Effects of Compressed Hydrocarbon Gases on the Growth Activity of Saccharomyces cerevisiae

Abstract

The inhibitory action of compressed hydrocarbon gases on the growth of the yeast Saccharomyces cerevisiae was investigated quantitatively by microcalorimetry. Both the 50% inhibitory pressure (IP(50)) and the minimum inhibitory pressure (MIP), which are regarded as indices of the toxicity of hydrocarbon gases, were determined from growth thermograms. Based on these values, the inhibitory potency of the hydrocarbon gases increased in the order methane << ethane < propane < i-butane < n-butane. The toxicity of these hydrocarbon gases correlated to their hydrophobicity, suggesting that hydrocarbon gases interact with some hydrophobic regions of the cell membrane. In support of this, we found that UV absorbing materials at 260 nm were released from yeast cells exposed to compressed hydrocarbon gases. Additionally, scanning electron microscopy indicated that morphological changes occurred in these cells.

Microbial Reduction and Quality Changes in Powdered White and Black Pepper by Treatment with Compressed Oxygen or Carbon Dioxide Gas

“…The use of pressurized gas has begun to attract attention for its antimicrobial action (Erkmen, 2000;Spilimbergo et al, 2003;Muramoto et al, 2004;Garcia-Gonzalez et al, 2007;Tamura, 2007;Ferrentino et al, 2009;Garcia-Gonzalez et al, 2009;Calvo and Torres, 2010;Kawachi et al, 2010;Ferrentino and Spilimbergo, 2011). Recently, we demonstrated that yeasts in sudachi juice, a citrus juice, could be inactivated with pressurized oxygen at 10 MPa and 50℃ without color alteration or significant loss of vitamin C or limonene (Muramoto et al, 2004;Tamura, 2007).…”

Effects of Gas Pressurization with Ethylene on the Ultrastructure of the YeastSaccharomyces cerevisiae

“…It was also found that pressurization with hydrocarbon gas induced deformity of the cell surface in small numbers of yeast cells. 2,3) We expect to find that gas pressurization with hydrocarbon impairs intracellular organelles in yeast. Little information is available on changes in intracellular organelles induced by gas pressurization, especially with ethylene.…”

“…It is often used as a model eukaryote for rapid and easy bioassay. [2][3][4][5][6] Saccharomyces cerevisiae IFO10149 was grown at 30 C in YPD medium (20 g/L glucose, 20 g/L peptone, and 10 g/L yeast extract) until it reached normal log-phase growth (O.D. at 660 nm, 0.9).…”