Effects of coronatine elicitation on growth and metabolic profiles of Lemna paucicostata culture

Abstract

In this study, the effects of coronatine treatment on the growth, comprehensive metabolic profiles, and productivity of bioactive compounds, including phenolics and phytosterols, in whole plant cultures of Lemna paucicostata were investigated using gas chromatography-mass spectrometry (GC-MS) coupled with multivariate statistical analysis. To determine the optimal timing of coronatine elicitation, coronatine was added on days 0, 23, and 28 after inoculation. The total growth of L. paucicostata was not significantly different between the coronatine treated groups and the control. The coronatine treatment in L. paucicostata induced increases in the content of hydroxycinnamic acids, such as caffeic acid, isoferulic acid, ρ-coumaric acid, sinapic acid, and phytosterols, such as campesterol and β-sitosterol. The productivity of these useful metabolites was highest when coronatine was added on day 0 and harvested on day 32. These results suggest that coronatine treatment on day 0 activates the phenolic and phytosterol biosynthetic pathways in L. paucicostata to a greater extent than in the control. To the best of our knowledge, this is the first report to investigate the effects of coronatine on the alteration of metabolism in L. paucicostata based on GC-MS profiling. The results of this research provide a foundation for designing strategies for enhanced production of useful metabolites for pharmaceutical and nutraceutical industries by cultivation of L. paucicostata.

Enhanced Production of Photosynthetic Pigments and Various Metabolites and Lipids in the Cyanobacteria Synechocystis sp. PCC 7338 Culture in the Presence of Exogenous Glucose

“…Five milligrams of each Synechocystis 7338 sample from days 9 and 18 were weighed and used for metabolic profiling by GC-MS, according to a previously described method [ 33 ]. A gas chromatography system (7890A, Agilent Technologies, CA, USA) equipped with a mass selective detector (5975C, Agilent Technologies) and an autosampler (7683B, Agilent Technologies) was used in the present study.…”

“…To process the raw data from GC-MS and nanoESI-MS analyses and identify metabolites and intact lipid species, we used previously described methods [ 33 , 34 ]. The Student’s t -test and Mann–Whitney test were used to evaluate significant differences in relative level (relative intensity/g) obtained from GC-MS and nanoESI-MS.…”

Metabolic and Lipidomic Profiling of Vegetable Juices Fermented with Various Probiotics

“…Of this, 200 µL were transferred to GC vials and dried under nitrogen for 20 min. The derivatization and GC-MS analysis of each sample were conducted according to previously reported methods [20]. A split ratio of 1:15 was used, and the detector voltage was set to 1153 V. The oven temperature was set at 60 • C and programmed to increase to 185 • C at 5 • C/min (hold time 3 min), then to 205 • C at 3 • C/min, and finally to 310 • C at 5 • C/min.…”

Alteration of metabolic profiles in Lemna paucicostata culture and enhanced production of GABA and ferulic acid by ethephon treatment

“…Samples were ground before performing gas chromatography-mass spectrometry (GC-MS) analysis; the extraction method was based on Kim et al [26]. As an internal standard (IS), 10 μL of 2,000 mg/L myristic-d 27 acid (Tokyo Chemical Industry Co., Japan) in pyridine was used.…”