Effects of copper and cadmium on cholinesterase and glutathione S-transferase activities of two marine gastropods (Monodonta lineata and Nucella lapillus)

Cunha, Isabel; Mangas-Ramírez, Ernesto; Guilhermino, Lúcia

doi:10.1016/j.cbpc.2007.02.014

Cited by 68 publications

(47 citation statements)

References 55 publications

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“…Copper and Cadmium have been of great concerns in marine ecosystems since these two heavy metals could be bioaccumulated in the body tissues by marine organisms (Cunha et al, 2007). Exposure to Cu and Cd was shown to induce the expression of GST transcripts in many aquatic organisms (Yoshinaga et al, 2007;Lee et al, 2008;Kim et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Zhang

Liu

et al. 2011

Chin. J. Ocean. Limnol.

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Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxifi cation of xenobiotics, and also play important roles in antioxidant defense. We identifi ed two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profi les of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these fi ndings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.

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Section: Discussionmentioning

confidence: 99%

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Zhang

Liu

et al. 2011

Chin. J. Ocean. Limnol.

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“…A dose and time response both in phagocytic activity of haemocytes and lysosomal structural in the Asian clam (Corbicula fluminea) exposed to 10 g L −1 of cadmium demonstrated their use as biomarkers in freshwater biomonitoring [98]. The enzymes cholinesterases (ChEs) showing properties of both typical acetylcholinesterase and pseudocholinesterase in foot muscle of M. lineate and N. lapillus were increased by cadmium in vivo exposure and inhibited by copper in vitro exposure [94]. Some new biomarkers are now being explored for feasibility of its practical use.…”

Section: Biochemical Alterationsmentioning

confidence: 99%

“…Biochemical stress of glutathione (GSH) levels in aquatic moss Fontinalis species showed alterations due to Cd 2+ exposure over 10-day period [93]. Test of GST activity in two marine gastropods (Monodonta lineate and N. lapillus) exposed to copper and cadmium showed a significant reduction of N. lapillus GST after copper exposure but had no effect on M. lineate GST, indicating the selection of biochemical biomarkers should consider of the experimental species and tested pollutants [94].…”

Section: Biochemical Alterationsmentioning

confidence: 99%

Biomonitoring: An appealing tool for assessment of metal pollution in the aquatic ecosystem

Zhou

Zhang

et al. 2008

Analytica Chimica Acta

756

348

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“…Copper and cadmium have the capability to induce toxic effects in living organisms, due to bioaccumulation. In the past years, GST has been used as environmental biomarker because its important role in detoxification and antioxidative stress defenses [13]. Regarding the effect of metals in GST activity, there are contradictory effects and diverse mechanism of action reported.…”

Section: Introductionmentioning

confidence: 99%

Inhibition by Cu²⁺ and Cd²⁺ of a mu‐class glutathione S‐transferase from shrimp Litopenaeus vannamei

Salazar-Medina

García-Rico

García-Orozco

et al. 2010

J Biochem & Molecular Tox

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Glutathione S-transferases (GSTs) are a family of detoxifying enzymes that catalyze the conjugation of glutathione (GSH) to electrophiles, thereby increasing the solubility of xenobiotics and aiding its excretion from the cell. The present work presents the inhibition of a mu-class GST of the marine shrimp Litopenaeus vannamei by copper (Cu2+) and cadmium (Cd2+). The protein was overexpressed in bacteria and its enzymatic activity measured using 1-chloro-2,4-dinitrobenzene. The mean inhibitory concentration (IC(50)) for shrimp GST against Cu2+) was 4.77 microM and for Cd2+ was 0.39 microM. A molecular model of the protein based on the crystal structure of a maize GST bound to cadmium showed that the metal binds in the GSH-binding site by coordination with Asp and Gln residues. These results are consistent with the experimental data and suggest that sublethal concentration of metals may affect the capacity of the organism to detoxify pesticides or xenobiotics.

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Effects of copper and cadmium on cholinesterase and glutathione S-transferase activities of two marine gastropods (Monodonta lineata and Nucella lapillus)

Cited by 68 publications

References 55 publications

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Biomonitoring: An appealing tool for assessment of metal pollution in the aquatic ecosystem

Inhibition by Cu²⁺ and Cd²⁺ of a mu‐class glutathione S‐transferase from shrimp Litopenaeus vannamei

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Effects of copper and cadmium on cholinesterase and glutathione S-transferase activities of two marine gastropods (Monodonta lineata and Nucella lapillus)

Cited by 68 publications

References 55 publications

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Biomonitoring: An appealing tool for assessment of metal pollution in the aquatic ecosystem

Inhibition by Cu2+ and Cd2+ of a mu‐class glutathione S‐transferase from shrimp Litopenaeus vannamei

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Inhibition by Cu²⁺ and Cd²⁺ of a mu‐class glutathione S‐transferase from shrimp Litopenaeus vannamei