Effects of cyclooxygenase inhibitors on ex vivo cysteinyl-leukotriene production by whole human blood allowed to clot spontaneously. Comparison to stimulated blood

Investigations on the pharmacokinetic properties of Harpagophytum extracts and their effects on eicosanoid biosynthesis in vitro and ex vivo

“…The mixture was again centrifuged, and the supernatants were evaporated in a speed vac concentrator, resuspended in 10 mmol/L Tris-hydrochloric acid buffer (pH 7.4), and used for radioimmunologic measurement of Cys-LT in a validated assay as described earlier. 20 The antibody used primarily recognizes LTC 4 but shows a cross-reactivity of about 21% for LTD 4 . Because LTD 4 is enzymatically transformed from LTC 4 , the cross-reaction is of no relevance.…”