Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis Tissue

Abstract

The aim of this study is to investigate the effects of Protocatechuic acid and Corchorus olitorius on streptozotocin (STZ) induced diabetic rat testis tissue. Randomly selected Wistar Albino rats were divided into five groups as; Diabetes Mellitus, Diabetes Mellitus treated with Corchorus Olitorus (STZ+CO), Diabetes Mellitus treated with Protacatechuic acid (STZ+PCA), Corchorus olitorius (CO), Protocatechuic acid (PCA) and Control. Diabetic model was generated by intraperitoneal injection of 60 mg/kg Streptozotosin. After 48 hours of the STZ injection, blood samples were collected from tail vein in order to measure blood glycose levels. Over 250 mg/dL accepted as diabetic subjets and fed with 250 mg/kg Corchorus olitorius or 20 mg/kg PCA by oral gavage for three weeks. At the end of the experiment, right testes were removed and fixed in 10 % neutral formaldehyde for paraffine embedding. Sections were stained by HE, Masson trichrome, PAS and TUNEL for microscopic evaluation. Control, PCA-only and Corchorus olitorius-only treated group testes tissues showed a normal tissue organization, when degeneration in seminiferous tubules, the vacuolization, seperations in spermatogenic cell series, outpouring of cell groups in the lumen, vesicular body formation, liquid accumulation in the interstitial region and edema were observed in STZ induced diabetic models and untreated groups. Besides, higher amount of TUNEL (+) stained cells were determined in STZ group. On the other hand, blood glucose level and number of TUNEL (+) stained cells were decreased as a result of PCA and Corchorus olitorius treatment. Because of the reduction of blood glucose level and apoptotic cell numbers, PCA and Corchorus olitorius decreace the complications of diabetes mellitus induced rat testis

Corchorus olitorius extract exhibit anti-hyperglycemic and anti-inflammatory properties in rodent models of obesity and diabetes mellitus

“…For example, Anyasor et al ( 50 ) demonstrated that using 80 g ethanolic extract of C. olitorius leaf for 13 days in T2D rats could improve glucose metabolism, as revealed by a significant decrease in plasma glucose in the treated group (99.0 mg/dL) compared to 313.5 mg/dL, p < 0.05 in the control group. Similarly, Mercan et al ( 52 ) reported that the administration of 96% ethanolic extract of C. olitorius (250 mg/kg) for 3 weeks in STZ-induced T2D Wistar albino rats could significantly decrease FBG (297.83 mg/dL) when compared to untreated STZ rats (419.00 mg/dL, p < 0.05). The same study revealed a 0.44-fold decrease in FBG when baseline and post-treatment results were compared.…”

A Comprehensive Review of C. capsularis and C. olitorius: A Source of Nutrition, Essential Phytoconstituents and Pharmacological Activities

“…A group of researchers studied the effect of C. olitorius in structural changes of testicular tissue in streptozotocin-induced diabetic rats [ 126 ]. They found that blood glucose levels significantly decreased in the treatment groups compared with the diabetic group; seminiferous tubule degenerations were prevented, and apoptotic cell numbers were reduced.…”