Effects of cryopreservation methods on post-thaw motility of spermatozoa from the Japanese pearl oyster, Pinctada fucata martensii

Kawamoto, Takayuki; Narita, Teruyoshi; Isowa, Kiyoshi; Aoki, Hideo; Hayashi, Masahiro; Komaru, Akira; Ohta, Hiromi

doi:10.1016/j.cryobiol.2006.10.190

Cited by 39 publications

(38 citation statements)

References 20 publications

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“…However, the percentage decreased to 22.8% or less when concentrations of less than 8% DMSO were used. In the Japanese pearl oyster, DMSO was unsuitable because the motility of post‐thaw spermatozoa was low 4 . The 10% methanol + 18% FBS + 72% sea water mixture was an optimal cryopreservation diluent for the Japanese pearl oyster in terms of obtaining high post‐thaw motility 4 .…”

Section: Discussionmentioning

confidence: 99%

“…In the Japanese pearl oyster, DMSO was unsuitable because the motility of post‐thaw spermatozoa was low 4 . The 10% methanol + 18% FBS + 72% sea water mixture was an optimal cryopreservation diluent for the Japanese pearl oyster in terms of obtaining high post‐thaw motility 4 . Although we have not yet examined the effects of concentration and equilibration time of DMSO and other cryoprotectants on the structure of Japanese pearl oyster sperm, comparative observations on spermatozoa cryopreserved with these cryoprotectants should be carried out in subsequent studies.…”

Section: Discussionmentioning

confidence: 99%

“…We previously reported an effective cryopreservation method of spermatozoa that obtained a maximal post‐thaw motility 4 and fertilization rate 5 in the Japanese pearl oyster. The motility of cryopreserved spermatozoa was approximately 30% of that of fresh spermatozoa 4 . In artificial fertilization experiments, approximately 10 times the volume of cryopreserved spermatozoa is required to obtain equivalent fertilization rates by fresh spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

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Effects of cryopreservation on sperm structure in Japanese pearl oysterPinctada fucata martensii

et al. 2008

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To clarify factors reducing the motility and fertility of cryopreserved spermatozoa of the Japanese pearl oyster Pinctada fucata martensii, the structure of spermatozoa before and after cryopreservation was observed by scanning electron microscopy. Testicular spermatozoa were diluted with cryopreservation diluent (10% methanol + 18% fetal bovine serum + 72% sea water), and dispensed into 0.25-mL straws. The straws were cooled at a rate of approximately -20°C/min to -50°C, and subsequently immersed in liquid nitrogen. Percentage motility of spermatozoa before cryopreservation was 69.9 Ϯ 4.2%, and that of cryopreserved spermatozoa was 24.0 Ϯ 1.8%, respectively. In cryopreserved spermatozoa, the percentage that lacked or had a deformed flagellum was 56.6 Ϯ 3.9%, while in fresh spermatozoa this was 8.7 Ϯ 2.0%. In cryopreserved spermatozoa, the percentage of deformed acrosomes was 76.6 Ϯ 5.2%, while in fresh spermatozoa this was only 0.9 Ϯ 0.3%. Cryopreserved spermatozoa with a normal acrosome and flagellum were only 15.4 Ϯ 3.5% of those in fresh spermatozoa. These results indicate that lesion of the flagellum and deformation of the acrosome occurred through the cryopreservation procedure, and both types of damage lead to loss of the motility and fertility in thawed spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of cryopreservation on sperm structure in Japanese pearl oysterPinctada fucata martensii

et al. 2008

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“…During this phase, sperm survival depends on intracellular ice formation and salt concentration, both parameters being modified by the movement of water out of the cell as the temperature decreased (Cloud and Patton 2009). Post thaw motility of Japanese pearl oyster (Pinctada fucata martensii) sperm was lower than 5% when straws were maintained at 7.5 cm above LN and increased abruptly to 35-40% at 10 cm (Kawamoto et al 2007). Compared to great scallop sperm, a close height above LN of 5.2 cm was used for greenlip abalone (Haliotis laevigata, Liu et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of great scallop (Pecten maximus) sperm: effect of extender, cryoprotectant and cooling rate on sperm survival

Suquet

Arsenault-Pernet

Ratiskol

et al. 2014

Aquat. Living Resour.

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-To develop selection programs for the great scallop, artificial reproduction of the species needs to be improved by gamete cryobanking. Here, a set of four experiments was designed in order to define the basic elements of a cryopreservation protocol for scallop sperm, including extender composition (experiment 1), cryoprotectant selection (2), cooling rate (3), and assessment of the effects of sperm cryopreservation on sperm motility and fertilization capacity (4). Sperm was collected after serotonin injection (100 µl of a 10-mM solution) and frozen in 500-µl straws. For the first three experiments, the percentage of motile fresh sperm (80 ± 4%, mean ± SEM) was significantly higher than that observed for thawed sperm (11 ± 2%). During the first experiment, no significant difference of the percentage of motile thawed sperm was observed among the three saline extenders tested: seawater, calcium-free Hanks' balanced salt solution (Ca-free HBSS) and DCSB4 solution. However, a complementary experiment (2) showed that a significantly higher percentage of motile thawed sperm was recorded using DCSB4 than with Ca-free HBSS as an extender. During the third experiment, sperm motility was higher when using polyethylene glycol (10% PEG) as a cryoprotectant, than when using dimethyl sulphoxide, DMSO (10 and 20%), methanol (10%) and ethylene glycol (10% EG). A higher D-larval rate was obtained during the fourth experiment, using fresh sperm than thawed sperm, and using a 500:1 sperm-to-egg ratio compared with a 50:1 ratio. There is some evidence of inter-individual variations in sperm tolerance to cryopreservation. In conclusion, the highest survival of great scallop sperm after thawing was recorded using the following conditions: DCSB4 extender (1:3 vol/vol sperm to extender dilution), PEG cryoprotectant (10%) and straws maintained at 5.5 cm above liquid nitrogen.

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“…Several species of oysters have been the main focus of such studies: pearl oyster ( Pinctada margaritifera ) (Acosta-Salmón et al, 2007); Japanese pearl oyster ( Pinctada fucata martensii ) (Kawamoto et al, 2007); Pacific oyster ( Crassostrea gigas ) (Dong et al, 2005, 2006); Eastern oyster ( Crassotrea virginica ) (Paniagua-Chavez and Tiersch, 2001); Ostrea edulis (Vitiello et al, 2011) and Portuguese oyster ( Crassostrea angulata ) (Riesco et al, in press). Different studies on cryopreservation and cold storage of spermatozoa from Echinoderms have also been performed during more than three decades (Adams et al, 2004; Dunn and McLachlan, 1973; Spiegler and Oppenheimer, 1995).…”

Section: Germplasm Cryobanking Of Invertebratesmentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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Effects of cryopreservation methods on post-thaw motility of spermatozoa from the Japanese pearl oyster, Pinctada fucata martensii

Cited by 39 publications

References 20 publications

Effects of cryopreservation on sperm structure in Japanese pearl oysterPinctada fucata martensii

Effects of cryopreservation on sperm structure in Japanese pearl oysterPinctada fucata martensii

Cryopreservation of great scallop (Pecten maximus) sperm: effect of extender, cryoprotectant and cooling rate on sperm survival

Cryobanking of aquatic species

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