Effects of Composition and Mechanical Property of Injectable Collagen I/II Composite Hydrogels on Chondrocyte Behaviors

Lu, Yuan; Li, Bao; Yang, Jun; Ni, Yilu; Teng, Ying-Ying; Guo, Likun; Fan, Hongsong; Fan, Yujiang; Zhang, Xingdong

doi:10.1089/ten.tea.2015.0513

Cited by 67 publications

(40 citation statements)

References 41 publications

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“…101,102,103,104 There are at least 19 types of collagen, such as type I, type II, type III, and type V. 101 Recently, naturally derived collagen has been widely used to construct collagen-based scaffolds for various biomedical applications, particularly tissue engineering, because it has the favorable property of being weakly antigenic. 8,49,105 Yuan et al 105 have combined type I and type II collagens to construct a favorable injectable hydrogel whose compressive modulus can be regulated by changing the type I collagen content in the hydrogel. The chondrocytes embedded in the hydrogel maintain their natural morphology and secrete cartilage-specific ECM.…”

Section: Injectable Hydrogels Prepared With Different Biomaterialsmentioning

confidence: 99%

Injectable hydrogels for cartilage and bone tissue engineering

et al. 2017

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Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for use as three-dimensional cell culture scaffolds in cartilage and bone tissue engineering, owing to their high water content, similarity to the natural extracellular matrix (ECM), porous framework for cell transplantation and proliferation, minimal invasive properties, and ability to match irregular defects. In this review, we describe the selection of appropriate biomaterials and fabrication methods to prepare novel injectable hydrogels for cartilage and bone tissue engineering. In addition, the biology of cartilage and the bony ECM is also summarized. Finally, future perspectives for injectable hydrogels in cartilage and bone tissue engineering are discussed.

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Section: Injectable Hydrogels Prepared With Different Biomaterialsmentioning

confidence: 99%

Injectable hydrogels for cartilage and bone tissue engineering

et al. 2017

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“…The composition and mechanical properties of cryogel scaffolds could influence chondrocyte behavior [26]. In this paper, we aimed to investigate the dual function of GlcN when it is incorporated into gelatin/HA (GH) cryogel scaffolds, which can affect cell functions and induce tissue regeneration through biological and physical cues.…”

Section: Introductionmentioning

confidence: 99%

Dual Function of Glucosamine in Gelatin/Hyaluronic Acid Cryogel to Modulate Scaffold Mechanical Properties and to Maintain Chondrogenic Phenotype for Cartilage Tissue Engineering

Chen

Kuo

Wang

et al. 2016

IJMS

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Glucosamine (GlcN) fulfills many of the requirements as an ideal component in scaffolds used in cartilage tissue engineering. The incorporation of GlcN in a gelatin/hyaluronic acid (GH) cryogel scaffold could provide biological cues in maintaining the phenotype of chondrocytes. Nonetheless, substituting gelatin with GlcN may also decrease the crosslinking density and modulate the mechanical properties of the cryogel scaffold, which may be beneficial as physical cues for chondrocytes in the scaffold. Thus, we prepared cryogel scaffolds containing 9% GlcN (GH-GlcN9) and 16% GlcN (GH-GlcN16) by carbodiimide-mediated crosslinking reactions at −16 °C. The crosslinking density and the mechanical properties of the cryogel matrix could be tuned by adjusting the content of GlcN used during cryogel preparation. In general, incorporation of GlcN did not influence scaffold pore size and ultimate compressive strain but increased porosity. The GH-GlcN16 cryogel showed the highest swelling ratio and degradation rate in hyaluronidase and collagenase solutions. On the contrary, the Young’s modulus, storage modulus, ultimate compressive stress, energy dissipation level, and rate of stress relaxation decreased by increasing the GlcN content in the cryogel. The release of GlcN from the scaffolds in the culture medium of chondrocytes could be sustained for 21 days for GH-GlcN16 in contrast to only 7 days for GH-GlcN9. In vitro cell culture experiments using rabbit articular chondrocytes revealed that GlcN incorporation affected cell proliferation, morphology, and maintenance of chondrogenic phenotype. Overall, GH-GlcN16 showed the best performance in maintaining chondrogenic phenotype with reduced cell proliferation rate but enhanced glycosaminoglycans (GAGs) and type II collagen (COL II) secretion. Quantitative real-time polymerase chain reaction also showed time-dependent up-regulation of cartilage-specific marker genes (COL II, aggrecan and Sox9) for GH-GlcN16. Implantation of chondrocytes/GH-GlcN16 constructs into full-thickness articular cartilage defects of rabbits could regenerate neocartilage with positive staining for GAGs and COL II. The GH-GlcN16 cryogel will be suitable as a scaffold for the treatment of articular cartilage defects.

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“…Besides, the hybrid hydrogel prepared by type I and type II collagen could regulate the performance of the hybrid hydrogels by adjusting the content of two types of collagen. The results showed that the higher the compression modulus of hybrid hydrogel was, the more extracellular matrix the chondrocytes secreted (Yuan et al, 2016).…”

Section: Collagenmentioning

confidence: 99%