Effects of Cumin Extract on oxLDL , Paraoxanase 1 Activity , FBS, Total Cholesterol , Triglycerides , HDL-C , LDL-C , Apo A1 , and Apo B in in the Patients with Hypercholesterolemia

Abstract

Objectives: Paraoxanase 1 (PON1) plays a protective role against the oxidative modification of plasma lipoproteins and hydrolyzes lipid peroxides in human atherosclerotic lesions. Cumin is the dried seed of the herb Cuminumcyminum that is known as Zeera in Iran. Cumin seeds contain flavonoids which are now generally recognized to have antioxidant activity and improve the antioxidant system. So, they possibly modify PON1 activity and oxidized low density lipoprotein (oxLDL) level. The present study was aimed to evaluate the effects of cumin extract supplem entation on oxLDL, paraoxanase 1 activity, FBS, total cholesterol, triglycerides, High density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (Apo A1), and apolipoprotein B (Apo B)in the patients with hypercholesterolemia. Methodology:A fasting venous blood sample was obtained from the voluntary persons before and 45±3 days after taking cumin. Glucose, total cholesterol, and triglycerides were assayed using standard enzymatic procedures. HDL-Cand LDL-C were measured by direct method and ApoA1 and ApoB levels by immunoturbidimeteric methods. The levels of arylesterase and paraoxanase activities in the samples were measured by photometry methods and oxLDL by enzyme-linked immunosorbent assay (ELISA) method. 3 to 5 drops of cumin extract were added to the patient's diet three times a day based on manufacturer's instruction for 45±3 days. The biochemical param eters were compared before and after taking cumin. Data were analyzed using paired Student's t-test in SPSS statistical software (version 11.5). Results:The results dem onstrated that there was a significant decrease in the level of oxLDL after receiving cumin. Paraoxonase and arylesterase activities increased in serum after taking cumin extract. Conclusion:Based on the results, cumin reduces oxLDL level and increases both paraoxonase and arylesterase activity.

Effect of the cumin cyminum L. Intake on Weight Loss, Metabolic Profiles and Biomarkers of Oxidative Stress in Overweight Subjects: A Randomized Double-Blind Placebo-Controlled Clinical Trial

“…In agreement with our study, Samani et al . [26] reported that taking 3-5 drops of cumin extract three times a day among patients with hypercholesterolemia for 6 weeks did not affect lipid profiles. Furthermore, treatment with cumin cyminum methanol extract at the dose levels of 100 and 200 mg/day had no effect on serum levels of lipid profiles in male albino rats after 60 days [27].…”

Ameliorating effects of fennel and cumin extracts on sperm quality and spermatogenic cells apoptosis by inducing weight loss and reducing leptin concentration in diet-induced obese rats

“…demonstrated that cumin diminished oxidized low‐density lipoprotein (oxLDL) levels and ameliorate paraoxonase activity. They have stated that cumin seeds contain flavonoids which alter paraoxonase activity and oxidized oxLDL levels (Samani & Farrokhi, ).…”

The effect of cumin supplementation on metabolic profiles in patients with metabolic syndrome: A randomized, triple blind, placebo‐controlled clinical trial

“…Several in vitro studies have proposed beneficial effects of CuEO as an antioxidant and lipid peroxidation inhibitor (Chen et al, ; Hopps et al, ; Moghadam, ). A study revealed that CuEO significantly reduced the level of oxidized low‐density lipoprotein than the activities of paraoxonase, and arylesterase were enhanced (Samani & Farrokhi, ). Lipid peroxidation, which is caused by ROS, is one of the most important causes of cell damage.…”