“…The mixture was again centrifuged, and the supernatants were evaporated in a speed vac concentrator, resuspended in 10 mmol/L Tris-hydrochloric acid buffer (pH 7.4), and used for radioimmunologic measurement of Cys-LT in a validated assay as described earlier. 20 The antibody used primarily recognizes LTC 4 but shows a cross-reactivity of about 21% for LTD 4 . Because LTD 4 is enzymatically transformed from LTC 4 , the cross-reaction is of no relevance.…”