Effects of Curcumin Chitosan Microspheres on the Expression of NF-κB, IL-1β, IL-4 and IL-6 in Rats with Ulcerative Colitis

Abstract

The aim of this study was to observe the therapeutic effect of curcumin chitosan microspheres (CCM) on ulcerative colitis (UC) in rats. Rat UC model was prepared by 2,4,6-trinitrobenzenesulfonic acid (TNBS) method. All animals were divided into blank control group (BCG), model control group (MCG), mesalazine enteric-coated tablets group (MECT), curcumin chitosan microsphere low (CCML), medium (CCMM) and high dose groups (CCMH). Starting from the third day after model establishment, the rats were intra-gastrically administered for 10 days. On the 14th day of the experiment, the rats were sacrificed. The colon tissues and serum samples were collected and the expression of NF-κB P65 protein was detected by immune histochemical streptavidin-perosidase (SP) method. The levels of IL-1β, IL-4 and IL-6 in serum were determined by Enzyme Linked Immunosorbent Assay (ELISA). The colonic mucosal injury score of MCG was increased; The serum IL-1β and IL-6 levels were increased, while the IL-4 content was decreased; The expression of NF-κB in the colonic mucosa was reduced after TNBS challenge. The histological injury scores of colonic mucosa, the serum levels of IL-1β and IL-6 in CCM-treated rats were significantly decreased (P < 0.01) as compared with MECT group. While, the content of IL-4 was significantly higher than that of MCG and MECT groups (P < 0.01). Curcumin chitosan microspheres provide a potential therapeutic strategy for treating UC in clinic.

Curcumin and Its Modified Formulations on Inflammatory Bowel Disease (IBD): The Story So Far and Future Outlook

“…In contrast, the IL-4 content was significantly higher than in the model control group (MCG) and MECT groups ( p < 0.01). The results showed that CCM offers a promising therapeutic approach for treating UC in a clinical study [ 117 ].…”

Biomaterial-based strategies for immunomodulation in IBD: current and future scenarios

“…These outcomes were noticeably better than those attained in mice given mesalazine tablets, a traditional IBD medication. 105 Similarly, ES100-coated and mesalamine-loaded chitosan microspheres also showed potential pH-dependent characteristics as chitosan NPs. 106…”

A tetrapeptide from maize combined with probiotics exerted strong anti-inflammatory effects and modulated gut microbiota in DSS-induced colitis mice

“…The mesalazine enteric-coated tablets were ground to powder, and then diluted with sterile water for oral administration in mice. 36 The intragastric doses of TPM and probiotics in mice were determined with reference to previous studies. 37,38 The doses of the TPM + probiotics group was selected according to the doses of TPM and probiotics, and both of them were given to mice at the same time, in order to better observe and compare the therapeutic effects in each group.…”