological interactions and evaluation of new therapeutic approaches. Each year, 305 million people suffer from acute, traumatic or burn wounds, globally 1. The European community spends 2-4% of the total health expenditure on wound treatment 2. Wounds can arise from injuries, surgeries and as a consequence of extrinsic factors and underlying comorbidities (e.g. vascular diseases or diabetes). Hence, the general classifications differentiate between acute (e.g. burns or surgical wounds) and chronic wounds, (e.g. vascular, diabetic or pressure ulcers). In healthy individuals, the acute wounds generally heal without complications with basic supportive care and minimal infection risk. However, comorbidities and/or risk factors such as vascular diseases, diabetes, a weak immune system, bacterial colonization (especially with pathogens of high intrinsic virulence/resistance) may frequently lead to the development of a chronically infected wounds. Skin and soft tissue infections (SSTIs) represent the most common infections in humans 3. The associated impairment of wound healing incurs financial and logistic burden to the health care system and lowers the quality of life in affected patients 4. In the clinical setting, secondary infections of chronically non-healing wounds further aggravate their complex pathology and delay the healing processes. Immunocompromised patients are prone to develop chronically infected wounds 5 , and especially patients with pressure ulcers often experience seconda...

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Effects of Cyclophosphamide on Open, Granulating Skin Wounds in Rats

Cited by 5 publications

References 11 publications

Effects of Cyclophosphamide on the Formation and Solubility of Collagen

Effects of Cyclophosphamide on the Formation and Solubility of Collagen

Mechanical properties of bone and skin during recovery after cyclophosphamide administration

Contamination of wounds with fecal bacteria in immuno-suppressed mice

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