Effects of Coenzyme Q₁₀ on TNF‐α secretion in human and murine monocytic cell lines

Abstract

Studies in humans and cell culture as well as bioinformatics suggested that Coenzyme Q(10) (CoQ10) functions as an anti-inflammatory molecule. Here we studied the influence of CoQ10 (Kaneka Q10) on secretion of the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) by using the human and murine monocytic cell lines THP-1 and RAW264.7 expressing human apolipoprotein E3 (apoE3) or pro-inflammatory apoE4. Incubation of cells with physiological (0.1-10 microM) and supra-physiological (> 10 to < 100 microM) concentrations of CoQ10 led to an intracellular accumulation of its reduced form without any cytotoxic effects. Stimulation of cell models with lipopolysaccharide (LPS) resulted in a substantially release of TNF-alpha. When THP-1 cells were pre-incubated with 10 microM CoQ10, the LPS-induced TNF-alpha release was significantly decreased to 72 +/- 32%. This effect is similar to those obtained by 10 microM N-Acetyl-Cysteine, a well known reference antioxidant. In RAW264.7-apoE3 and -apoE4 cells, significant reductions of LPS-induced TNF-alpha secretion to 73.3 +/- 2.8% and 74.7 +/- 8.9% were found with 2.5 microM and 75 microM CoQ10, respectively. In conclusion, CoQ10 has moderate anti-inflammatory effects in two monocytic cell lines which could be mediated by its antioxidant activity.

Nanomicellar formulation of coenzyme Q10 (Ubisol-Q10) effectively blocks ongoing neurodegeneration in the mouse 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model: potential use as an adjuvant treatment in Parkinson's disease

“…A human clinical study investigating the relation between CoQ 10 and inflammation in patients with coronary artery disease reports that oral supplementation of CoQ 10 at 150 mg/day results in decreased expression of inflammatory markers, that is, C-reactive protein, IL-6, and homocysteine (Lee et al, 2012b). Furthermore, data collected from human and rodent cellular models show that CoQ 10 affects gene expression and inhibits the release of IL-6 and TNF-α, consistent with its anti-inflammatory effects (Schmelzer et al, 2007(Schmelzer et al, , 2008. Far less convincing benefits are reported for neurodegenerative diseases, such as PD (Shults and Haas, 2005;Shults et al, 2002;Villalba et al, 2010).…”

Ubiquinol reduces gamma glutamyltransferase as a marker of oxidative stress in humans

“…CoQ 10 is a known modulator of gene expression [4,5] and inflammatory processes [7-9]. Because the reduced form of CoQ 10 , ubiquinol, is a potent antioxidant that protects lipids, DNA and proteins from oxidative damage [12-14], we examined the effect of Q 10 H 2 supplementation on both serum GGT and AST activity as potential markers of oxidative stress.…”

“…Furthermore, CoQ 10 is necessary for pyrimidine biosynthesis while also being a cofactor for uncoupling proteins [3]. It has also been identified as a modulator of gene expression [4-6], inflammatory processes [7-9] and apoptosis [10,11]. The reduced form of CoQ 10 , ubiquinol (Q 10 H 2 ), serves as a potent antioxidant in mitochondria and lipid membranes as well as a regenerator of other lipid soluble antioxidants [12].…”

Coenzyme Q10 supplementation lowers hepatic oxidative stress and inflammation associated with diet-induced obesity in mice

“…CoQ10 is also involved in oxidative phosphorylation as an electron transporter and is therefore involved in energy homeostasis [17][18][19][20]. Data obtained in vitro even suggest its antiinflammatory potency [21][22][23][24][25]. However, the effect of CoQ10 given orally remains only poorly explored in the context of obesity and diabetes.…”