Advancements and Frontiers in the High Performance of Natural Hydrogels for Cartilage Tissue Engineering

Bao, Wuren; Li, Menglu; Yang, Yanyu; Wan, Yi; Wang, Xing; Bi, Na; Li, Chunlin

doi:10.3389/fchem.2020.00053

Cited by 94 publications

(83 citation statements)

References 144 publications

(138 reference statements)

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“…It is possible to combine them with synthetic materials in the attempt to reduce the weak mechanical properties, the poor stability and the rapid degradation which represent their mainly limitations. Taken all together these properties indicate the hydrogels as biomaterials ideal for applications in drug delivery technologies, cell encapsulation, contact lenses, scaffolds for tissue engineering, regenerative medicine applications [ 3 , 16 , 21 ], and in the context of angiogenesis, they are also used as 3D cell culture models to better understand the complex mechanisms underlying this process [ 22 ].…”

Section: Natural Hydrogelsmentioning

confidence: 99%

“…Because they all have distinctive characteristics to better suit the final application to achieve different purposes it is possible to choose between various types of hydrogels. Natural hydrogels are usually classified in three main categories: (i) polysaccharides-based, (ii) protein-based and (iii) derived from cellularized tissues [ 16 , 21 , 26 , 27 ].…”

Section: Angiogenic Potential In Natural Hydrogelsmentioning

confidence: 99%

“…Composed by repeated disaccharide units consisting of N-acetylglucosamine and D-glucuronic acid, HA is a linear high molecular weight (about 10 7 kDa) non-sulfated GAG present in the ECM. Its role consists of a space filler, as a lubricant and helps the processes of wound healing, angiogenesis, and signal transduction [ 21 , 27 ]. HA is characterized by a high biodegradability, biocompatibility, high viscoelasticity and hydrophilia, moreover, it can bind water forming hydrogen bonds with the solvent [ 31 ].…”

Section: Angiogenic Potential In Natural Hydrogelsmentioning

confidence: 99%

“…Physical crosslinking ability, good biocompatibility, non-toxicity, and high viscoelasticity are the main properties of alginate hydrogels. Unfortunately, poor stability, poor cell adhesion, and low mechanical strength represent the major limitations [ 4 , 21 ].…”

Section: Angiogenic Potential In Natural Hydrogelsmentioning

confidence: 99%

“…These hydrogels present several advantages such as a good biocompatibility and biodegradability, antibacterial properties, an easy way of controlling degradation, and the possibility of undergoing a sterilization process. On the other hand, inadequate mechanical properties characterize chitosan hydrogels, although it is possible to fix this problem by adding chemical groups or by gelatin crosslinking the hydrogels [ 21 , 26 ].…”

Section: Angiogenic Potential In Natural Hydrogelsmentioning

confidence: 99%

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Angiogenic Potential in Biological Hydrogels

Giraudo

Francesco

Catoira³

et al. 2020

Biomedicines

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Hydrogels are three-dimensional (3D) materials able to absorb and retain water in large amounts while maintaining their structural stability. Due to their considerable biocompatibility and similarity with the body’s tissues, hydrogels are one of the most promising groups of biomaterials. The main application of these hydrogels is in regenerative medicine, in which they allow the formation of an environment suitable for cell differentiation and growth. Deriving from these hydrogels, it is, therefore, possible to obtain bioactive materials that can regenerate tissues. Because vessels guarantee the right amount of oxygen and nutrients but also assure the elimination of waste products, angiogenesis is one of the processes at the base of the regeneration of a tissue. On the other hand, it is a very complex mechanism and the parameters to consider are several. Indeed, the factors and the cells involved in this process are numerous and, for this reason, it has been a challenge to recreate a biomaterial able to adequately sustain the angiogenic process. However, in this review the focal point is the application of natural hydrogels in angiogenesis enhancing and their potential to guide this process.

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Section: Natural Hydrogelsmentioning

confidence: 99%

Section: Angiogenic Potential In Natural Hydrogelsmentioning

confidence: 99%

Section: Angiogenic Potential In Natural Hydrogelsmentioning

confidence: 99%

Section: Angiogenic Potential In Natural Hydrogelsmentioning

confidence: 99%

Section: Angiogenic Potential In Natural Hydrogelsmentioning

confidence: 99%

See 3 more Smart Citations

Angiogenic Potential in Biological Hydrogels

Giraudo

Francesco

Catoira³

et al. 2020

Biomedicines

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Integration of Extracellular Matrices into Organ‐on‐Chip Systems

Kutluk

Bastounis

Constantinou

2023

Adv Healthcare Materials

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The extracellular matrix (ECM) is a complex, dynamic network present within all tissues and organs that not only acts as a mechanical support and anchorage point but can also direct fundamental cell behavior, function, and characteristics. Although the importance of the ECM is well established, the integration of well‐controlled ECMs into Organ‐on‐Chip (OoC) platforms remains challenging and the methods to modulate and assess ECM properties on OoCs remain underdeveloped. In this review, current state‐of‐the‐art design and assessment of in vitro ECM environments is discussed with a focus on their integration into OoCs. Among other things, synthetic and natural hydrogels, as well as polydimethylsiloxane (PDMS) used as substrates, coatings, or cell culture membranes are reviewed in terms of their ability to mimic the native ECM and their accessibility for characterization. The intricate interplay among materials, OoC architecture, and ECM characterization is critically discussed as it significantly complicates the design of ECM‐related studies, comparability between works, and reproducibility that can be achieved across research laboratories. Improving the biomimetic nature of OoCs by integrating properly considered ECMs would contribute to their further adoption as replacements for animal models, and precisely tailored ECM properties would promote the use of OoCs in mechanobiology.

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A Bioglass‐Poly(lactic‐co‐glycolic Acid) Scaffold@Fibrin Hydrogel Construct to Support Endochondral Bone Formation

Jeyachandran

Murshed

Haglund

et al. 2023

Adv Healthcare Materials

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Bone tissue engineering using stem cells to build bone directly on a scaffold matrix often fails due to lack of oxygen at the injury site. This may be avoided by following the endochondral ossification route; herein, a cartilage template is promoted first, which can survive hypoxic environments, followed by its hypertrophy and ossification. However, hypertrophy is so far only achieved using biological factors. This work introduces a Bioglass‐Poly(lactic‐co‐glycolic acid@fibrin (Bg‐PLGA@fibrin) construct where a fibrin hydrogel infiltrates and encapsulates a porous Bg‐PLGA. The hypothesis is that mesenchymal stem cells (MSCs) loaded in the fibrin gel and induced into chondrogenesis degrade the gel and become hypertrophic upon reaching the stiffer, bioactive Bg‐PLGA core, without external induction factors. Results show that Bg‐PLGA@fibrin induces hypertrophy, as well as matrix mineralization and osteogenesis; it also promotes a change in morphology of the MSCs at the gel/scaffold interface, possibly a sign of osteoblast‐like differentiation of hypertrophic chondrocytes. Thus, the Bg‐PLGA@fibrin construct can sequentially support the different phases of endochondral ossification purely based on material cues. This may facilitate clinical translation by decreasing in‐vitro cell culture time pre‐implantation and the complexity associated with the use of external induction factors.

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Advancements and Frontiers in the High Performance of Natural Hydrogels for Cartilage Tissue Engineering

Cited by 94 publications

References 144 publications

Angiogenic Potential in Biological Hydrogels

Angiogenic Potential in Biological Hydrogels

Integration of Extracellular Matrices into Organ‐on‐Chip Systems

A Bioglass‐Poly(lactic‐co‐glycolic Acid) Scaffold@Fibrin Hydrogel Construct to Support Endochondral Bone Formation

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