Effects of conjugated linoleic acid on liver composition and fatty acid oxidation are isomer-dependent in hamster

Macarulla, M. T.; Fernández‐Quintela, Alfredo; Zabala, Alazne; Navarro, Virginia; Echevarrı́a, Enrique; Churruca, Itziar; Rodríguez, Víctor M.; Portillo, Marı́a P.

doi:10.1016/j.nut.2004.07.011

Cited by 50 publications

(51 citation statements)

References 38 publications

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“…It shows that the decreases in triglycerides may have resulted through involvement of acetyl-CoA oxidase due to a PPARα ligand binding activation. It has been reported that the addition of 0.5% of trans-10, cis-12 conjugated linoleic acid isomer to the diet increases liver fatty acid oxidation leading to decreased hepatic and serum triacylglycerols [17]. Stimulation of acetyl-CoA oxidase by alpha-linolenic acid-rich perilla oil was reported to lower plasma triacylglycerol level in rats [18].…”

Section: Resultsmentioning

confidence: 99%

Dietary oxidized linoleic acid lowers triglycerides via APOA5/APOClll dependent mechanisms

et al. 2008

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Previously we have shown that intestinal cells efficiently take up oxidized fatty acids (OxFAs) and that atherosclerosis is increased when animals are fed a high cholesterol diet in the presence of oxidized linoleic acid. Interestingly, we found that in the absence of dietary cholesterol, the oxidized fatty acid fed low-density lipoprotein (LDL) receptor negative mice appeared to have lower plasma triglyceride (TG) levels as compared to animals fed oleic acid. In the present study, we fed C57BL6 mice a normal mice diet supplemented with oleic acid or oxidized linoleic acid (at 18 mg/animal/ day) for 2 weeks. After the mice were sacrificed, we measured the plasma lipids and collected livers for the isolation of RNA. The results showed that while there were no significant changes in the levels of total cholesterol and high-density lipoprotein cholesterol (HDLc), there was a significant decrease (41.14%) in the levels of plasma TG in the mice that were fed oxidized fatty acids.The decreases in plasma TG levels were accompanied by significant increases (P < 0.001) in the expressions of APOA5 and acetyl-CoA oxidase genes as well as a significant (P < 0.04) decrease in APOClll gene expression. Oxidized lipids have been suggested to be ligands for peroxisome proliferator-activated receptor (PPARα). However, there were no increases in the mRNA or protein levels of PPARα in the oxidized linoleic acid fed animals. These results suggest that oxidized fatty acids may act through an APOA5/APOClll mechanism that contributes to lowering of TG levels other than PPARα induction.

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Section: Resultsmentioning

confidence: 99%

Dietary oxidized linoleic acid lowers triglycerides via APOA5/APOClll dependent mechanisms

et al. 2008

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“…Although the beneficial effects of CLA and possibly CLnA in hamsters seem to be isomer-dependent, other experimental conditions and design could also have had a major influence on the results (Yoganathan et al, 1998;Dorfman et al, 2003;Macarulla et al, 2005;Wilson et al, 2006). For example, gene expression for receptors is sensitive to the hamster strain and to the lipid composing the diet (Loison et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…CLA may also decrease the formation of fatty streaks in hamsters and rabbits (Lee et al, 1994;Kritchevsky et al, 2004) and may help to attenuate atherosclerosis by inducing apoptosis in the atherosclerotic lesion in mice (Toomey et al, 2006). However, a recent study on hamsters reported that there may be an isomer-dependent CLA effect in this rodent model (Macarulla et al, 2005).…”

Section: Introductionmentioning

confidence: 95%

Adverse effects of conjugated alpha-linolenic acids (CLnA) on lipoprotein profile on experimental atherosclerosis in hamsters

Plourde

Ledoux²,

Grégoire³

et al. 2007

Animal

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Conjugated linoleic acids (CLAs) such as rumenic acid (RA) have the potential to alter blood lipid profiles in animals and in humans. In contrast, physiological effects of conjugated a-linolenic acids (CLnAs), which concomitantly are omega-3 and conjugated fatty acids, are still unknown. The aim of this study was to evaluate the potential of CLnA to interfere in early steps of atherosclerosis by altering lipoprotein profiles and fatty streaks in the aortas. F 1 B hamsters were fed a control or one of the three hypercholesterolemic (HC) diets: HC-control, HC-RA (18:2 cis-9, trans-11) or HC-CLnA (CLnA: equimolar mixture of 18:3 cis-9, trans-11, cis-15 and cis-9, trans-13, cis-15) diet. In low-cholesterol control-fed hamsters, the proportion of high-density lipoprotein cholesterol (HDL-C) was around 45% while in HC-fed hamsters, HDL-C was around 10% and cholesterol was mostly (80%) carried by triglyceride-rich lipoproteins (TRL). Low-density lipoprotein (LDL) triglycerides (TGs) increased by approximately 60% in hamsters fed either HC-RA or HC-CLnA compared with HC-controls but not compared with the low-cholesterol control diet. HDL cholesterol decreased by 24% and 16% in hamsters fed HC-RA and HC-CLnA, respectively. Small dense LDLcholesterol increased by approximately 60% in hamsters fed HC-RA and HC-CLnA compared with the HC-control group and by more than a 100% compared with hamsters on the control diet. The relative percentage of liver cholesteryl ester content increased by 88% in hamsters fed HC diets compared with the control diet. Significant differences in fatty streaks were observed between control and HC-diet-fed hamsters. However, no significant difference was observed among the HC-diet-fed hamsters. This study shows that animals fed any one of the HC diets developed an adverse lipoprotein profile compared with a normolipidic diet. Also, HC-RA or HC-CLnA diets altered lipoprotein profile compared with animals fed the HC-control diet but had no beneficial effects on atherosclerosis.

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“…O fato de observar mais glóbulos de lípídios nos fígados dos ratos que consumiram CLA caracteriza uma informação de natureza qualitativa, no entanto não menos importante, já que permite conclusões sobre a integridade das células hepáticas. Anormalidades na morfologia hepática acompanhadas do acúmulo de lípides em animais suplementados com CLA têm sido reportadas por alguns pesquisadores 14,29 , enquanto outros observam ou não o acúmulo de lípides sem alterar a morfologia do órgão 5,30 . Nas condições de suplementação do presente trabalho não se observou acúmulo de lípides hepáticos e tampouco alteração da morfologia do órgão dos ratos.…”

Section: I S C U S S ã Ounclassified

Suplementação com ácido linoléico conjugado: estabilidade oxidativa dos suplementos e correlações com conteúdo dos lípides totais hepáticos e indicadores da oxidação dos lípides biológicos de ratos Wistar

2009

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OBJETIVO:O objetivo do trabalho foi avaliar a estabilidade oxidativa de misturas comerciais de ácido linoléico conjugado e buscar possível correlação entre a suplementação e o conteúdo total de lípides hepáticos, e também de alguns indicadores da oxidação lipídica em ratos. MÉTODOS:Um ensaio biológico com 30 ratos divididos em três grupos (n=10) caracterizando os grupos controle e suplementados com as misturas comerciais AdvantEdge® e One® foi realizado. A concentração administrada foi de 2% em relação ao consumo de dieta e os animais foram suplementados durante 42 dias. O conteúdo total de lípides do fígado foi determinado e a morfologia do órgão foi examinada por meio de microscopia ótica. Índice de peróxido e malondialdeído foram determinados para avaliar a estabilidade oxidativa dos suplementos in vitro. Índice de peróxido, malondialdeído, 8-iso-PGF2α isoprostana e catalase foram determinados como indicadores da oxidação dos lípides biológicos. RESULTADOS: Os resultados demonstraram baixa estabilidade das misturas comerciais à oxidação in vitro. As associações entre o consumo de ácido linoléico conjugado e malondialdeído (r=-0,7914, p<0,0001) e catalase (r=-0,5991, p=0,008) foram moderadas, negativas e significantes, demonstrando que o ácido linoléico conjugado reduziu a oxidação lipídica in vivo. O conteúdo dos lípides totais hepáticos não aumentou (22,42%, DP=1,40%) e a morfologia do órgão permaneceu íntegra. CONCLUSÃO:Embora esse protocolo de suplementação com ácido linoléico conjugado tenha reduzido a oxidação lipídica, há que considerar a tendência para o aumento de 8-iso-PGF2α isoprostana e de peróxidos como perspectiva de continuidade das pesquisas sobre a reinvestigação do efeito antioxidante do ácido linoléico conjugado.

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Effects of conjugated linoleic acid on liver composition and fatty acid oxidation are isomer-dependent in hamster

Cited by 50 publications

References 38 publications

Dietary oxidized linoleic acid lowers triglycerides via APOA5/APOClll dependent mechanisms

Dietary oxidized linoleic acid lowers triglycerides via APOA5/APOClll dependent mechanisms

Adverse effects of conjugated alpha-linolenic acids (CLnA) on lipoprotein profile on experimental atherosclerosis in hamsters

Suplementação com ácido linoléico conjugado: estabilidade oxidativa dos suplementos e correlações com conteúdo dos lípides totais hepáticos e indicadores da oxidação dos lípides biológicos de ratos Wistar

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