Effects of commercial selenium products on glutathione peroxidase activity and semen quality in stud boars

Abstract

a b s t r a c tThe aim of this study was to determine how dietary supplementation of inorganic and organic selenium affects the selenium concentration and glutathione peroxidase activity in blood and sperm of sexually mature stud boars. Twenty-four boars of the Large White, Landrace, Pietrain and Duroc breeds of optimal breeding age (on average 2.5 years old) were used. The study lasted 90 days. The boars were randomly assigned to one of three dietary treatment groups: T1 = control; no added selenium (n = 8 boars), T2 = added 0.3 ppm inorganic selenium (sodium selenite, Microgran ® Se 1% BMP) (n = 8 boars), and T3 = added 0.3 ppm organic selenium (Se-yeast, Sel-Plex 2000 ® ) (n = 8 boars). The concentration of selenium was determined in whole blood and semen, while the activity of glutathione peroxidase (GPx) was measured in blood plasma and semen. In order to measure GPx activity in semen, reactivation of the enzymatic GPx activity was performed. The determined selenium concentration in blood was lowest in the non-supplemented group of boars. Blood plasma GPx activity was higher in boars fed organic selenium than in boars fed a diet without supplemented selenium. While the supplementation of sodium selenite significantly increased GPx activity in boar semen. The highest-concentration of selenium in semen at the end of the trial was determined in the group of boars supplemented with organic selenium, somewhat lower in boars fed supplemented inorganic selenium, and the lowest in the non-supplemented group of boars. The only significant difference between the selenite and Se-yeast diet supplementation was observed in the Se concentration of the semen. The supplementation of selenium affected semen quality, and organic selenium improved the progressive motility of the spermatozoa and increased their resistance in hypo-osmotic and thermal tests. The storage ability of short term preserved semen was improved by organic selenium supplementation, as well as also increasing the fertility rate in gilts.

Selenium supplementation during in vitro maturation enhances meiosis and developmental capacity of yak oocytes

“…Our findings were consistent with Petrujkic et al (2014), who determined high GSH-Px activity in blood plasma of boars after Se supplementation. Our findings were consistent with Petrujkic et al (2014), who determined high GSH-Px activity in blood plasma of boars after Se supplementation.…”

“…Moreover, minute fluctuations of trace mineral levels significantly affect biological functions (Navarro-Alarcon & Cabrera-Vique 2008). Selenium (Se), an essential trace element, is necessary for reproduction, immunity, antioxidant systems, embryonic growth and other physiological functions (Ahsan et al 2014;Petrujkic et al 2014). Selenium (Se), an essential trace element, is necessary for reproduction, immunity, antioxidant systems, embryonic growth and other physiological functions (Ahsan et al 2014;Petrujkic et al 2014).…”

Effect of organic and inorganic selenium supplementation on semen quality and blood enzymes in buffalo bulls

“…This might be due to the reason that organic Se as a good source of Se, could be utilized by animals in a more effective way, transported and retained in a good order in target tissues or organs compared to the inorganic Se. Similar findings were reported in rams (Berry et al ), buffalo (El‐Siefy ) and boars (Faye et al ; Petrujkic et al ). Moreover, in goats Kumar et al () reported that Zn and Se levels in blood serum and seminal plasma increased significantly ( P > 0.05) at 60, 75, 90 and 105 days as compared to day 0 in the tested group.…”

Effect of Dietary Selenomethionine Supplementation on Growth Performance, Tissue Se Concentration, and Blood Glutathione Peroxidase Activity in Kid Boer Goats

“…In lambs, organic Se sources (such as selenized yeast and Se-enriched probiotics) are more effective than inorganic Se sources (sodium selenite) in increasing tissue and blood Se Yu-xuan Song and Jin-xing Hou contributed equally to this work. 1 concentrations as well as increasing GSH-Px activitiy in the blood [21]. The dose range, within which the dietary Se supplementation of mature ewes increased blood Se concentrations, was more limited for inorganic Na-selenite than for organic Se-yeast [22].…”

“…Previous studies have shown that Se has an important biochemical function in erythrocyte glutathione peroxidase activity (GSH-Px) [1,2]. Se promotes proper gait [3], healthy pregnancies [4,5], and growth [6,7] in animals.…”