Effects of craniopharyngioma cyst fluid on neurons and glial cells cultured from rat brain hypothalamus

Adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β-amyloid

“…Pseudochronological analysis suggested that activated microglia mainly exhibit inflammation activation, increased synthesis of proinflammatory factors, activation of inflammation-related pathways, increased response to lipoprotein particle stimulation, and beta-amyloid (Aβ) synthesis and response. The signal that positively regulates neuronal apoptosis is significantly strengthened in ARC neurons, which is similar to the results of in vitro experiments previously reported by Ghosh et al [ 24 ]. It is worth noting that beta-amyloid (Aβ) produced by APP, a pathological sign of Alzheimer's disease, causes inflammatory damage to neurons and is responsible for the degeneration of synapses and the apoptosis of neurons [ 38 ].…”

“…It was previously reported that ACP cyst fluid may have a direct toxic effect on neurons [ 24 ], and we also believe that various types of glial cells in the hypothalamic tissue have an effect on neurons. Through further exploration of the single-cell RNA sequencing data, we unexpectedly found that the cell number of cluster 11 microglia in the cystic fluid group was far greater than that in the sham operation group.…”

“…As previously shown, ACP cystic fluid has a damaging effect on neurons in vitro [ 24 ] ; the abovementioned gene changes may be caused by the direct damage of cystic fluid to ARC neurons. However, through pseudochronological analysis and GO pathway enrichment analysis, we found that ACP cystic fluid caused inflammatory activation of microglia and increased expression of IL-6, which was also verified in PCR experiments.…”

“…It has been reported that the leakage of cyst fluid can cause severe chemical meningitis [ 22 ], and it was previously reported that the injection of cyst fluid into the cortex of rats caused increased expression of markers of inflammation and cell damage and increased body weight [ 23 ]. In vitro experiments have shown that cyst fluid can cause degenerative changes and apoptosis of neurons [ 24 ]. More and more data proved that ACP cystic fluid may be a key factor of hypothalamic inflammatory injury.…”

Adamantinomatous Craniopharyngioma Cyst Fluid can Trigger Inflammatory Activation of Microglia to Damage the Hypothalamic Neurons by Inducing the Production of β-Amyloid

“…It has been reported that the leakage of cyst uid can cause severe chemical meningitis [22], and it was previously reported that the injection of cyst uid into the cortex of rats caused increased expression of markers of in ammation and cell damage and increased body weight [23]. In vitro experiments have shown that cyst uid can cause degenerative changes and apoptosis of neurons [24]. More and more data proved that ACP cystic uid may be a key factor of hypothalamic in ammatory injury .However, there is currently no reliable ACP cystic uid-hypothalamus injury animal model for in-depth study of the speci c mechanism by which cyst uid induces hypothalamic injury .The effect of cystic uid which is the product of ACP lipid metabolism disorder, on the hypothalamus is still unclear.…”