Effects of Coptis chinensis Extracts on Matrix Metalloproteinase-1 Suppression through JNK/AP-1 Axis in Human Dermal Fibroblasts

Abstract

Purpose: In this study, we investigate the effect of Coptis chinensis extract (CCE) on matrix metalloprotease 1 (MMP1) expression in dermal fibroblasts. Methods: Human dermal fibroblasts (HDFs) were used to assess the effect of CCE on oxidative stress-induced MMP1 expression. A water-soluble tetrazolium salt (WST-1) assay was conducted to determine the cytotoxicity of CCE in HDFs. To investigate whether CCE has a protective effect on oxidative stress-induced MMP1 downregulation, HDFs were pretreated with CCE (2.5 and 5 µg/mL) for 9 h, and then with 200 µM H 2 O 2 for 3 h. Total RNAs were collected and the level of MMP1 expression was determined using quantitative real-time polymerase chain reaction (qRT-PCR) assay with its specific primers. Also, luciferase reporter gene assay for activator protein-1 (AP-1) activity was performed with AP-1 luciferase reporter gene constructs transfected into HDFs. Furthermore, c-Jun N-terminal kinase (JNK) and p-JNK protein levels were detected by western blot analysis. Results: Lower concentration of CCE (<5 µg/mL) showed little cytotoxicity in HDFs. Pre-treatment of the cells with CCE significantly inhibited H 2 O 2-induced MMP1 downregulation. Further experiments revealed that CCE-mediated MMP1 downregulated was mediated via suppression of AP-1 transcriptional activity. Moreover, western blot analysis revealed that CCE inhibited the phosphorylation and activation of JNK, which is known to enhancing AP-1 activation. Therefore, these results indicate that CCE regulates MMP1 expression via JNK/AP-1 signaling pathway in HDFs. Conclusion: These results in this paper show that CCE has a protective effect on oxidative stress-induced MMP1 expression in HDFS and may uses as a possible reagent on anti-aging cosmetics.

Suppression of Matrix Metallopeptidase-3 Expression in Human Dermal Fibroblasts by Decursin from Angelica gigas Nakai Root Extracts Fermented with Jeju Lava Seawater

“…http://www.e-ajbc.org 다 (Philips et al, 2011). 콜라겐분해효소(collagenase)에 속하는 MMP1, MMP8, MMP13은 콜라겐 타입 I, 타입 III의 분해에 관여 하며, MMP1이 가장 주요하게 콜라겐을 분해한다 (Brennan et al, 2003;Kim et al, 2018b). Stromelysins 중 하나인 MMP3은 광범 위하게 콜라겐을 포함한 세포외 기질의 단백질들(프로테오글리칸, 기저막 콜라겐, 매트릭스 당 단백질)을 분해하는 효소이다 (Philips et al, 2011).…”